We narrowed to 24,461 results for: nes

1. AAEL003877-Cas9

   Plasmid

   #100581

   Purpose

   The promoter of AAEL003877 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

   Depositor

   Omar Akbari

   Article

   Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

   Inserts

   AAEL003877-hspcas9-GFP
   Opie-dsRed
   

   Use

   CRISPR

   Tags

   GFP and dsRED

   Expression

   Insect

   Mutation

   Promoter

   AAEL003877 and Opie2

   Available since

   Oct. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. SEP-Nanoluc-cyOFP1

   Plasmid

   #141082

   Purpose

   prototype of the bioluminescent reporter pHLuc, for detecting extracellular pH changes in tumor acidosis

   Depositor

   Jeak Ling Ding

   Article

   Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

   Insert

   SEP-Nanoluc-cyOFP1
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Deletion of amino acids DISGG from positions 673 …

   Promoter

   EF1α

   Available since

   June 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. VGFR1_HUMAN_D0

   Plasmid

   #79743

   Purpose

   This plasmid encodes the kinase domain of VGFR1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   VGFR1
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

4. psgRNA (GB0645)

   Plasmid

   #68210

   Purpose

   Provides a Short guide RNA which is the combination of the bacterial crRNA and tracrRNA into a single guide transcript; it does not contain any target site

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   SgRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEasyG3_nat

   Plasmid

   #184918

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_nat recombines in vivo with a PCR product from pEasyG3_mic.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEasyG3_mic

   Plasmid

   #184920

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_mic recombines in vivo with a PCR product from pEasyG3_zeo/nat/hph.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLX302-MKP7-V5 puro

   Plasmid

   #87771

   Purpose

   Expresses V5 tagged human MAPK phosphatase 7 (MKP7/DUSP16)

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   MKP7 (DUSP16 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. KIF5C(1-560)-DmrC

   Plasmid

   #61673

   Purpose

   Non-fluorescent, truncated kinesin-1 fused to DmrC linker

   Depositor

   Kristen Verhey

   Article

   Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

   Insert

   KIF5C(1-560)-DmrC (Kif5c Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. MSP2372

   Plasmid

   #73196

   Purpose

   Human expression plasmid for SpCas9(R661A/Q695A/Q926A) variant: CMV-T7-humanSpCas9(R661A, Q695A, Q926A)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 (R661A/Q695A/Q926A)-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   R661A, Q695A, and Q926A in SpCas9

   Promoter

   CMV

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCfB2903(XI-2 MarkerFree)

    Plasmid

    #73275

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-2 (Chr XI: 91575..92913)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. PAK7_HUMAN_D0

    Plasmid

    #79706

    Purpose

    This plasmid encodes the kinase domain of PAK7. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    PAK7 (PAK5 Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pSHS325 - Bacterial expression plasmid for SpCas9 REC3 domain

    Plasmid

    #101205

    Purpose

    Bacterial expression plasmid for SpCas9 REC3 domain

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant K506–Q712
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    K506–Q712

    Promoter

    T7

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pXPA-fapO-RgTALsyn

    Plasmid

    #100946

    Purpose

    Expression of Tyrosine Ammonia Lyase from constitutive GAP promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

    Insert

    RgTALsyn
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Silent mutation to remove internal NdeI site on T…

    Promoter

    GAP (Constitutive)

    Available since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCfB3039(XII-2 MarkerFree)

    Plasmid

    #73279

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-2 (Chr XII: 808805..809939)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MK13_HUMAN_D0

    Plasmid

    #79693

    Purpose

    This plasmid encodes the kinase domain of MK13. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    MK13
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

16. tet pLKO.1-shCSE1L v1 puro

    Plasmid

    #192345

    Purpose

    Lentiviral expression vector for an inducible shCSE1L v1

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    shCSE1L v1 (CSE1L Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. tet pLKO.1-shCSE1L v2 puro

    Plasmid

    #192346

    Purpose

    Lentiviral expression vector for an inducible shCSE1L v2)

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    shCSE1L v2 (CSE1L Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. MSCV FLIP p53

    Plasmid

    #19745

    Depositor

    Richard Hynes

    Article

    Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

    Insert

    oligo targeting p53 (Trp53 Mouse)
    

    Use

    Cre/Lox, RNAi, and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. KIF5C(1-560)-mCitrine

    Plasmid

    #61676

    Purpose

    Single mCitrine-labeled, truncated kinesin-1

    Depositor

    Kristen Verhey

    Article

    Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

    Insert

    KHC(1-560)-mCitrine (Kif5c Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. KIF5C(1-560)-GFP11

    Plasmid

    #61670

    Purpose

    Non-fluorescent, truncated kinesin-1 fused to split GFP linker

    Depositor

    Kristen Verhey

    Article

    Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

    Insert

    KIF5C(1-560)-GFP11 (Kif5c Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge