We narrowed to 5,351 results for: PID

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pHS484_10xHis_MBP_TEV_dSpRY

Plasmid

#244832

Purpose

Bacterial expression of catalytically inactive SpRY Cas9 (dSpRY) for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

dSpRY Cas9

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Mutation

D10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pHS24_10xHis_MBP_TEV_SpRY-Cas9

Plasmid

#244827

Purpose

Bacterial expression of SpRY Cas9 for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpRY Cas9

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Mutation

A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pHS72_10xHis_MBP_TEV_SpG-Cas9_2xNLS

Plasmid

#244834

Purpose

Bacterial expression of SpG Cas9 with 2xSV40 nuclear localization sequence for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpG Cas9

Use

CRISPR

Tags

10xHis-MBP and 2xSV40 NLS

Expression

Bacterial

Mutation

D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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yTREX-ApR

Plasmid

#177307

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Type

Empty backbone

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pYTRW21K_1Ti1

Plasmid

#177291

Purpose

genomic integration of genes (inserted in I-SceI-site) via rrn-interposon with TcR and eYFP

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

eYFP

Use

Synthetic Biology; Yeast expression, rrn genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pYTSK56K_3G7

Plasmid

#177301

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn7 with GmR and mCherry

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

mCherry

Use

Synthetic Biology; Yeast expression, tn7 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pYTSK58K_6G7

Plasmid

#177302

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn7 with GmR and PE-H

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

pe-h

Use

Synthetic Biology; Yeast expression, tn7 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pYTSK65K_8G7

Plasmid

#177303

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn7 with GmR and GUS

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

uidA

Use

Synthetic Biology; Yeast expression, tn7 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pYTRW14K_7G5

Plasmid

#177283

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with GmR and LacZ

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

lacZ

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pYTRW15K_2G5

Plasmid

#177284

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with GmR and mTagBFP2

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

mTagBFP2

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pYTRW17K_6G5

Plasmid

#177285

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with GmR and PE-H

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

pe-h

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pYTRW16K_1G5

Plasmid

#177286

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with GmR and eYFP

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

eYFP

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pYTRW13K_3G5

Plasmid

#177287

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with GmR and mCherry

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

mCherry

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pYTRW18K_3T5

Plasmid

#177288

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with TcR and mCherry

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

mCherry

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pYTRW28K_0Ti1

Plasmid

#177290

Purpose

genomic integration of genes (inserted in I-SceI-site) via rrn-interposon/SacB with TcR

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

PsacB-sacB

Use

Synthetic Biology; Yeast expression, rrn genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-fDIO(SERT-HA)-3xmiR122-WPRE-HGHpA

Plasmid

#220937

Purpose

FLP-dependent serotonin transporter overexpression vector for non-invasive gap junction tracing

Depositor

Chenghua Gu

Article

Krolak et al Cell. 2025 Jul 11:S0092-8674(25)00732-9.

Insert

FLP-dependent Serotonin Transporter (SLC6A4 Human)

Use

AAV

Tags

3x HA Tag

Available Since

July 16, 2025

Availability

Academic Institutions and Nonprofits only

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pPD1178-LPDV promoterless PuroR-P2A-miRFP720

Plasmid

#201537

Purpose

Landing pad destination vector for mMoClo golden gate-based assembly of landing pad integration vectors; contains BxB1 attB site, promoterless puromycin resistance gene and miRFP720 gene

Depositor

Joshua Leonard

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Insert

Promoterless PuroR-P2A-miRFP720

Expression

Mammalian

Promoter

None

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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TMEM16F-I521A-peGFP-N1

Plasmid

#228577

Purpose

Expresses mouse TMEM16F-I521A-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)

Expression

Mammalian

Mutation

I521A plus a 3 amino acid N-terminal truncation (…

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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TMEM16F-I521E-peGFP-N1

Plasmid

#228578

Purpose

Expresses mouse TMEM16F-I521E-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)

Expression

Mammalian

Mutation

I521E plus a 3 amino acid N-terminal truncation (…

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2puro-E-cadherin(Canis)-exon1 6-28 gRNA

Plasmid

#209922

Purpose

A knock-out vector for the dog CDH1

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

A gRNA targeting the dog CDH1 gene.

Use

CRISPR and Lentiviral

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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