We narrowed to 38,427 results for: ANT

1. pBK1314-AAV-EFSNC-dSaCas9-KRAB-MECP2

   Plasmid

   #223159

   Purpose

   Expression of KRAB and truncated MECP2 with dSaCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   KRAB-MECP2 (MECP2 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 204-310 (MECP2)

   Promoter

   EF1a

   Available since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1-VEGFR3-V5/HIS

   Plasmid

   #205618

   Purpose

   Expression of human VEGFR3 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   VEGFR3 receptor tyrosine kinase (FLT4 Human)
   

   Use

   Tags

   V5-His

   Expression

   Mammalian

   Mutation

   T28M, S455R- Please see depositor comment

   Promoter

   CMV

   Available since

   Sept. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUB6_EZH2

   Plasmid

   #84014

   Purpose

   EZH2 minigene to test splicing event

   Depositor

   Robert Bradley

   Article

   Kim et al Cancer Cell. 2015 May 11;27(5):617-30. d

   Insert

   EZH2 (EZH2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UB6

   Available since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. kiCAP-AAV-MyoAAV2E

   Plasmid

   #224441

   Purpose

   Rep/Cap plasmid for the production of MyoAAV 2E, a muscle-tropic AAV capsid in mice.

   Depositor

   Pardis Sabeti

   Article

   Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

   Insert

   AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
   

   Use

   AAV

   Tags

   Expression

   Mutation

   RGDQGYQ insert between amino acids 588 and 589 of…

   Promoter

   p41

   Available since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. kiCAP-AAV-MyoAAV3D

   Plasmid

   #224445

   Purpose

   Rep/Cap plasmid for the production of MyoAAV 3D, a muscle-tropic AAV capsid in mice and macaques.

   Depositor

   Pardis Sabeti

   Article

   Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

   Insert

   AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
   

   Use

   AAV

   Tags

   Expression

   Mutation

   RGDYREL insert between amino acids 588 and 589 of…

   Promoter

   p41

   Available since

   Sept. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. kiCAP-AAV-MyoAAV3E

   Plasmid

   #224446

   Purpose

   Rep/Cap plasmid for the production of MyoAAV 3E, a muscle-tropic AAV capsid in mice and macaques.

   Depositor

   Pardis Sabeti

   Article

   Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

   Insert

   AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
   

   Use

   AAV

   Tags

   Expression

   Mutation

   RGDHGVL insert between amino acids 588 and 589 of…

   Promoter

   p41

   Available since

   Sept. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. kiCAP-AAV-MyoAAV4B

   Plasmid

   #224449

   Purpose

   Rep/Cap plasmid for the production of MyoAAV 4B, a muscle-tropic AAV capsid in mice and macaques.

   Depositor

   Pardis Sabeti

   Article

   Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

   Insert

   AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
   

   Use

   AAV

   Tags

   Expression

   Mutation

   RGDYTSV insert between amino acids 588 and 589 of…

   Promoter

   p41

   Available since

   Sept. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. kiCAP-AAV-MyoAAV4D

   Plasmid

   #224451

   Purpose

   Rep/Cap plasmid for the production of MyoAAV 4D, a muscle-tropic AAV capsid in mice and macaques.

   Depositor

   Pardis Sabeti

   Article

   Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

   Insert

   AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
   

   Use

   AAV

   Tags

   Expression

   Mutation

   RGDHGVL insert between amino acids 588 and 589 of…

   Promoter

   p41

   Available since

   Sept. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. kiCAP-AAV-MyoAAV4C

   Plasmid

   #224450

   Purpose

   Rep/Cap plasmid for the production of MyoAAV 4C, a muscle-tropic AAV capsid in mice and macaques.

   Depositor

   Pardis Sabeti

   Article

   Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

   Insert

   AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
   

   Use

   AAV

   Tags

   Expression

   Mutation

   RGDYTSM insert between amino acids 588 and 589 of…

   Promoter

   p41

   Available since

   Sept. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. kiCAP-AAV-MyoAAV3C

    Plasmid

    #224444

    Purpose

    Rep/Cap plasmid for the production of MyoAAV 3C, a muscle-tropic AAV capsid in mice and macaques.

