We narrowed to 16,140 results for: igh

1. pBR_attB(bxb)_ccdB_lox_v2

   Plasmid

   #198040

   Purpose

   Bxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Nat Protoc. 2024 Aug 23. doi: 10.1038/s4

   Type

   Empty backbone

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. SpCas9-CBE6c-IVT

   Plasmid

   #215835

   Purpose

   Template for in vitro transcription of CBE6c (with SpCas9)

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6c-SpCas9-2xUGI
   

   Use

   In vitro transcription template

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDest-635

   Plasmid

   #159582

   Purpose

   Gateway Destination vector for protein expression in baculovirus

   Depositor

   Dominic Esposito

   Article

   Yan et al Cell Rep. 2020 Jul 21;32(3):107909. doi:

   Type

   Empty backbone

   Use

   Tags

   His6

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHCMV-10A1

   Plasmid

   #15805

   Depositor

   Miguel Sena-Esteves

   Article

   Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

   Insert

   10A1 Murine leukemia virus envelope mRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 21, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKIR1.0

   Plasmid

   #86409

   Purpose

   CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.

   Depositor

   Tetsuya Higashiyama

   Article

   Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

   Insert

   human-codon-optimized SpCas9
   

   Use

   CRISPR

   Tags

   FLAG and NLS

   Expression

   Mutation

   Promoter

   AtRPS5A

   Available since

   Feb. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. AP2u2-mCherry

   Plasmid

   #27672

   Depositor

   Christien Merrifield

   Article

   Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

   Insert

   Ap2m1 (Ap2m1 Rat)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRBC-sfGFP wild type

   Plasmid

   #174075

   Purpose

   Plasmid with p15a origin of replication expressing codon optimized superfolder GFP with C-terminal His6 tag, under T7 promoter

   Depositor

   Ryan Mehl

   Article

   Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

   Insert

   Superfolder GFP
   

   Use

   Tags

   His-6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR myc-MBP-SspB-iRFP-NLS

   Plasmid

   #223286

   Purpose

   An SspB-tagged maltose binding protein (MBP) with an infrared fluorescent protein for visualization. Used to demonstrate condensate dissolution using the OptoMBP system.

   Depositor

   Jared Toettcher

   Article

   Brumbaugh-Reed et al bioRxiv 2024.01.16.575860

   Insert

   Myc-MBP-SspB-irFP-NLS
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28b-irp3 WT

   Plasmid

   #87106

   Purpose

   expresses Irp3 WT, the thiazolinyl imine reductase from Yersinia enterocolitica

   Depositor

   Audrey Lamb

   Article

   Meneely et al Biochemistry. 2012 Nov 6;51(44):9002-13.

   Insert

   irp3 WT
   

   Use

   Tags

   his-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET21a - NR

    Plasmid

    #89625

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi nitrate reductase in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Nitrate reductase
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-Tat101(AD8)-Flag

    Plasmid

    #115777

    Purpose

    pCMV-Tat101AD8-Flag allows the expression of Tat101(AD8) protein with a C-terminal flag-tag.

    Depositor

    Damian Purcell

    Article

    Khoury et al J Vis Exp. 2019 Jan 22;(143). doi: 10.37

    Insert

    HIV-1 Tat protein (AD8)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR SFFVp Phy-mCherry-CAAX

    Plasmid

    #50839

    Purpose

    Optogenetic PI3K activation system; membrane component

    Depositor

    Wendell Lim

    Article

    Toettcher et al Nat Methods. 2011 Sep 11;8(10):837-9. do

    Insert

    PhyB 1-908
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWRS_1001

    Plasmid

    #192205

    Purpose

    lentiviral expression of SpCas9 and two pol III promoters for dual expression of sgRNAs

    Depositor

    William Sellers

    Article

    Li et al Nat Commun. 2022 May 5;13(1):2469. doi:

    Type

    Empty backbone

    Use

    CRISPR, Lentiviral, and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMK391 (mAID-BSD)

    Plasmid

    #121192

    Purpose

    C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    mAID-BSD
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET N6his GFP1-9

    Plasmid

    #182240

    Purpose

    Tripartite split-GFP. E. coli vector. Expression of GFP1-9 detector fragment.

