We narrowed to 11,766 results for: nsf

1. pLenti HsATP10B - EmGFP

   Plasmid

   #204474

   Purpose

   transfer plasmid for lentiviral vector production expressing Hs ATP10B WT fused with EmGFP

   Depositor

   Veerle Baekelandt

   Article

   Wouters et al Biochim Biophys Acta Mol Cell Res. 2024

   Inserts

   ATP10B (ATP10B Human)
   EmGFP
   

   Use

   Lentiviral

   Tags

   EmGFP

   Promoter

   CMV

   Available Since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX131

   Plasmid

   #167149

   Purpose

   MoClo-compatible Level 0-SP promoterless vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA codon-optimised for expression in Nicotiana benthamiana and Pichia pastoris

   Depositor

   Karen Sarkisyan

   Article

   Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

   Insert

   4'-phosphopantetheinyl transferase, npgA
   

   Use

   Synthetic Biology

   Available Since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. SIN-cPPT-H1-sgHTT1-U6-sgGFP2-7SK-sgCas9-PGK-mCherry-WPRE

   Plasmid

   #87919

   Purpose

   HIV-1 SIN lentiviral transfer vector

   Depositor

   Nicole Déglon

   Article

   Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

   Insert

   sgHTT1, shGFP2, sgCas9, cherry
   

   Use

   Lentiviral

   Promoter

   H1, U6, 7SK, PGK

   Available Since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pESC-LEU-SopB

   Plasmid

   #183670

   Purpose

   Inducible Salmonella Typhimurium SopB for expression in yeast

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pESC-Leu-SopB(C460S)

   Plasmid

   #183671

   Purpose

   Inducible Salmonella Typhimurium SopB (catalytically inactive) for expression in yeast

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Expression

   Yeast

   Mutation

   C460S

   Promoter

   GAL1

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28-MRPP3-His

   Plasmid

   #67866

   Purpose

   bacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. His tag

   Depositor

   Walter Rossmanith

   Article

   Vilardo et al Nucleic Acids Res. 2012 Dec;40(22):11583

   Insert

   MRPP3 (KIAA0391 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Amino acid 46 of recombinant MRPP3 is preceded by…

   Promoter

   T7

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMXs-hu-c-Myc-dN2

   Plasmid

   #50774

   Purpose

   retroviral expression of human c-Myc dN2 fragment

   Depositor

   Shinya Yamanaka

   Article

   Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

   Insert

   c-Myc -dN2 (MYC Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   N-terminal deletion, contains aa 42-439

   Available Since

   Feb. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMXs-hu-c-Myc-dN1

   Plasmid

   #50773

   Purpose

   retroviral expression of human c-Myc dN1 fragment

   Depositor

   Shinya Yamanaka

   Article

   Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

   Insert

   c-Myc -dN1 (MYC Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   N-terminal deletion, contains aa 14-439

   Available Since

   Feb. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(-)-V5.hOr47a

   Plasmid

   #126474

   Purpose

   Human codon-optimized version of D. melanogaster Or47a (hOr47a) without N-terminal tags for improved intracellular trafficking in mammalian cells

   Depositor

   Bill Hansson

   Article

   Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

   Insert

   Or47a (Or47a Fly)
   

   Tags

   V5 tag

   Expression

   Mammalian

   Mutation

   codon optimization for H. sapiens

   Promoter

   CMV

   Available Since

   Aug. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCI-neo-FHM

    Plasmid

    #51871

    Purpose

    Expresses N-terminal FLAG-HA-tagged full length rat MIBP1 (cloned into pCI-neo)

    Depositor

    Kenshi Hayashi, Tomoko Tahira

    Article

    Iwashita et al J Biol Chem. 2012 Mar 23;287(13):9887-90

    Insert

    MIBP1 (Hivep2 Rat)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Available Since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pS6-C-hα2M-H6

    Plasmid

    #128497

    Purpose

    Expresses human C-α2M (from F789 to S1468) in mammalian cells. With C-t H6x tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    A2M (F789-S1468) (A2M Human)
    

    Tags

    His tag (6x)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC19-U6-BsmBI_cassette-CjeCas9-sgRNA (KAC482)

    Plasmid

    #133793

    Purpose

    U6 promoter sgRNA entry vector used for all CjeCas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V2-TGAA linker sgRNA architecture from Kim et al. Nature Communications 2017

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    CjeCas9 sgRNA entry vector
    

    Expression

    Mammalian

    Mutation

    V2-TGAA linker sgRNA architecture from Kim et al.…

    Promoter

    U6

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. His6-SMT3-SopB

    Plasmid

    #183676

    Purpose

    Recombinant Salmonella Typhimurium SopB

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium, Budding Yeast)
    

    Tags

    His6-S. cerevisiae SMT3(2-101)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. gRNAs[bTub+Sxl].1046B

    Plasmid

    #112692

    Purpose

    express two gRNA targeting bTub & Sxl under dU6-3 promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    U6.3-gRNA[bTub] and U6.3-gRNA[Sxl] (Sxl Fly)
    

    Use

    CRISPR

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST47-ARF4-GFP

    Plasmid

    #67392

    Purpose

    Mammalian expression of hArf4 with C-term GFP tag

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARF4 (ARF4 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    V53A, L123P and M134I mutations were unintended P…

    Promoter

    CMV

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pGK-hMIGA2-DsRed

    Plasmid

    #192867

    Purpose

    Express codon-optimized human MIGA2 full length with a C-terminal DsRed tag in mammalian cells

    Depositor

    Karin Reinisch

    Article

    Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

    Insert

    Codon-optimized human Mitoguardin 2 (MIGA2 Human)
    

    Tags

    DsRed

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO.TRC1.shmNsd1.2, puro

    Plasmid

    #114447

    Purpose

    Lentiviral vector for expression of shRNA sequence targeting mouse Nsd1

    Depositor

    Adrian Bracken

    Article

    Streubel et al Mol Cell. 2018 Apr 19;70(2):371-379.e5.

    Insert

    shNsd1.2 (Nsd1 Mouse)
    

    Use

    Lentiviral and RNAi

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO.TRC1.shmNsd1.1, puro

    Plasmid

    #114446

    Purpose

    Lentiviral vector for expression of shRNA sequence targeting mouse Nsd1

    Depositor

    Adrian Bracken

    Article

    Streubel et al Mol Cell. 2018 Apr 19;70(2):371-379.e5.

    Insert

    shNsd1.1 (Nsd1 Mouse)
    

    Use

    Lentiviral and RNAi

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJHG004

    Plasmid

    #126455

    Purpose

    Expresses dTdT in mammalian cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    dTdT (DNTT Human)
    

    Expression

    Mammalian

    Mutation

    Added D343E and D345E to inactivate the enzyme.

    Promoter

    CMV

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRC/RSV-rat Agt

    Plasmid

    #122104

    Purpose

    Expression of angiotensinogen

    Depositor

    John Chan

    Article

    Zhang et al J Am Soc Nephrol. 2002 Feb;13(2):302-12.

    Insert

    angiotensinogen (Agt Rat)
    

    Expression

    Mammalian

    Promoter

    RSV promoter

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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