We narrowed to 88,679 results for: Mal;

1. STT3A pEGFP-N2 (1051)

   Plasmid

   #62025

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

   Insert

   STT3A (STT3A Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

2. Circular 200,100 with 8 bp bulge loops (GAPDH)

   Plasmid

   #170121

   Purpose

   AAV vector carrying a guide RNA targeting the human GAPDH mRNA

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

   Insert

   Circular 200,100 guide RNA with 8bp bulge loops (GAPDH Human)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Human U6

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. cacnb4 (rat) in pMT2 vector

   Plasmid

   #107426

   Purpose

   Expresses Calcium channel beta4 auxillary subunit in mammalian cells

   Depositor

   Annette Dolphin

   Article

   Brodbeck et al J Biol Chem. 2002 Mar 8;277(10):7684-93.

   Insert

   cacnb4 (Cacnb4 Rat)
   

   Expression

   Mammalian

   Promoter

   Ad MLP

   Available Since

   March 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST-Flag-FBW7K404R

   Plasmid

   #200717

   Purpose

   The plasmid expresses Flag-tagged FBW7 human isoform 1 with Lys404 to Arg mutation. Used for mammalian overexpression and detection by western blotting.

   Depositor

   Omar Khan

   Article

   Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:

   Insert

   FBW7 (FBXW7 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   FBW7 K404 is mutated to Alanine.

   Promoter

   CMV

   Available Since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS-PHFF-sh-mBVRA

   Plasmid

   #100286

   Purpose

   Expression vector for phycocyanobilin (PCB) synthesis and shRNA for mouse BVRA gene in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

   Inserts

   MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
   shRNA for mouse BVRA
   

   Tags

   FLAG, T7 and Mitochondria-targeting sequence (MTS…

   Expression

   Mammalian

   Mutation

   All genes are codon-optimized for expression in h…

   Promoter

   CAG promoter and H1 promoter

   Available Since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLX304-BCL-XL-Y195F

   Plasmid

   #203573

   Purpose

   Expresses V5-tagged BCL-XL with partial drug resistance to A-1331852 in mammalian cells.

   Depositor

   Abhishek Chakraborty

   Article

   Grubb et al Clin Cancer Res. 2022 Nov 1;28(21):4689-

   Insert

   BCL2L1 (BCL2L1 Human)
   

   Use

   Lentiviral

   Tags

   V5-tagged

   Expression

   Mammalian

   Mutation

   Changed Tyrosine 195 to Phenylalanine for partial…

   Promoter

   CMV

   Available Since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLENTI_Hu_ECT2(WT)

   Plasmid

   #136330

   Purpose

   Lentiviral expression of human ECT2 WT

   Depositor

   Guillermo de Cárcer, Marcos Malumbres

   Article

   de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

   Insert

   ECT2 (ECT2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. mHDAC7-Spliced (1-938) pEF6-V5 (MJS_014) [Edit]

   Plasmid

   #73267

   Purpose

   Expression of mouse HDAC7-S (1-938) in mammalian cells (V5 tag)

   Depositor

   Matthew Sweet

   Article

   Shakespear et al J Biol Chem. 2013 Aug 30;288(35):25362-7

   Insert

   HDAC7 (Hdac7 Mouse)
   

   Tags

   His/V5

   Expression

   Mammalian

   Available Since

   Feb. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. mHDAC7-Spliced (1-938) pEF6-Flag (MJS_015)

   Plasmid

   #73268

   Purpose

   [Edit] Expression of mouse HDAC7-S (1-938) in mammalian cells (Flag tag)

   Depositor

   Matthew Sweet

   Article

   Shakespear et al J Biol Chem. 2013 Aug 30;288(35):25362-7

   Insert

   HDAC7 (Hdac7 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Feb. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. Fgf4enhLV (FpG12)

    Plasmid

    #69444

    Purpose

    positive control plasmid for lentiviral reporter expression in ESCs

    Depositor

    Lisa Dailey

    Article

    Murtha et al Nat Methods. 2014 May;11(5):559-65. doi:

    Inserts

    minimal FGF4 promoter
    EGFP
    Ubiquitin promoter
    Hygromycin resistance
    FGF4 enhancer (Fgf4 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMK297 (DHC1-mAID Hygro)

    Plasmid

    #140542

    Purpose

    DHC1 tagging with mAID

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    DHC1 (DYNC1H1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBactin-Akna-mEGFP

    Plasmid

    #196866

    Purpose

    Expression of the centrosomal protein Akna fused to enhanced (E)-GFP

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Akna-mEGFP (Akna Mouse)
    

    Expression

    Mammalian

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLEX-FKBP-Kras-Blasticidin

    Plasmid

    #120714

    Purpose

    Expresses membrane-targeted recruiter protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    KRAS4B (KRAS Human)
    

    Use

    Lentiviral

    Tags

    FKBP and mCherry

    Expression

    Mammalian

    Mutation

    amino acids 158-188

    Promoter

    CMV

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJHG003

    Plasmid

    #126454

    Purpose

    Expresses Cas9-XTEN-dTdT in mammalian cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    Cas9-XTEN-dTdT (DNTT Streptococcus pyogenes, Human)
    

    Expression

    Mammalian

    Mutation

    Added D343E and D345E to inactivate TdT.

    Promoter

    CMV

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMK296 (DHC1-mAID Neo)

    Plasmid

    #140541

    Purpose

    DHC1 tagging with mAID

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    DHC1 (DYNC1H1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA216-CoV2-RBD-beta variant

    Plasmid

    #194598

    Purpose

    Mammalian expression of RBD domain from SARS-CoV-2 beta (B.1.351)

    Depositor

    Ben Murrell

    Article

    Sheward et al Cell Rep Med. 2021 Nov 16;2(11):100450.

    Insert

    CoV2-RBD-beta variant (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCEP4-myc-S (B.1.351 lineage / South African variant)

    Plasmid

    #169849

    Purpose

    Mammalian expression plasmid for S of SARS-CoV-2 (B.1.351 lineage / South African variant) with N-terminal c-myc tag

    Depositor

    Erik Procko

    Article

    Zhang et al Nat Chem Biol. 2022 Mar;18(3):342-351. d

    Insert

    Protein S
    

    Tags

    HA leader and c-myc tag

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available Since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. mHDAC7-Unspliced (23-938) pEF6-V5 (MJS_017)

    Plasmid

    #73266

    Purpose

    Expression of mouse HDAC7-U (23-938) in mammalian cells (V5 tag)

    Depositor

    Matthew Sweet

    Article

    Shakespear et al J Biol Chem. 2013 Aug 30;288(35):25362-7

    Insert

    HDAC7 (Hdac7 Mouse)
    

    Tags

    His/V5

    Expression

    Mammalian

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP C1-Eps8 ∆cap/∆bund

    Plasmid

    #74891

    Purpose

    mammalian expression of the capping and bundling activity mutant Eps8 fused to GFP

    Depositor

    Giorgio Scita

    Article

    Hertzog et al PLoS Biol. 2010 Jun 1;8(6):e1000387. doi

    Insert

    Eps8 (Eps8 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    V689D, L693D, L757A, K759A

    Available Since

    May 3, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pChuk

    Plasmid

    #87033

    Purpose

    express IKKalpha in mammalian cells

    Depositor

    Georgios Stathopoulos

    Article

    Marazioti et al Nat Commun. 2018 Feb 14;9(1):672. doi: 1

    Insert

    IKKalpha (Chuk Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    July 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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