We narrowed to 9,862 results for: SUB;
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Plasmid#205468PurposeEnhancer containing plasmid used for assessing enhancer::promoter interactionsDepositorInsertchr5:550kb LG4
UseSynthetic Biology; Subcloning of taq-amplified pc…Available SinceJuly 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-SARS-CoV-2-S-ectodomain
Plasmid#235984PurposeInternally tagged SARS-CoV-2 spike ecto-domainDepositorInsertSpike protein (S SARS-CoV-2)
Tags8xHis and Twin-Strep tag and internal Twin-Strep …ExpressionMammalianMutationaa 1-1208, D614G, RRAR684-684GSAS, and DK987-987P…PromoterCMVAvailable SinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT to BioID-nDIC2-3xFlag
Plasmid#232418PurposeExpresses human dynein subunit DIC2 in mammalian cells.DepositorInsertDynein Intermediate Chain 2 (DYNC1I2 Human)
TagsBioID and Flag-3xExpressionMammalianPromoterCMVAvailable SinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT to BioID-cDIC2-3xFlag
Plasmid#232416PurposeExpresses human dynein subunit DIC2 in mammalian cells.DepositorInsertDynein Intermediate Chain 2 (DYNC1I2 Human)
TagsBioID and Flag-3xExpressionMammalianPromoterCMVAvailable SinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
IL2-mCherry in pmCherryN1
Plasmid#187283PurposeExpress IL2-mCherryDepositorInsertinterleukin-2 receptor alpha-subunit (IL2 Human)
TagsmCherryExpressionMammalianPromoterCMVAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-eGFP:HA-mTREK-1(S131C/A286F)
Plasmid#224775PurposeMammalian expression vector for generating mouse TREK-1DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
ExpressionMammalianMutationS131C / A286FPromoterCMVAvailable SinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1 (S131A/C159A/C219A)
Plasmid#224753PurposeIn vitro transcription for Oocyte expression of mTREK-1 (S131A/C159A/C219A)DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionMutationS131A/C159A/C219APromoterT7Available SinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1 (S131C/C159A/C219A)
Plasmid#224752PurposeIn vitro transcription for Oocyte expression of mTREK-1 (S131C/C159A/C219A)DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionMutationS131C/C159A/C219APromoterT7Available SinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1 (C159A/C219A/C365A/C399A)
Plasmid#224754PurposeIn vitro transcription for Oocyte expression of mTREK-1 (C159A/C219A/C365A/C399A)DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionMutationC159A/C219A/C365A/C399APromoterT7Available SinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR-TMEM144
Plasmid#221426PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertTMEM144 (TMEM144 Human)
ExpressionMammalianAvailable SinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPCaspase
Plasmid#214042PurposeBacterial expression plasmid for PCaspase from Haliangium ochraceumDepositorInsertPCaspase
ExpressionBacterialMutationWTPromoterlacUV5 promoterAvailable SinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
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YIp128-CUP1-His6-CUbo(K48R/K63R)-GFP
Plasmid#212795PurposeCopper-inducible expression of the artificial test substrate His6-CUbo(K48R/K63R)-GFP in budding yeastDepositorInsertHis6-CUbo(K48R/K63R)-GFP
UseIntegrative vectorExpressionYeastMutationCUb(K48R/K63R)PromoterCUP1Available SinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-HAPPID1
Plasmid#204626PurposeExpresses the human anti-Phl p 7 IgD/λ antibody 102.1F10 (HAPPID1) in mammalian cells. HAPPID1 binds to the grass pollen allergen Phl p 7 with subnanomolar affinity.DepositorInsertsHAPPID1 heavy chain
HAPPID1 light chain
ExpressionMammalianPromotermouse elongation factor 1 alpha and rat elongatio…Available SinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBridge-tyrosinase.σ3A
Plasmid#197513PurposeExpression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-3 σ3A fusion protein in yeast (yeast three-hybrid assays)DepositorInsertstyrosinase cytosolic tail
AP-3 σ3A
TagsGAL4-DNA binding domain fragment, HA tag, and nuc…ExpressionYeastMutationsilent substitution in codon 2 of tyrosinase tail…PromoterADH1 and MET25Available SinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-ε
Plasmid#197261PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-4 ε fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)DepositorInsertAP-4 ε (AP4E1 Human)
TagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationsilent substitutions in codons 905, 1129 and 1137PromoterADH1Available SinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-QQN
Plasmid#193563PurposeExpresses human Clathrin Light Chain B mutant (residues 20-22 were substituted from EED to QQN) tagged with mEGFP in mammalian cells.DepositorInsertClathrin light chain B (CLTB Human)
TagsmEGFPExpressionMammalianMutationChanged from EED to QQN at residue 20-22PromoterCMVAvailable SinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV9-hSyn-iDianiSnFR_PM
Plasmid#177759PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertpAAV9-hSyn-iDianiSnFR_PM
UseAAVPromoterhSynAvailable SinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV9-hSyn-iDianiSnFR_ER
Plasmid#177758PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertpAAV9-hSyn-iDianiSnFR_ER
UseAAVPromoterhSynAvailable SinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV9-hSyn-iCyt_F_SnFR_PM
Plasmid#177755PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertpAAV9-hSyn-iCyt_F_SnFR_PM
UseAAVPromoterhSynAvailable SinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV9-hSyn-iCytSnFR_PM
Plasmid#177753PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertpAAV9-hSyn-iCytSnFR_PM
UseAAVPromoterhSynAvailable SinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV9-hSyn-iCytSnFR_ER
Plasmid#177752PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertpAAV9-hSyn-iCytSnFR_ER
UseAAVPromoterhSynAvailable SinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV(MinDis)-iDianiSnFR_PM
Plasmid#177751PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertpCMV(MinDis)-iDianiSnFR_PM
ExpressionMammalianPromoterhCMVAvailable SinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV(MinDis)-iDianiSnFR_ER
Plasmid#177750PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertpCMV(MinDis)-iDianiSnFR_ER
ExpressionMammalianPromoterhCMVAvailable SinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV(MinDis)-iCyt_BrEt_SnFR_PM
Plasmid#177747PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertpCMV(MinDis)-iCyt_BrEt_SnFR_PM
ExpressionMammalianPromoterhCMVAvailable SinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
_pCMV(MinDis)-iCyt_F_SnFR_PM
Plasmid#177745PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsert_pCMV(MinDis)-iCyt_F_SnFR_PM
ExpressionMammalianPromoterhCMVAvailable SinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV(MinDis)-iCytSnFR_PM
Plasmid#177743PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertpCMV(MinDis)-iCytSnFR_PM
ExpressionMammalianPromoterhCMVAvailable SinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV9-hSyn iCyt_BrEt_SnFR_ER
Plasmid#177756PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertpAAV9-hSyn iCyt_BrEt_SnFR_ER
UseAAVPromoterhSynAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV9-hSyn-iCyt_F_SnFR_ER
Plasmid#177754PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertpAAV9-hSyn-iCyt_F_SnFR_ER
UseAAVPromoterhSynAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV(MinDis)-iCyt_BrEt_SnFR_ER
Plasmid#177746PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertpCMV(MinDis)-iCyt_BrEt_SnFR_ER
ExpressionMammalianPromoterhCMVAvailable SinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
_pCMV(MinDis)-iCyt_F_SnFR_ER
Plasmid#177744PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsert_pCMV(MinDis)-iCyt_F_SnFR_ER
ExpressionMammalianPromoterhCMVAvailable SinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV(MinDis)-iCytSnFR_ER
Plasmid#177742PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertpCMV(MinDis)-iCytSnFR_ER
ExpressionMammalianPromoterhCMVAvailable SinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Bmpr2 gRNA resistant
Plasmid#163408PurposeExpresses BMPR-2 resistant to the gRNAs(#163388-90) in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
ExpressionMammalianMutationgRNA-targeting sequences are substituted with nuc…Available SinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Bmpr2ΔEC gRNA resistant
Plasmid#163413PurposeExpresses BMPR-2ΔEC resistant to the gRNAs(#163388-90) in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
ExpressionMammalianMutationThe extracellular domain (27-150 aa) is deleted. …Available SinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Bmpr2ΔTail gRNA resistant
Plasmid#163412PurposeExpresses BMPR-2ΔTail resistant to the gRNAs(#163388-90) in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
ExpressionMammalianMutationThe C-terminal tail domain (530-1038 aa) is delet…Available SinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-Tac-STC
Plasmid#162490PurposeExpresses partial length Tac (stem region, TMD and cytosolic tail) with GFP tag in mammalian cellsDepositorInsertpartial length Tac (stem region, TMD and cytosolic tail) (IL2RA Human)
TagsGFPExpressionMammalianMutationTac is in partial length with its stem region, TM…Available SinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Venus-cpVenus-FLARE-EKAR-EV(T/A)
Plasmid#123340PurposeNegative control mutant for Venus-cpVenus-FLARE-EKAR-EV biosensor.DepositorInsertVenus-cpVenus-FLARE-EKAR-EV(T/A)
Tags6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable SinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Venus-cpVenus-FLARE-CKAR(T/A)
Plasmid#123344PurposeNegative control mutant for Venus-cpVenus-FLARE-CKAR biosensor.DepositorInsertVenus-cpVenus-FLARE-CKAR(T/A)
Tags6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable SinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1_cryst
Plasmid#133271PurposeX. laevis expression vector. It will generate the TREK-1 channelDepositorInsertPotassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 )
UseOocyte expressionMutationaa 21-322, K84R, Q85E, T86K, I88L, A89R, Q90A, A9…Available SinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC37A3
Plasmid#132107PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC37A3 (SLC37A3 Human)
ExpressionMammalianAvailable SinceDec. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits