We narrowed to 5,631 results for: pcas

1. p458 VRER

   Plasmid

   #101728

   Purpose

   Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VRER
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   VRER (D1135V, G1218R, R1335E, and T1337R mutation…

   Promoter

   Available since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. RNF34

   Plasmid

   #119937

   Purpose

   RING finger protein 34 expression

   Depositor

   Angel de Blas

   Article

   Jin et al J Biol Chem. 2014 Oct 17;289(42):29420-3

   Insert

   RNF34 (Rnf34 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

3. p330 VQR

   Plasmid

   #101730

   Purpose

   Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VQR
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   VQR (D1135V, R1335Q, and T1337R mutations in Cas9)

   Promoter

   Available since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFPL-Cg

   Plasmid

   #61652

   Purpose

   Gateway vector for generating cyan fluorescent protein fusions in fungi

   Depositor

   Mark Farman

   Article

   Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

   Insert

   enhanced cyan fluorescent protein
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 26, 2015

   Availability

   Academic Institutions and Nonprofits only

5. PEP113E

   Plasmid

   #97399

   Purpose

   binary construct of peroxisome-targeted mCherry with sfGFP11 tag for plant expression

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

   Insert

   mCherry-sfGFP11
   

   Use

   Tags

   Peroxisome target

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. MTK234_033

   Plasmid

   #123996

   Purpose

   Encodes the spCas9 sgRNA1 hAAVS1 (site 2) gRNA expression cassette as a type 234 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   sgRNA2 -hAAVS1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. MTK234_034

   Plasmid

   #123997

   Purpose

   Encodes the spCas9 sgRNA1 hAAVS1 (site 3) gRNA expression cassette as a type 234 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   sgRNA3 -hAAVS1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRU52

   Plasmid

   #167678

   Purpose

   Final destination vector for single Gateway LR reaction

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   PcUB4-2::spCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCX-2A-2A

   Plasmid

   #74670

   Purpose

   based on pCX-C1 with F2A1/F2A2 inserted

   Depositor

   Roberto Giovannoni

   Article

   De Giorgi et al Plasmid. 2015 May;79:22-9. doi: 10.1016/

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCAGGS

   Available since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. NAX_CAGGS_CAS9_KanR neo

    Plasmid

    #167861

    Purpose

    PiggyBac compatible plasmid expressing spCas9

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDGB3_alpha1

    Plasmid

    #68228

    Purpose

    GoldenBraid level 1 Alpha plant expression vector.

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    empty vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. CALNL-RARa-VP16

    Plasmid

    #185560

    Purpose

    Cre Dependent ubiquitous expression of constitutively active Retinoic Acid Receptor alpha

    Depositor

    Connie Cepko

    Article

    Amamoto et al Elife. 2022 Mar 22;11. pii: 76389. doi:

    Insert

    RARa-VP16 (Rara Mouse)
    

    Use

    Tags

    VP16

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMZ-180

    Plasmid

    #195428

    Purpose

    Transient, constitutive expression of synZiFTR: ZF5-p65

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    pUb - 3XFLAG - NLS - ZF5-7 (ZF5) - p65 - RbGpA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMZ-181

    Plasmid

    #195429

    Purpose

    Transient, constitutive expression of synZiFTR: ZF6-p65

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    pUb - 3XFLAG - NLS - ZF6-4 (ZF6) - p65 - RbGpA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. HA-RNF34

    Plasmid

    #119938

    Purpose

    RING finger protein 34 expression (HA-tag at the N-terminus)

    Depositor

    Angel de Blas

    Article

    Jin et al J Biol Chem. 2014 Oct 17;289(42):29420-3

    Insert

    RNF34 (Rnf34 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

16. pRU51

    Plasmid

    #167677

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    PcUB4-2::spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. CP1300-ABEmax

    Plasmid

    #119810

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    ecTadA-ecTadA*(7.10)-nSpCas9 (CP1300)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. cPE

    Plasmid

    #183549

    Purpose

    Expresses compact Prime Editor in mammalian cells

    Depositor

    Wen Xue

    Article

    Zheng et al Mol Ther. 2022 Mar 2;30(3):1343-1351. do

    Insert

    SpCas9 nickase (H840A), Reverse Transcriptase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. Or1a-Gal4

    Plasmid

    #63019

    Purpose

    To express Gal4 in the pattern of the Drosophila melanogaster odorant receptor Or1a, using the promoter sequences of the gene cloned 5' of Gal4

    Depositor

    Leslie Vosshall

    Article

    Fishilevich et al Curr Biol. 2005 Dec 6;15(23):2086-96.

    Insert

    Drosophila melanogaster Or1a promoter
    

    Use

    Tags

    Drosophila ADH (aa's 1-18) fused to Yeast Ga…

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 22, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pJK03_Rho_basal_DsRed

    Plasmid

    #173490

    Purpose

    Vector backbone used to clone MPRA libraries

    Depositor

    Barak Cohen

    Article

    White et al Proc Natl Acad Sci U S A. 2013 Jul 16;11

    Insert

    Bovine Rhodopsin promoter driving DsRed fluorescent protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Bovine Rhodopsin

    Available since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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