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We narrowed to 16,181 results for: nes

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Showing: 4261 - 4280 of 16181 results

  2. pAaePUb::Cas9-2A-Neo

    Plasmid
    #176680
    Purpose
    Expression of Drosophila codon optimized spCas9 under Ae. aegypti Polyubiquitin promoter (AAEL003888)
    Depositor
    Norbert Perrimon
    Publication
    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:
    Insert
    spCas9; NeoR (aminoglycoside phosphotransferase from Tn5)
    Use
    Crispr
    Expression
    Insect
    Mutation
    Drosophila codon optimized
    Promoter
    Ae. aegypti poly-ubiquitin (AAEL003888)
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    176680-plasmid-map-sequence-id-348671

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  3. BB3cN_pGAP_23*_pPFK300_Cas9

    Plasmid
    #104911
    Purpose
    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC
    Depositor
    Brigitte Gasser
    Publication
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Jan. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    104911-plasmid-map-sequence-id-269032

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  4. pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-SaCas9WT-2xNLS

    Plasmid
    #107320
    Purpose
    Expresses SpCas9 fused to SaCas9 in mammalian cells
    Depositor
    Scot Wolfe
    Publication
    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-SaCas9 fusion
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    SpCas9 is fused to SaCas9
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    107320-plasmid-map-sequence-id-204686

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  5. pX330-Flag-dSpCas9-HF1

    Plasmid
    #92115
    Purpose
    Expression plasmid for human codon-optimized dead/inactive high-fidelity SpCas9-HF1 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dead/inactive SpCas9-HF1 with FLAG tag
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    D10A, N497A, R661A, Q695A, H840A, Q926A
    Promoter
    Cbh
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    92115-plasmid-map-sequence-id-197505

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  8. CBX6

    Plasmid
    #25296
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CBX6_02 (CBX6 Human)
    Tags
    His
    Expression
    Bacterial
    Available since
    Aug. 16, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    3 Articles

  9. eSpCas9-plus

    Plasmid
    #126767
    Purpose
    Expression plasmid for human codon-optimized increased fidelity eSpCas9-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eSpCas9-plus
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    K848A, R1060A; amino acids 1005-1013 replaced wit…
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126767-plasmid-map-sequence-id-410890

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  12. pV1025

    Plasmid
    #111424
    Purpose
    Expression of Candida albicans codon-optimized Cas9 (CaCas9) - integrates at ENO1 (Part of Duet system)
    Depositor
    Gerald Fink
    Publication
    Vyas et al Sci Adv. 2015;1(3):e1500248.
    Insert
    CaCas9
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Oct. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111424-plasmid-map-sequence-id-212243

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  17. pEGFP-MYO10-HMM

    Plasmid
    #87256
    Purpose
    Expression plasmid to perform NanoSPD assays in mammalian cells (generates EGFP-tagged bait).
    Depositor
    Thomas Friedman
    Publication
    Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MYO10 (Myo10 Mouse)
    Expression
    Mammalian
    Mutation
    Myo10 is truncated to contain aa.1-941
    Promoter
    CMV
    Available since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    87256-plasmid-map-sequence-id-176080

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Showing: 4261 - 4280 of 16181 results