We narrowed to 10,047 results for: yeast

1. pMT957

   Plasmid

   #228293

   Purpose

   Constitutive mutant PhlTA expression with strong promoter

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

   Insert

   phlTA mutant
   

   Use

   Synthetic Biology

   Tags

   Three copies of VP16 (VP48)-Nuclear localization …

   Expression

   Yeast

   Mutation

   E41G

   Promoter

   ScPGK1 promoter

   Available since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMT1035

   Plasmid

   #228300

   Purpose

   Constitutive expression of LuxTA mutant with strong promoter

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

   Insert

   luxTA mutant
   

   Use

   Synthetic Biology

   Tags

   Nuclear localization signal-Three copies of VP16 …

   Expression

   Yeast

   Mutation

   S116Y, W201R

   Promoter

   KpGAPDH promoter

   Available since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRR-ERbeta-5Z

   Plasmid

   #23062

   Depositor

   Charles Miller

   Article

   Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

   Insert

   ERbeta (ESR2 Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pCashGem-Xyl2-URA3-LINEAR

   Plasmid

   #174840

   Purpose

   pRS414 backbone containing LINEAR fragment targeting XYL2 in S. stipitis

   Depositor

   Zengyi Shao

   Article

   Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

   Insert

   Codon optimized Cas9:hGem
   

   Use

   CRISPR and Synthetic Biology

   Tags

   hGeminin tag

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   ENO1p

   Available since

   Jan. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFA6a-kanMX6-P41nmt-3HA

   Plasmid

   #39284

   Depositor

   John Pringle, Jurg Bahler

   Article

   Bahler et al Yeast. 1998 Jul;14(10):943-51.

   Insert

   nmt1 promoter (nmt1 Fission Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   3xHA, A. gossypii translation elongation factor 1…

   Expression

   Mutation

   -1163 to +6 of nmt1 locus with a 4bp deletion (TA…

   Promoter

   Available since

   Aug. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pFA6a-kanMX6-P81nmt1-3HA

   Plasmid

   #39285

   Depositor

   John Pringle, Jurg Bahler

   Article

   Bahler et al Yeast. 1998 Jul;14(10):943-51.

   Insert

   nmt1 promoter (nmt1 Fission Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   3xHA, A. gossypii translation elongation factor 1…

   Expression

   Mutation

   -1163 to +6 of nmt1 locus with a 7bp deletion (AT…

   Promoter

   Available since

   Aug. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pUDC175

   Plasmid

   #103019

   Purpose

   S. cerevisae centromic plasmid harboring Fncpf1 under control of TEF1 promoter

   Depositor

   Jean-Marc Daran

   Article

   Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

   Insert

   Fncpf1
   

   Use

   Tags

   3HA and NLS

   Expression

   Yeast

   Mutation

   human codon optimized

   Promoter

   TEF1

   Available since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pESC-URA::CYP71AV1/CPR

   Plasmid

   #20118

   Depositor

   Jay Keasling

   Article

   Ro et al Nature. 2006 Apr 13. 440(7086):940-3.

   Insert

   amorphadiene oxidase and p450 reductase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 2, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pAG32-3HA-3FLAG

   Plasmid

   #154081

   Purpose

   For C-terminal 3xHA-3xFLAG-protein tagging in S. pombe using hphMX4 as resistance marker.

   Depositor

   Cornelia Kilchert

   Article

   Kilchert et al Genome Res. 2020 Jul;30(7):1012-1026. do

   Insert

   3xHA-3xFLAG-hygR
   

   Use

   Pcr-based tagging in yeast

   Tags

   3xHA-3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRR-AR-5Z

    Plasmid

    #23058

    Depositor

    Charles Miller

    Article

    Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

    Insert

    AR (AR Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 29, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pJZC638

    Plasmid

    #62283

    Purpose

    Expresses MCP-VP64 and dCas9 in Yeast cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    MCP-VP64
    dCas9
    

    Use

    Tags

    VP64

    Expression

    Yeast

    Mutation

    Nuclease activity has been deactivated by mutatio…

    Promoter

    pAdh and pGal10

    Available since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. p415TEF cyto roGFP(iL)-Grx1(human)

    Plasmid

    #65002

    Purpose

    Yeast expression plasmid for roGFP1(iL)-Grx1; yeast codon optimized roGFP; human Grx1

    Depositor

    Tobias Dick

    Article

    Morgan et al Nat Chem Biol. 2013 Feb;9(2):119-25. doi

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Use

    Tags

    roGFP(iL)

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSUC2::GNSI (NrsR)

    Plasmid

    #121537

    Purpose

    This plasmid contains a CYC1pr driven GNSI reporter and flanking homology to SUC2. Digestion with NotI, SacI, and EcoRV, allows integration at the SUC2 locus.

    Depositor

    Alexey Merz

    Article

    Plemel et al Traffic. 2020 Nov 23. doi: 10.1111/tra.1

    Inserts

    CYC1 Promoter (CYC1 Budding Yeast)
    Nyv1 Cytosolic Domain (aa 6-230)
    Snc1 Transmembrane Domain (TMD)
    SUC2 CDS
    SUC2 terminator
    NrsR
    

    Use

    Tags

    mGFP5

    Expression

    Yeast

    Mutation

    M109I (Naturally occurring SNP) and The first 21 …

    Promoter

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. KM-CashGem-Xyl2-URA3-LINEAR

    Plasmid

    #174838

    Purpose

    pRS414 backbone containing LINEAR fragment targeting XYL2 in K. marxianus

    Depositor

    Zengyi Shao

    Article

    Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

    Insert

    Codon optimized Cas9:hGem
    

    Use

    CRISPR and Synthetic Biology

    Tags

    hGeminin tag

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    TEF1p

    Available since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. mito-yHS1-M7A

    Plasmid

    #159164

    Purpose

    Mitochondrial labile heme reporter

    Depositor

    Amit Reddi

    Article

    Martinez-Guzman et al J Cell Sci. 2020 May 20;133(10). pii: jc

    Insert

    mito-yHS1-M7A
    

    Use

    Tags

    COX4 pre-sequence

    Expression

    Yeast

    Mutation

    Mutated Met 7 of the cyt b562 module of mito-yHS1…

    Promoter

    GPD

    Available since

    Sept. 21, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pUDE731

    Plasmid

    #103008

    Purpose

    S. cerevisiae Episomal plasmid harboring Fncpf1 under the control of TDH3p

    Depositor

    Jean-Marc Daran

    Article

    Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

    Insert

    Fncpf1
    

    Use

    Tags

    3HA and NLS

    Expression

    Yeast

    Mutation

    human codon optimized

    Promoter

    TEF1

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. YL-CashGem-BAT1-URA3-LINEAR

    Plasmid

    #174837

    Purpose

    pRS414 backbone containing LINEAR fragment targeting BAT1 in Y. lipolytica

    Depositor

    Zengyi Shao

    Article

    Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

    Insert

    Codon optimized Cas9:hGem
    

    Use

    CRISPR and Synthetic Biology

    Tags

    hGeminin tag

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    GPDp

    Available since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pL90-Nat-2xHO

    Plasmid

    #139069

    Purpose

    CRISPR/Cas9 engineering of the HO endonuclease gene with nourseothricin selection

    Depositor

    Gianni Liti

    Article

    Barre et al Genome Res. 2020 Apr 10. pii: gr.253351.

    Insert

    Two guide RNAs targeting the HO endonuclease
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    TDH3

    Available since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDN-G1Tt

    Plasmid

    #44509

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

    Inserts

    PGal1-D12 promoter (GAL1 Synthetic, Budding Yeast)
    tetR
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Tags

    Expression

    Yeast

    Mutation

    Two tandem tet operators (2XtetO2) downstream of …

    Promoter

    PGal1-D12 promoter

    Available since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGBT9-Hook2

    Plasmid

    #198524

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-Hook2 fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

    Insert

    Hook2 (HOOK2 Human)
    

    Use

    Tags

    GAL4-DNA binding domain fragment

    Expression

    Yeast

    Mutation

    His488Gln substitution

    Promoter

    ADH1

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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