We narrowed to 9,187 results for: Pol;

1. Lck-FRB-ECFP(W66A)

   Plasmid

   #67902

   Purpose

   Expresses a membrane located FRB domain in mammalian cells (the ECFP is not fluorescent)

   Depositor

   Dorus Gadella

   Article

   van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

   Insert

   Lck-FRB-ECFP(W66A)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-UbC-mCherry Rab5

   Plasmid

   #115239

   Purpose

   To express in mammalian cells the endocytic protein Rab5 fused to mCherry by transfection or by generating AAV virions.

   Depositor

   Pantelis Tsoulfas

   Insert

   Rab5a (RAB5A Human, Homo sapiens)
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   Human UbC

   Available Since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-NEMO

   Plasmid

   #11966

   Depositor

   Jon Ashwell

   Article

   Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

   Insert

   NEMO (IKBKG Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   July 13, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pNK869

   Plasmid

   #219737

   Purpose

   MoClo-compatible Level 0 - promoter + 5U vector carrying promoter AtPD7

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Int J Mol Sci. 2022 Dec 6;23(23):15441.

   Insert

   promoter of serine carboxypeptidase-like gene AtSCPL30, AtPD7
   

   Use

   Synthetic Biology

   Expression

   Plant

   Promoter

   AtPD7

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. T7RNAP Cyslight V174C

   Plasmid

   #205078

   Purpose

   T7RNAP protein expression

   Depositor

   Smita Patel

   Article

   Koh et al Mol Cell. 2018 May 17;70(4):695-706.e5.

   Insert

   T7RNAP Cyslight V174C (T7p07 Bacteriophage T7)
   

   Expression

   Bacterial

   Mutation

   C125S, C347S, C510S, C515S, C530S, C723S V174C

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Ki67∆repeats-mNeonGreen-IRESpuro2

   Plasmid

   #183742

   Purpose

   Expresses Ki67∆repeats-mNeonGreen

   Depositor

   Daniel Gerlich

   Article

   Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

   Insert

   MKI67(lacking repeats) (MKI67 Human)
   

   Tags

   mNeonGreen

   Expression

   Bacterial and Mammalian

   Mutation

   MKI67(lacking repeats)

   Promoter

   CMV

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. p426 46Q GPD

   Plasmid

   #1182

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 46Q (HTT Human)
   

   Tags

   GFP

   Expression

   Yeast

   Available Since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

8. pLK69

   Plasmid

   #37258

   Depositor

   Ann Hubbard

   Article

   Kallay et al J Cell Biochem. 2006 Oct 1;99(2):647-64.

   Insert

   vang-like 2 (VANGL2 Human)
   

   Use

   Adenoviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   lacks the 4 C-term aa

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pECFP-Cdc42Q61L

   Plasmid

   #105294

   Purpose

   Constitutively active Cdc42

   Depositor

   Kenneth Yamada

   Article

   Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

   Insert

   Cdc42 (CDC42 Human)
   

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Q61L

   Available Since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. PROM5_35Sx2

    Plasmid

    #136112

    Purpose

    Strong promoter that drives expression everywhere in Mp gemmae but much weaker in the notches

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    PROM_35Sx2 (CaMV)
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. PROM5_35S

    Plasmid

    #136111

    Purpose

    Strong promoter that drives expression everywhere in Mp gemmae but much weaker in the notches

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    PROM_35S (CaMV)
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. AL12R-HSP70

    Plasmid

    #161010

    Purpose

    PP7-tagged reporter driven by the Arabidopsis HSP70 promoter

    Depositor

    Krishna Niyogi

    Article

    Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

    Insert

    PP7 and H2B-mScarlet
    

    Expression

    Plant

    Available Since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. mt SSB

    Plasmid

    #205057

    Purpose

    SSB1 protein expression

    Depositor

    Smita Patel

    Article

    Johnson et al J Biol Chem. 2023 Jan;299(1):102797. doi

    Insert

    SSB1 (SSB Human)
    

    Expression

    Bacterial

    Mutation

    WT

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. MBP-His6-TEV-MMS2

    Plasmid

    #25465

    Depositor

    Cynthia Wolberger

    Article

    Eddins et al Nat Struct Mol Biol. 2006 Oct . 13(10):9

    Insert

    MMS2 (MMS2, UBC13 Budding Yeast)
    

    Tags

    His6 and MBP

    Expression

    Bacterial

    Available Since

    July 21, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pCk1_spacer

    Plasmid

    #136071

    Purpose

    Use when making even level constructs (L2,...) if pCk1 position (position 1) has no construct assigned

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    Odd spacer
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pSV(X) C/EBPdelta

    Plasmid

    #13394

    Depositor

    Richard Schwartz

    Article

    Hu et al J Immunol. 1998 Mar 1. 160(5):2334-42.

    Insert

    C/EBPdelta (Cebpd Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. p426 25Q GPD

    Plasmid

    #1181

    Depositor

    Susan Lindquist

    Article

    Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

    Insert

    htt 25Q (HTT Human)
    

    Tags

    GFP

    Expression

    Yeast

    Available Since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

18. pBABE M33 flag

    Plasmid

    #1952

    Depositor

    Robert Kingston

    Article

    Levine et al Mol Cell Biol 2002 Sep;22(17):6070-8.

    Insert

    M33 (Cbx2 Mouse)
    

    Use

    Retroviral

    Tags

    flag

    Expression

    Mammalian

    Available Since

    March 23, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pEGFP-C1 beta-catenin Y654E

    Plasmid

    #16073

    Depositor

    Erin Schuman

    Article

    Murase et al Neuron. 2002 Jul 3. 35(1):91-105.

    Insert

    beta-catenin (CTNNB1 Chicken)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Y654E, *(see below)

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. UMG-SP-1_pET29b

    Plasmid

    #215433

    Purpose

    Expression construct for UMG-SP-1 gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    UMG-SP-1
    

    Expression

    Bacterial

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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