We narrowed to 15,062 results for: pET

1. pRK5 FLAG 17xS/T->A, catalytic dead lipin 1

   Plasmid

   #32008

   Depositor

   David Sabatini

   Article

   Peterson et al Cell. 2011 Aug 5;146(3):408-20.

   Insert

   Lipin1 (Lpin1 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D712E, D714E AND S106A, S150A, (S281A, T282A), S2…

   Promoter

   CMV, SP6

   Available Since

   Oct. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

2. Desmoplakin II tension sensor (FL-based)

   Plasmid

   #118721

   Purpose

   The FL-based human desmoplakin II tension sensor detects forces in the range of 3-5 pN by changes in FRET between YPet(short) and mCherry.

   Depositor

   Carsten Grashoff

   Article

   Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

   Insert

   human Desmoplakin II-[YPet(short)-FL-mCherry] (internal-1353) (DSP Human)
   

   Use

   Retroviral

   Tags

   YPet(short)-FL-mCherry

   Expression

   Mammalian

   Mutation

   inserted FL-based tension sensor module after aa1…

   Promoter

   CMV

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLNHA-C1-HsGIGYF2_AE

   Plasmid

   #148550

   Purpose

   Mammalian Expression of HsGIGYF2

   Depositor

   Elisa Izaurralde

   Article

   Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

   Insert

   HsGIGYF2 (GIGYF2 Human)
   

   Expression

   Mammalian

   Mutation

   one silent mutation and one deletion of Q1211 com…

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pT7-EGFP-C1-HsGIGYF2_AE

   Plasmid

   #148577

   Purpose

   Mammalian Expression of HsGIGYF2

   Depositor

   Elisa Izaurralde

   Article

   Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

   Insert

   HsGIGYF2 (GIGYF2 Human)
   

   Expression

   Mammalian

   Mutation

   one silent mutation and one deletion of Q1211 com…

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Desmoplakin II no force control (FL-based)

   Plasmid

   #118724

   Purpose

   The no force control for the FL-based human desmoplakin II tension sensor serves to detect changes in FRET between YPet(short) and mCherry that are tension-independent.

   Depositor

   Carsten Grashoff

   Article

   Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

   Insert

   human Desmoplakin II (1-1353)-[YPet(short)-FL-mCherry] (DSP Human)
   

   Use

   Retroviral

   Tags

   YPet(short)-FL-mCherry

   Expression

   Mammalian

   Mutation

   truncation after aa1353

   Promoter

   CMV

   Available Since

   Dec. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pT7-MS2-HA-C1-HsGIGYF2_1-718_AI

   Plasmid

   #148960

   Purpose

   Mammalian Expression of HsGIGYF2_1-718

   Depositor

   Elisa Izaurralde

   Article

   Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

   Insert

   HsGIGYF2_1-718 (GIGYF2 Human)
   

   Expression

   Mammalian

   Mutation

   one silent mutation and one deletion of Q1211 com…

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pT7-EGFP-C1-HsGIGYF2_1-718_AI

   Plasmid

   #148948

   Purpose

   Mammalian Expression of HsGIGYF2_1-718

   Depositor

   Elisa Izaurralde

   Article

   Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

   Insert

   HsGIGYF2_1-718 (GIGYF2 Human)
   

   Expression

   Mammalian

   Mutation

   one silent mutation and one deletion of Q1211 com…

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pT7-EGFP-C1-HsGIGYF2_1-718-YFWFtoAAAA_AI

   Plasmid

   #148950

   Purpose

   Mammalian Expression of HsGIGYF2_1-718-YFWFtoAAAA

   Depositor

   Elisa Izaurralde

   Article

   Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

   Insert

   HsGIGYF2_1-718-YFWFtoAAAA (GIGYF2 Human)
   

   Expression

   Mammalian

   Mutation

   one silent mutation and one deletion of Q1211 com…

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pT7-EGFP-C1-HsGIGYF2_280-310_AI

   Plasmid

   #148951

   Purpose

   Mammalian Expression of HsGIGYF2_280-310

   Depositor

   Elisa Izaurralde

   Article

   Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

   Insert

   HsGIGYF2_280-310 (GIGYF2 Human)
   

   Expression

   Mammalian

   Mutation

   one silent mutation and one deletion of Q1211 com…

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pT7-EGFP-C1-HsGIGYF2-F300AF306A_AI

    Plasmid

    #148952

    Purpose

    Mammalian Expression of HsGIGYF2-F300AF306A

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

    Insert

    HsGIGYF2-F300AF306A (GIGYF2 Human)
    

    Expression

    Mammalian

    Mutation

    one silent mutation and one deletion of Q1211 com…

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pT7-EGFP-C1-HsGIGYF2-W288A_AI

    Plasmid

    #148953

    Purpose

    Mammalian Expression of HsGIGYF2-W288A

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

    Insert

    HsGIGYF2-W288A (GIGYF2 Human)
    

    Expression

    Mammalian

    Mutation

    one silent mutation and one deletion of Q1211 com…

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pT7-EGFP-C1-HsGIGYF2-W288AF300AF306A_AI

    Plasmid

    #148954

    Purpose

    Mammalian Expression of HsGIGYF2-W288AF300AF306A

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

    Insert

    HsGIGYF2-W288AF300AF306A (GIGYF2 Human)
    

    Expression

    Mammalian

    Mutation

    one silent mutation and one deletion of Q1211 com…

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pT7-EGFP-C1-HsGIGYF2-YYMLtoAAAA_AF

    Plasmid

    #148691

    Purpose

    Mammalian Expression of HsGIGYF2-YYMLtoAAAA

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

    Insert

    HsGIGYF2-YYMLtoAAAA (GIGYF2 Human)
    

    Expression

    Mammalian

    Mutation

    one silent mutation and one deletion of Q1211 com…

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pXPR_BRD003 FDX1-1

    Plasmid

    #184476

    Purpose

    Lentivirus gRNA targeting human FDX1 gene

    Depositor

    Peter Tsvetkov, Todd Golub

    Article

    Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

    Insert

    FDX1 (FDX1 Human)
    

    Use

    Lentiviral

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pXPR_BRD003 LIPT1-2

    Plasmid

    #184481

    Purpose

    Lentivirus gRNA targeting human LIPT1 gene

    Depositor

    Peter Tsvetkov, Todd Golub

    Article

    Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

    Insert

    LIPT1 (LIPT1 Human)
    

    Use

    Lentiviral

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pXPR_BRD003 LIPT1-1

    Plasmid

    #184480

    Purpose

    Lentivirus gRNA targeting human LIPT1 gene

    Depositor

    Peter Tsvetkov, Todd Golub

    Article

    Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

    Insert

    LIPT1 (LIPT1 Human)
    

    Use

    Lentiviral

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pXPR_BRD003 LIPT2-2

    Plasmid

    #184483

    Purpose

    Lentivirus gRNA targeting human LIPT2 gene

    Depositor

    Peter Tsvetkov, Todd Golub

    Article

    Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

    Insert

    LIPT2 (LIPT2 Human)
    

    Use

    Lentiviral

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pXPR_BRD003 FDX1-2

    Plasmid

    #184477

    Purpose

    Lentivirus gRNA targeting human FDX1 gene

    Depositor

    Peter Tsvetkov, Todd Golub

    Article

    Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

    Insert

    FDX1 (FDX1 Human)
    

    Use

    Lentiviral

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pXPR_BRD003 LIPT2-1

    Plasmid

    #184482

    Purpose

    Lentivirus gRNA targeting human LIPT2 gene

    Depositor

    Peter Tsvetkov, Todd Golub

    Article

    Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

    Insert

    LIPT2 (LIPT2 Human)
    

    Use

    Lentiviral

    Available Since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pnEA-NpM-HsGIGYF2_35-105-E46AE47FN91A-GB1_AF

    Plasmid

    #148686

    Purpose

    Bacterial Expression of HsGIGYF2_35-105-E46AE47FN91A

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

    Insert

    HsGIGYF2_35-105-E46AE47FN91A (GIGYF2 Human)
    

    Expression

    Bacterial

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. AAVS1-iSAM

    Plasmid

    #211495

    Purpose

    AAVS1 targeting vector containing an all-in-one DOX-inducible system for CRISPRa

    Depositor

    Lesley Forrester, Antonella Fidanza

    Article

    Petazzi et al Elife. 2024 Dec 23;13:RP94884. doi: 10.7

    Inserts

    UniSAM insert
    TET-ON
    Neo Resistance
    Insulator
    

    Use

    CRISPR and Synthetic Biology

    Tags

    T2A-mCherry Tag

    Expression

    Mammalian

    Promoter

    EF1a, NA, Splicing acceptor, and TREGV promoter

    Available Since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCDNA3.1 hSCN5AdeltaSIV GFP Nter

    Plasmid

    #145770

    Purpose

    expresses human Nav1.5 lacking the SIV motif and tagged with GFP at its N-terminus in mammalian cells

    Depositor

    Hugues Abriel

    Article

    Petitprez et al Circ Res. 2011 Feb 4;108(3):294-304. doi

    Insert

    sodium voltage-gated channel alpha subunit 5 (SCN5A Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Stop codon at Serine 2013

    Promoter

    CMV

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Desmoplakin II tension sensor (F40-based)

    Plasmid

    #118715

    Purpose

    The F40-based human desmoplakin II tension sensor detects forces in the range of 1-6 pN by changes in FRET between YPet(short) and mCherry.

    Depositor

    Carsten Grashoff

    Article

    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

    Insert

    human Desmoplakin II-[YPet(short)-F40-mCherry] (internal-1353) (DSP Human)
    

    Use

    Retroviral

    Tags

    YPet(short)-F40-mCherry

    Expression

    Mammalian

    Mutation

    inserted F40-based tension sensor module after aa…

    Promoter

    CMV

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDONR221-SARS-CoV-2 S1 RBD

    Plasmid

    #167013

    Purpose

    Gateway-compatible Entry vector encoding spike (S1) RBD domain from SARS-CoV-2

    Depositor

    Spyros Petrakis

    Article

    Gkekas et al J Chem Inf Model. 2024 Nov 13. doi: 10.1

    Insert

    S (S SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Expression

    Mammalian

    Mutation

    No stop codon

    Available Since

    April 5, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. Desmoplakin I tension sensor (F40-based)

    Plasmid

    #118725

    Purpose

    The F40-based human desmoplakin I tension sensor detects forces in the range of 1-6 pN by changes in FRET between YPet(short) and mCherry.

    Depositor

    Carsten Grashoff

    Article

    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

    Insert

    human Desmoplakin I-[YPet(short)-F40-mCherry] (internal-1952) (DSP Human)
    

    Use

    Retroviral

    Tags

    YPet(short)-F40-mCherry

    Expression

    Mammalian

    Mutation

    inserted F40-based tension sensor module after aa…

    Promoter

    CMV

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pXPR_BRD003 LIAS-1

    Plasmid

    #184478

    Purpose

    Lentivirus gRNA targeting human LIAS gene

    Depositor

    Peter Tsvetkov, Todd Golub

    Article

    Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

    Insert

    LIAS (LIAS Human)
    

    Use

    Lentiviral

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pXPR_BRD003 LIAS-2

    Plasmid

    #184479

    Purpose

    Lentivirus gRNA targeting human LIAS gene

    Depositor

    Peter Tsvetkov, Todd Golub

    Article

    Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

    Insert

    LIAS (LIAS Human)
    

    Use

    Lentiviral

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Desmoplakin II no force control (F40-based)

    Plasmid

    #118716

    Purpose

    The no force control for the F40-based human desmoplakin II tension sensor serves to detect changes in FRET between YPet(short) and mCherry that are tension-independent.

    Depositor

    Carsten Grashoff

    Article

    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

    Insert

    human Desmoplakin II (1-1353)-[YPet(short)-F40-mCherry] (DSP Human)
    

    Use

    Retroviral

    Tags

    YPet(short)-F40-mCherry

    Expression

    Mammalian

    Mutation

    truncation after aa1353

    Promoter

    CMV

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pX330-U6-(sgRNA-T2)-Chimeric_BB-CBh-hSpCas9

    Plasmid

    #223323

    Purpose

    Human codon optimized Cas9 expressing plasmid that carries the sgRNA T2 from Mali et al., 2013 (10.1126/science.1232033).

    Depositor

    Jonathan Arias

    Article

    Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do

    Insert

    SpCas9 and sgRNA-T2 (cas9 )
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pT7-EGFP-C1-HsGIGYF2_1-718-del280-310_AI

    Plasmid

    #148949

    Purpose

    Mammalian Expression of HsGIGYF2_1-718-del280-310

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

    Insert

    HsGIGYF2_1-718-del280-310 (GIGYF2 Human)
    

    Expression

    Mammalian

    Mutation

    one silent mutation and one deletion of Q1211 com…

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge