We narrowed to 4,631 results for: lum

1. pmTurquoise2-ER

   Plasmid

   #36204

   Purpose

   In vivo visualization of the endoplasmic reticulum (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Insert

   ER-mTurquoise2
   

   Use

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO_CalR_HaloTag9_KDEL

   Plasmid

   #175540

   Purpose

   Expression of HaloTag9 in the endoplasmid reticulum of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   CalR-HaloTag9-KDEL
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAPV-DsRed2-ER-5

   Plasmid

   #170570

   Purpose

   Expresses DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV tripeptide after signal peptide cleavage.

   Depositor

   Ke Xu

   Article

   Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

   Insert

   APV-DsRed2-ER-5
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Str-KDEL_neomycin

   Plasmid

   #65306

   Purpose

   Luminal ER hook only (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end
   

   Use

   Tags

   KDEL motif and Signal Sequence

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMT-Bip-GFP:V5:KDEL

   Plasmid

   #69917

   Purpose

   An endoplasmic reticulum (ER) marker used to visulaize the ER structure in Drosophilia S2 cells.

   Depositor

   Kenneth Irvine

   Article

   Okajima et al Science. 2005 Mar 11;307(5715):1599-603.

   Insert

   GFP
   

   Use

   Tags

   Drosophila BiP signal sequence, KDEL ER targeting…

   Expression

   Insect

   Mutation

   Promoter

   pMT

   Available since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDusk

   Plasmid

   #43795

   Purpose

   Empty backbone for blue-light-regulated expression of a gene of interest. Expression is repressed under blue illumination.

   Depositor

   Andreas Moeglich

   Article

   Ohlendorf et al J Mol Biol. 2012 Mar 2;416(4):534-42. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCerulean3-CytERM-N-17

   Plasmid

   #55412

   Purpose

   Localization: Endoplasmic Reticulum, Excitation: 433, Emission: 475

   Depositor

   Michael Davidson

   Article

   Markwardt et al PLoS One. 2011 . 6(3):e17896.

   Insert

   CytERM
   

   Use

   Tags

   mCerulean3

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMpGWB402-er-mCherry

   Plasmid

   #186288

   Purpose

   Binary vector for the expression of er-mCherry (SP-mCherry-HDEL) in plants (for Agrobacterium-mediated genetic transformation and visualization of endoplasmic reticulum)

   Depositor

   Yutaka Kodama

   Article

   Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

   Insert

   er-mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEFIRES-P-mTagBFP-KDEL

   Plasmid

   #87163

   Purpose

   Fluorescent marker of endoplasmic reticulum (ER)

   Depositor

   Elina Ikonen

   Article

   Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

   Insert

   mTagBFP-KDEL
   

   Use

   Tags

   Bip signal peptide (Homo sapiens) and mTagBFP-KDE…

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1 alpha

   Available since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. iRFP-FRB-ER

    Plasmid

    #139317

    Purpose

    endoplasmic reticulum-resident FRB for chemically induced dimerization

    Depositor

    Gerry Hammond

    Article

    Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    iRFP:MTOR(2021-2113):[GGSA]2ILNSRV:SACM1L(521-587)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMDC32-Kar2-CFP-HDEL

    Plasmid

    #96989

    Purpose

    Express endoplasmic reticulum (ER) marker in plants.

    Depositor

    Kent Chapman

    Article

    Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

    Insert

    Kar2-CFP-HDEL
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    July 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIgnaviCas9

    Plasmid

    #127595

    Purpose

    Expresses IgnaviCas9 in E. coli

    Depositor

    Stephen Quake

    Article

    Schmidt et al Proc Natl Acad Sci U S A. 2019 Oct 28. p

    Insert

    IgnaviCas9
    

    Use

    Tags

    6xHis Tag and Maltose binding protein

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRATIO3267

    Plasmid

    #141312

    Purpose

    Gateway cloning compatible dual bioluminescent ratiometric binary vector

    Depositor

    David Nelson

    Article

    Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

    Type

    Empty backbone

    Use

    Tags

    gLUC and redLUC

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S promoter

    Available since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRATIO1267

    Plasmid

    #141301

    Purpose

    Gateway cloning compatible dual bioluminescent ratiometric binary vector

    Depositor

    David Nelson

    Article

    Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

    Type

    Empty backbone

    Use

    Tags

    gLUC and redLUC

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S promoter

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pQIq_MRGS_HIS8_(27G2)_FLAG

    Plasmid

    #199616

    Purpose

    Encodes DARPin-FLAG 27G2 for bacterial expression and column purification

    Depositor

    Shiva Tyagarajan

    Article

    Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

    Insert

    DARPin-FLAG 27G2
    

    Use

    Tags

    8xHis and FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    May 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-ER-rsTagRFP

    Plasmid

    #87620

    Purpose

    Endoplasmic reticulum (ER)-targeted rsTagRFP.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    ER-rsTagRFP
    

    Use

    Tags

    N-terminal 27 amino acids from CYP450

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCherry-Cb5-IRES-puromycin-pLVx-EF1a

    Plasmid

    #177434

    Purpose

    To express mCherry fused to Cb5 endoplasmic reticulum (ER) targeting sequence. Lentiviral vector used to make cell lines expressing this ER landmark.

    Depositor

    David Andrews

    Insert

    Cb5 tail anchor (Cyb5r4 Rat)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAPV-DsRed2-FLAG-ER-5

    Plasmid

    #170622

    Purpose

    Expresses DsRed2-FLAG targeted to the ER lumen with an N-terminal APV tripeptide after signal peptide cleavage. The protein is efficiently retrieved due to exposure of KDEL.

    Depositor

    Ke Xu

    Article

    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

    Insert

    APV-DsRed2-ER-5
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-PRLSS-SH2-KDEL

    Plasmid

    #209716

    Purpose

    Mammalian expression of SH2 localized to endoplasmic reticulum

    Depositor

    Stewart Loh

    Article

    Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

    Insert

    PRLSS-SH2-KDEL
    

    Use

    Tags

    PRLSS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pQIq_MRGS_HIS8_( E3_5)_FLAG

    Plasmid

    #199612

    Purpose

    Encodes DARPin-FLAG E3_5 for bacterial expression and column purification

    Depositor

    Shiva Tyagarajan

    Article

    Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

    Insert

    DARPin-FLAG E3_5 (control)
    

    Use

    Tags

    8xHis and FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    May 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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