    Depositor

    Pardis Sabeti

    Article

    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

    Insert

    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    

    Use

    AAV

    Tags

    Expression

    Mutation

    RGDYSSV insert between amino acids 588 and 589 of…

    Promoter

    p41

    Available since

    Sept. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. kiCAP-AAV-MyoAAV3B

    Plasmid

    #224443

    Purpose

    Rep/Cap plasmid for the production of MyoAAV 3B, a muscle-tropic AAV capsid in mice and macaques.

    Depositor

    Pardis Sabeti

    Article

    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

    Insert

    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    

    Use

    AAV

    Tags

    Expression

    Mutation

    RGDYSGL insert between amino acids 588 and 589 of…

    Promoter

    p41

    Available since

    Sept. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBK1304-AAV-EFSNC-dCjCas9-KRAB-MECP2

    Plasmid

    #223149

    Purpose

    Expression of KRAB and truncated MECP2 with dCjCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    KRAB-MECP2 (MECP2 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 204-310 (MECP2)

    Promoter

    EF1a

    Available since

    Aug. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV.CAG-FLEX.SF-Venus-iGluSnFR.A184S

    Plasmid

    #106189

    Purpose

    Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-Venus-iGluSnFR.A184S
    

    Use

    AAV

    Tags

    Expression

    Mutation

    SFGFP: T203Y, F46L, T65G, S72A GltI: A184S

    Promoter

    CAG

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pYPQ284-RVRR

    Plasmid

    #160327

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with N563R, K569V, N573R and K625R mutations, without promoter

    Depositor

    Yiping Qi

    Article

    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

    Insert

    Mb2Cas12a RVRR variant
    

    Use

    CRISPR; Gateway compatible cas12a entry clone

    Tags

    NLS

    Expression

    Plant

    Mutation

    N563R, K569V, N573R and K625R

    Promoter

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST-N2H-N1

    Plasmid

    #125547

    Purpose

    Gateway compatible N2H assay destination vector with N-terminus tagging of nanoluc fragment1

    Depositor

    Marc Vidal

    Article

    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:

    Type

    Empty backbone

    Use

    In vitro expression

    Tags

    NanoLuc fragment1 attached to n-terminus of Gatew…

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    synthetic promoter containing yeast, mammalian, a…

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDEST-N2H-C1

    Plasmid

    #125549

    Purpose

    Gateway compatible N2H assay destination vector with C-terminus tagging of nanoluc fragment1

    Depositor

    Marc Vidal

    Article

    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:

    Type

    Empty backbone

    Use

    In vitro expression

    Tags

    NanoLuc fragment1 attached to c-terminus of Gatew…

    Expression

    Mammalian and Yeast

    Mutation

    Promoter

    synthetic promoter containing yeast, mammalian, a…

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDEST-N2H-C2

    Plasmid

    #125559

    Purpose

    Gateway compatible N2H assay destination vector with C-terminus tagging of nanoluc fragment2

    Depositor

    Marc Vidal

    Article

    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:

    Type

    Empty backbone

    Use

    In vitro expression

    Tags

    NanoLuc fragment2 attached to c-terminus of Gatew…

    Expression

    Mammalian and Yeast

    Mutation

    Promoter

    synthetic promoter containing yeast, mammalian, a…

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDEST-N2H-N2

    Plasmid

    #125548

    Purpose

    Gateway compatible N2H assay destination vector with N-terminus tagging of nanoluc fragment2

    Depositor

    Marc Vidal

    Article

    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:

    Type

    Empty backbone

    Use

    In vitro expression

    Tags

    NanoLuc fragment2 attached to n-terminus of Gatew…

    Expression

    Mammalian and Yeast

    Mutation

    Promoter

    synthetic promoter containing yeast, mammalian, a…

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-UbC-eGFP

    Plasmid

    #62518

    Purpose

    Expresses GFP under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV. Optimal for tracing axons in tissue clearing procedures.

    Depositor

    Pantelis Tsoulfas, Jae Lee

    Article

    Soderblom et al Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    March 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pnCS_SEPT7_SEPT9_i5-TEV-Strep

    Plasmid

    #174502

    Purpose

    bacterial co-expression of human SEPT7 and of human SEPT9_i5

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Inserts

    SEPTIN7 (SEPTIN7 Human)
    SEPTIN9 (SEPTIN9 Human)
    

    Use

    Tags

    TEV-Strep

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. NLS-4LNDDE488Q-pCDNA3.1(+)

    Plasmid

    #172314

    Purpose

    Expresses ADAR2 Deaminase Domain (DD) hyperactive mutant E488Q fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS)

    Depositor

    Joshua Rosenthal

    Article

    Vallecillo-Viejo et al RNA Biol. 2018 Jan 2;15(1):104-114. doi:

    Insert

    Lambda N peptide - human ADAR2 Deaminase Domain E488Q
    

    Use

    Tags

    6xHis, Flag, and Nuclear Localization Signal of S…

    Expression

    Mammalian

    Mutation

    changed the Glutamic acid 488 in the Deaminase Do…

    Promoter

    CMV

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. G protein alpha-s(alpha3beta5/G226A)

    Plasmid

    #55798

    Purpose

    In addition to the mutations in alpha-s(alpha3beta5), this dominant negative G protein alpha-s mutant contains the G226A mutation, which impairs activating conformation changes in Switch II.

    Depositor

    Catherine Berlot

    Article

    Berlot et al J Biol Chem. 2002 Jun 7;277(23):21080-5.

    Insert

    alpha-s (alpha3beta5/G226A) (Gnas Rat)
    

    Use

    Tags

    internal EE epitope (residues 189-194 in alpha-s …

    Expression

    Mammalian

    Mutation

    N271K, K274D, R280K, T284D, I285T, G226A in alpha…

    Promoter

    CMV

    Available since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBK1303-AAV-EFSNC-dCjCas9-MECP2(204-310)

    Plasmid

    #223148

    Purpose

    Expression of truncated MECP2 with dCjCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    MECP2 (MECP2 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 204-310

    Promoter

    EF1a

    Available since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. LV-AR-AAFF

    Plasmid

    #171218

    Purpose

    Lenti-viral expression of human androgen receptor DNA-binding mutant: K580A-V581A, mutations in the P-box of the zinc finger 1 at the KVFF motif.

    Depositor

    Steven Balk

    Article

    Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:

    Insert

    AR-AAFF (AR Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    AR-K580A-V581A

    Promoter

    EF-1a

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. 2XSmBiT-SARS-CoV-2 S1

    Plasmid

    #173430

    Purpose

    protein expression plasmid of 2XSmBiT-SARS-CoV-2 S1-IgG1 Fc

    Depositor

    David Nemazee

    Article

    Huang et al J Immunol. 2021 Jul 1;207(1):344-351. do

    Insert

    2XSmBiT SARS-CoV-2 S1-IgG1 Fc (S SARS-CoV-2)
    

    Use

    Luciferase

    Tags

    human IgG1 Fc

    Expression

    Mammalian

    Mutation

    furin cleavage site RRAR to GSAS

    Promoter

    CMV

    Available since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSBbi-RP-CoV2/stSpike-puro

    Plasmid

    #161794

    Purpose

    Transposon-based SARS-CoV-2 secretable trimeric Spike stable expression vector for protein production

    Depositor

    Jorge Luis Arias-Arias

    Article

    Arias-Arias et al Biotechnol Rep (Amst). 2023 Mar;37:e0078

    Insert

    SARS-CoV-2 secretable trimeric Spike
    

    Use

    Transposon

    Tags

    6xHis and dTomato

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLX304-BCL-XL-Y195F

    Plasmid

    #203573

    Purpose

    Expresses V5-tagged BCL-XL with partial drug resistance to A-1331852 in mammalian cells.

    Depositor

    Abhishek Chakraborty

    Article

    Grubb et al Clin Cancer Res. 2022 Nov 1;28(21):4689-

    Insert

    BCL2L1 (BCL2L1 Human)
    

    Use

    Lentiviral

    Tags

    V5-tagged

    Expression

    Mammalian

    Mutation

    Changed Tyrosine 195 to Phenylalanine for partial…

    Promoter

    CMV

    Available since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pnEA-vH_His-TEV-SEPT2-msfGFP_SEPT6

    Plasmid

    #174498

    Purpose

    bacterial co-expression of human SEPT2 fused to monomeric superfolder GFP and of human SEPT6

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Inserts

    SEPTIN2 (SEPTIN2 Human)
    SEPTIN6 (SEPTIN6 Human)
    

    Use

    Tags

    His6-TEV and msfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.A184S

    Plasmid

    #106183

    Purpose

    Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Has Service

    AAV1

    Insert

    SF-Venus-iGluSnFR.A184S
    

    Use

    AAV

    Tags

    Expression

    Mutation

    SFGFP: T203Y, F46L, T65G, S72A GltI: A184S

    Promoter

    hSynapsin

    Available since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pnCS_SEPT7_SEPT9_i3-TEV-Strep

    Plasmid

    #174501

    Purpose

    bacterial co-expression of human SEPT7 and of human SEPT9_i3

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Inserts

    SEPTIN7 (SEPTIN7 Human)
    SEPTIN9 (SEPTIN9 Human)
    

    Use

    Tags

    TEV-Strep

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAAV.CAG-FLEX.SF-Venus-iGluSnFR.A184V

    Plasmid

    #106190

    Purpose

    Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-Venus-iGluSnFR.A184V
    

    Use

    AAV

    Tags

    Expression

    Mutation

    SFGFP: T203Y, F46L, T65G, S72A GltI: A184V

    Promoter

    CAG

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pENTR/D-Topo genomic A. thaliana IMPORTIN ALPHA 1

    Plasmid

    #175814

    Purpose

    pENTR plasmid with genomic sequence of Arabidopsis thaliana IMPORTIN ALPHA ISOFORM 1 (AT3G06720) without stop codon for C-terminal epitope tags

    Depositor

    Lennart Wirthmueller, Mark Banfield

    Article

    Wirthmueller et al Plant J. 2015 Jan;81(1):40-52. doi: 10.1

    Insert

    IMPORTIN ALPHA ISOFORM 1 (IMPA-1 Mustard Weed)
    

    Use

    Pentr plasmid for gateway cloning

    Tags

    none

    Expression

    Mutation

    Promoter

    none

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pYPQ289-RVR

    Plasmid

    #138127

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized ErCas12a with D513R, K519V and N523R mutations, without promoter

    Depositor

    Yiping Qi

    Article

    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

    Insert

    ErCas12a RVR variant
    

    Use

    CRISPR; Gateway compatible cas12a entry clone

    Tags

    NLS

    Expression

    Plant

    Mutation

    D513R, K519V and N523R

    Promoter

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pYPQ284-RVR

    Plasmid

    #138117

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with N563R, K569V and N573R mutations, without promoter

    Depositor

    Yiping Qi

    Article

    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

    Insert

    Mb2Cas12a RVR variant
    

    Use

    CRISPR; Gateway compatible cas12a entry clone

    Tags

    NLS

    Expression

    Plant

    Mutation

    N563R, K569V and N573R

    Promoter

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pαH-S-GSAS-deltaH69/V70

    Plasmid

    #171744

    Purpose

    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-deltaH69/V70 variant

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Science. 2021 Jun 24. pii: science.abi62

    Insert

    Spike (S-GSAS-D614G-deltaH69/V70 variant) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.S72A

    Plasmid

    #106185

    Purpose

    Weak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Has Service

    AAV1

    Insert

    SF-Venus-iGluSnFR.S72A
    

    Use

    AAV

    Tags

    Expression

    Mutation

    SFGFP: T203Y, F46L, T65G, S72A GltI: S72A

    Promoter

    hSynapsin

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAAV.CAG-FLEX.SF-Venus-iGluSnFR.S72A

    Plasmid

    #106191

    Purpose

    Weak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-Venus-iGluSnFR.S72A
    

    Use

    AAV

    Tags

    Expression

    Mutation

    SFGFP: T203Y, F46L, T65G, S72A GltI: S72A

    Promoter

    CAG

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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38. pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.A184V

    Plasmid

    #106184

    Purpose

    Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-Venus-iGluSnFR.A184V
    

    Use

    AAV

    Tags

    Expression

    Mutation

    SFGFP: T203Y, F46L, T65G, S72A GltI: A184V

    Promoter

    hSynapsin

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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39. pαH-S-GSAS-B.1.351.v5

    Plasmid

    #173790

    Purpose

    Mammalian expression of SARS-CoV-2 Spike protein South African variant version 5

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Science. 2021 Jun 24. pii: science.abi62

    Insert

    Spike (S-GSAS-B.1.351 variant version 5) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    Available since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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40. pαH-S-GSAS-D614G-N501Y

    Plasmid

    #171748

    Purpose

    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-N501Y variant

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Science. 2021 Jun 24. pii: science.abi62

    Insert

    Spike (S-GSAS-D614G-N501Y variant) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge