We narrowed to 22,705 results for: his

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pEThT-SAHS1

Plasmid

#90025

Purpose

To express His-tagged SAHS1 in bacteria

Depositor

Takekazu Kunieda

Article

Yamaguchi et al PLoS One. 2012;7(8):e44209.

Insert

SAHS1

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 11, 2017

Availability

Academic Institutions and Nonprofits only

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ARH1-c003

Plasmid

#173093

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

ADPRH (ADPRH Human)

Tags

mCerulean

Expression

Bacterial

Mutation

E2-L357, codon optimized

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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MacroH2A.2-c002

Plasmid

#173089

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

MACROH2A2 (MACROH2A2 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

K167-K372

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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ALC1-c005

Plasmid

#173092

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

CHD1L (CHD1L Human)

Tags

mCerulean

Expression

Bacterial

Mutation

L613-K879, codon optimized

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pJCC_076 AsCas12a R1226A

Plasmid

#171669

Purpose

For bacterial expression of AsCas12a R1226A (RuvC-inhibiting mutation) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

AsCas12a R1226A

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

changed arginine 1226 to alanine

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pLN382 (FuGW-S(HIF1A)p-mKate2 )

Plasmid

#105180

Purpose

synthetic promoter HIF1A drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(HIF1A)p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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AlkB-D135T

Plasmid

#160433

Purpose

A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Thr

Depositor

Tao Pan

Article

Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30

Insert

E. coli AlkB

Tags

His-tag

Expression

Bacterial

Mutation

Changed Aspartic acid 135 to Threonine; deleted t…

Promoter

T7

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pEThT-CAHS1

Plasmid

#90027

Purpose

To express His-tagged CAHS1 in bacteria

Depositor

Takekazu Kunieda

Article

Yamaguchi et al PLoS One. 2012;7(8):e44209.

Insert

CAHS1

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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ARH3-c002

Plasmid

#173095

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

ADPRS (ADPRS Human)

Tags

mCerulean

Expression

Bacterial

Mutation

A14-S363

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pET28-MRPP1

Plasmid

#67862

Purpose

bacterial expression of TRMT10C, full coding sequence (mitoch. form) + N-term. His tag (thrombin site)

Depositor

Walter Rossmanith

Article

Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10

Insert

MRPP1 (TRMT10C Human)

Tags

His

Expression

Bacterial

Mutation

mitoch. form (contains aa 40-403)

Promoter

T7

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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ALC1-c007

Plasmid

#173112

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

CHD1L (CHD1L Human)

Tags

Nanoluc

Expression

Bacterial

Mutation

L613-K879, codon optimized

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLN592 (FuGW-S(USF1)p-mKate2)

Plasmid

#105204

Purpose

synthetic promoter USF1 drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(USF1)p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLN379 (FuGW-S(E2F1)p-mKate2)

Plasmid

#105177

Purpose

synthetic promoter E2F1 drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(E2F1)p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pEThT-SAHS2

Plasmid

#90026

Purpose

To express His-tagged SAHS2 in bacteria

Depositor

Takekazu Kunieda

Article

Yamaguchi et al PLoS One. 2012;7(8):e44209.

Insert

SAHS2

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pLN599 (pFuGW-hUbCp-hSTE1)

Plasmid

#105206

Purpose

ubiquitin C promoter drives STE expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

hUbCp-hSTE1

Use

Lentiviral and Synthetic Biology

Tags

HA tag

Expression

Mammalian

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDDGFP2_leu2d_NRT1.1

Plasmid

#58332

Purpose

Expresses Arabidopsis thaliana NRT1.1 in Saccharomyces cells as a C-terminal GFP his tagged protein

Depositor

Simon Newstead

Article

Parker et al Nature. 2014 Mar 6;507(7490):68-72. doi:

Insert

NRT1.1 (NRT1.1 Mustard Weed)

Tags

GFP-His

Expression

Yeast

Promoter

GAL1

Available Since

Aug. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pLN377 (FuGW-S(SP1)p-mKate2 )

Plasmid

#105176

Purpose

synthetic promoter SP1 drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(SP1)p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pJCC_075 AsCas12a D908A

Plasmid

#171668

Purpose

For bacterial expression of AsCas12a D908A (RuvC-inactivating mutation) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

AsCas12a D908A

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

changed aspartate 908 to alanine

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pETM33_PEX14_Pex14

Plasmid

#178484

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

PEX14_Pex14 (PEX14 Synthetic, Human)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCT

Plasmid

#60620

Purpose

Plasmid encoding iCas9 and tracrRNA on pRS415 backbone

Depositor

Huimin Zhao

Article

Bao et al ACS Synth Biol. 2014 Sep 19.

Inserts

iCas9
tracrRNA

Use

CRISPR

Tags

FLAG and SV40 NLS

Expression

Yeast

Mutation

changed Aspartate 147 to Tyrosine, Proline 411 to…

Promoter

RPR1p and TEF1p

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(-) + SLNCR1

Plasmid

#86816

Purpose

mammalian expression of lncRNA LINC00673/SLNCR1

Depositor

Carl Novina

Article

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

LINC00673 (LINC00673 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

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pCA528 SPASTIN , residues 112-196

Plasmid

#180627

Purpose

Bacterial expression for SPASTIN MIT, residues 112-196. Has N-term HIS-SUMO tag. Used for X-ray crystallography. Internal ID:WISP21-111.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SPASTIN

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 112-196

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLN466 (FuGW-S(cMYC)p-mKate2)

Plasmid

#105189

Purpose

synthetic promoter cMYC drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(cMYC)p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pHIE053 ZF43x6-C mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

Plasmid

#138734

Purpose

Integration Vector, assembled with pHIE045 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x6-Compact

Use

Synthetic Biology

Expression

Mammalian

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pLN351 (FuGW-S(YBX1)p-mKate2 )

Plasmid

#105174

Purpose

synthetic promoter YBX1 drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(YBX1)p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCA528 SPASTIN , residues 108-200

Plasmid

#180628

Purpose

Bacterial expression for SPASTIN MIT, residues 108-200. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-10.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SPASTIN

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 108-200

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLN352 (FuGW-S(Elk1)p-mKate2)

Plasmid

#105175

Purpose

synthetic promoter Elk1 drives mKate2 experssion

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(Elk1)p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-hDcp2 E147Q, E148Q

Plasmid

#72215

Purpose

bacterial expression of His-tagged human Dcp2 Q147/8 mutant (disrupts the decapping function of Dcp2)

Depositor

Megerditch Kiledjian

Article

Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

Insert

Dcp2 (DCP2 Human)

Tags

His

Expression

Bacterial

Mutation

E147Q, E148Q

Promoter

T7

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only
pETSUMO-TtAgocatdead

Plasmid

#210322

Purpose

Bacterial expression of the His- and SUMO-tagged catalytically inactive Argonaute protein of Thermus thermophilus

Depositor

Phillip Zamore

Article

Jolly et al Cell. 2020 Sep 17;182(6):1545-1559.e18.

Insert

TtAgo (TT_P0026 Thermus thermophilus)

Tags

6x His tag and SUMO tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 STAT3

Plasmid

#67120

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

STAT3 (STAT3 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only