    Depositor

    Stéphanie Cabantous

    Article

    Pedelacq et al Methods Mol Biol. 2019;2025:423-437. doi

    Insert

    GFP1-9
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHR-mCh-Cry2olig

    Plasmid

    #101222

    Purpose

    Cry2olig(E490G)

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

    Insert

    Cry2olig
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    E490G

    Promoter

    SFFV

    Available since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mApple-pBAD

    Plasmid

    #54536

    Purpose

    Localization: Protein Expression Vector , Excitation: 568, Emission: 592

    Depositor

    Roger Tsien, Michael Davidson, Nathan Shaner

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Type

    Empty backbone

    Use

    Tags

    6xHis and mApple

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pYPQ142-ZmUbi-tRNA

    Plasmid

    #158578

    Purpose

    Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 and pYPQ132-tRNA2.0; assembly of 2 gRNAs

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Maize ubiquitin 1

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Myc-TEAD4

    Plasmid

    #24638

    Depositor

    Kunliang Guan

    Article

    Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

    Insert

    TEAD-4 (TEAD4 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mTagBFP2-SiT-N-15

    Plasmid

    #55325

    Purpose

    Localization: Golgi TGN, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    SiT (ST6GAL1 Human)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    aa 1-45 of SiT

    Promoter

    CMV

    Available since

    Oct. 24, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

21. human syndecan 2 wt

    Plasmid

    #64970

    Purpose

    Constitutive expression in mammalian cells

    Depositor

    Enric Espel

    Article

    Rovira-Clave et al Cell Signal. 2014 Jul;26(7):1489-99. doi

    Insert

    syndecan 2 (SDC2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

22. pcDNA5-CEP41_Halo

    Plasmid

    #135446

    Purpose

    Mamalian cell expression of HaloTag7 localized at the centrosome

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Commun. 2019 Oct 8;10(1):4580. doi:

    Insert

    CEP41-HaloTag7
    

    Use

    Tags

    CEP41

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TetO

    Available since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. eNme2C-CBE6b

    Plasmid

    #215830

    Purpose

    Express CBE6b (with eNme2-C variant) in mammalian cells

    Depositor

    David Liu

    Article

    Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

    Insert

    CBE6b-eNme2C-2xUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET30-R5-GFP

    Plasmid

    #17838

    Depositor

    Jay Keasling

    Article

    Shaikh et al Anal Chem. 2008 Feb 1. 80(3):886-90.

    Insert

    pET30-GFP
    

    Use

    Synthetic Biology

    Tags

    His, R5, and S-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSQT817

    Plasmid

    #53373

    Purpose

    wildtype Cas9 expression plasmid

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMUT2-pFF-41.5-mCherry

    Plasmid

    #192863

    Purpose

    pMUT2 based vector for sensing oxygen in E. coli Nissle 1917

    Depositor

    Morten Sommer

    Article

    Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

    Insert

    Oxygen inducible promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFUM

    Plasmid

    #133130

    Purpose

    A recombinant plasmid expressing fum (fumarase) gene

    Depositor

    Gregg Beckham

    Article

    Guarnieri et al Appl Environ Microbiol. 2017 Aug 17;83(1

    Insert

    fumarase gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PEP-carboxykinase promoter

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET24b-Amg2

    Plasmid

    #199434

    Purpose

    expresses Amg2 in bacterial cells

    Depositor

    Andrew Woolley

    Article

    Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

    Insert

    Amg2
    

    Use

    Tags

    his tag

    Expression

    Bacterial

    Mutation

    L159K mutation

    Promoter

    T7

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mOrange2-pBAD

    Plasmid

    #54531

    Purpose

    Localization: Protein Expression Vector , Excitation: 549, Emission: 565

    Depositor

    Roger Tsien, Michael Davidson, Nathan Shaner

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Type

    Empty backbone

    Use

    Tags

    6xHis and mOrange2

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. eNme2C-CBE6c

    Plasmid

    #215831

    Purpose

    Express CBE6c (with eNme2-C variant) in mammalian cells

    Depositor

    David Liu

    Article

    Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

    Insert

    CBE6c-eNme2C-2xUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge