We narrowed to 22,191 results for: his

1. pTNS1

   Plasmid

   #87801

   Purpose

   For constructing NusA-SUMO-fusion proteins with N-terminal His tag and C-terminal strep II tag using "blunt-end" cloning.

   Depositor

   Yu-Zhu Zhang

   Article

   Zhang et al Mol Biotechnol. 2017 May;59(4-5):159-167

   Type

   Empty backbone

   Use

   Tags

   6xHis and Strep II

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFBXC3H

   Plasmid

   #49050

   Purpose

   FX cloning insect cell expression vector

   Depositor

   Markus Gruetter

   Article

   Weinert et al Sci Rep. 2015 Jun 4;5:10819. doi: 10.103

   Type

   Empty backbone

   Use

   Tags

   3C protease site (PreScission site) and decaHis (…

   Expression

   Insect

   Mutation

   Promoter

   polH (polyhedrin)

   Available Since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFBXNH3

   Plasmid

   #49049

   Purpose

   FX cloning insect cell expression vector

   Depositor

   Markus Gruetter

   Article

   Weinert et al Sci Rep. 2015 Jun 4;5:10819. doi: 10.103

   Type

   Empty backbone

   Use

   Tags

   3C protease site (PreScission site) and decaHis (…

   Expression

   Insect

   Mutation

   Promoter

   polH (polyhedrin)

   Available Since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAGGS-hCD4-GCaMP2

   Plasmid

   #18932

   Depositor

   Karel Svoboda

   Article

   Mao et al PLoS ONE. 2008 . 3(3):e1796.

   Insert

   hCD4-GCaMP2 (CD4 Human)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 3, 2008

   Availability

   Academic Institutions and Nonprofits only

5. EPHA7 (3NRU)

   Plasmid

   #28126

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   EPHA7:SDC173-D03:C209131 (EPHA7 Human)
   

   Use

   Tags

   His and Honeybee melittin Signal sequence (MKFLVN…

   Expression

   Bacterial

   Mutation

   contains amino acids residues 32-204.

   Promoter

   Available Since

   Feb. 10, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

6. HIV-1 integrase W235F in pET-15b

   Plasmid

   #61681

   Purpose

   HIV-1 integrase W235F in pET-15b

   Depositor

   Robert Craigie

   Article

   Li et al EMBO J. 2006 Mar 22;25(6):1295-304. Epub

   Insert

   HIV-1 integrase W235F
   

   Use

   Tags

   His

   Expression

   Mutation

   W235F

   Promoter

   T7

   Available Since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pBC2FG

   Plasmid

   #31281

   Depositor

   Aziz Sancar

   Article

   Ye et al J Biol Chem. 2011 Jul 22;286(29):25891-9

   Insert

   Cry2 (Cry2 Mouse)
   

   Use

   Tags

   Flag and His

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Sept. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pQE30 NFAT5 DBD

   Plasmid

   #13629

   Depositor

   Anjana Rao

   Article

   Lopez-Rodriguez et al Proc Natl Acad Sci U S A. 1999 Jun 22. 9

   Insert

   NFAT5 (NFAT5 Human)
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   DNA binding domain: aa 175 to 471

   Promoter

   Available Since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pCMV-H-RAN-H2A2B

   Plasmid

   #106419

   Purpose

   H-RAN-H2A2B (RANbody H2A2B::Sm-HAtag)

   Depositor

   Joshua Sanes

   Article

   Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

   Insert

   H-RAN-H2A2B
   

   Use

   Tags

   HA, His, and Multiple HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFA1

    Plasmid

    #46789

    Purpose

    To express Fun30 in trans in budding yeast

    Depositor

    Patrick Varga-Weisz

    Article

    Neves-Costa et al PLoS One. 2009 Dec 1;4(12):e8111. doi: 1

    Insert

    FUN30 (FUN30 Budding Yeast)
    

    Use

    Tags

    V5

    Expression

    Yeast

    Mutation

    Promoter

    PGAL1

    Available Since

    Aug. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pRSETA_crE6

    Plasmid

    #172928

    Purpose

    Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 1 helix and 2 GSG linkers

    Depositor

    Arnold Boersma

    Article

    Liu et al Biophys J. 2017 May 9;112(9):1929-1939.

    Insert

    CrowdingSensorE6
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. EF2451_pET21a(+)

    Plasmid

    #13840

    Depositor

    SeWon Suh

    Article

    Kang et al Acta Crystallograph Sect F Struct Biol C

    Insert

    PPAT
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. YFP-Gamma 9-3

    Plasmid

    #36074

    Depositor

    Narasimhan Gautam

    Article

    Chisari et al PLoS One. 2009 Nov 11;4(11):e7797.

    Insert

    G protein G9(gamma 3 tail)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    HIs tag between YFP and insert

    Promoter

    CMV

    Available Since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pMMRSF1-16

    Plasmid

    #20177

    Depositor

    Hideo Iwai

    Article

    Muona et al Chembiochem. 2008 Dec 15. 9(18):2958-61.

    Insert

    src homology domain 3 (CRK Human)
    

    Use

    Tags

    His and NpuDnaEC16

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCellFree_G03 TCF25

    Plasmid

    #67114

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TCF25 (TCF25 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

16. JARID1BA

    Plasmid

    #74648

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    JARID1BA (KDM5B Human)
    

    Use

    Baculovirus

    Tags

    His-6-TEV

    Expression

    Mutation

    Leu101-(Gly)4-Ala374 deletion mutant

    Promoter

    PH

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pcpmAmetrine

    Plasmid

    #42170

    Depositor

    Robert Campbell

    Article

    Ding et al Anal Chem. 2011 Dec 15;83(24):9687-93. d

    Insert

    cpmAmetrine173
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available Since

    Feb. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pCellFree_G03 TFEB

    Plasmid

    #67097

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TFEB (TFEB Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pCMV-M-RAN-RFP6

    Plasmid

    #106428

    Purpose

    M-RAN-RFP6 (RANbody RFP6::Sm-MYCtag)

    Depositor

    Joshua Sanes

    Article

    Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

    Insert

    M-RAN-RFP6
    

    Use

    Tags

    HA, His, and Multiple MYC

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-21a(+)-DR RecR

    Plasmid

    #12617

    Depositor

    SeWon Suh

    Article

    Lee et al EMBO J. 2004 May 19. 23(10):2029-38.

    Insert

    Deinococcus radiodurans RecR (recR Deinococcus radiodurans)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDEST17-hOPTN-D474N

    Plasmid

    #23055

    Depositor

    Jon Ashwell

    Article

    Zhu et al Curr Biol. 2007 Aug 21. 17(16):1438-43.

    Insert

    Optineurin (OPTN Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    D474N

    Promoter

    Available Since

    March 3, 2010

    Availability

    Academic Institutions and Nonprofits only

22. SAPDF-pET21a(+)

    Plasmid

    #12649

    Depositor

    SeWon Suh

    Article

    Yoon et al Proteins. 2004 Nov 15. 57(3):639-42.

    Insert

    SA Peptide deformylase
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJK_proK6_eGFP

    Plasmid

    #59850

    Purpose

    Constitutively expresses eGFP with proK6 promoter

    Depositor

    Timothy Whitehead

    Article

    Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

    Insert

    Enhanced Green Fluorescent Protein
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    S2R,S65G,S72A

    Promoter

    proK6

    Available Since

    Oct. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. wt Cus2p

    Plasmid

    #73046

    Purpose

    Wild-type S. cerevisiae Cus2p gene for recombinant expression and crystallization

    Depositor

    Aaron Hoskins

    Article

    Rodgers et al RNA. 2015 Dec 2.

    Insert

    CUS2p (CUS2 Budding Yeast)
    

    Use

    Tags

    6x His tag fused to TEV cleavage site

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    T7

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET-28a-CylA

    Plasmid

    #48198

    Purpose

    Expresses CylA (fatty acyl-AMP ligase) in E. coli

    Depositor

    Emily Balskus

    Article

    Nakamura et al J Am Chem Soc. 2012 Nov 14;134(45):18518

    Insert

    CylA
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBN40

    Plasmid

    #86715

    Purpose

    C. elegans injection marker

    Depositor

    Peter Askjaer

    Article

    Morales-Martinez et al J Cell Sci. 2015 Mar 15;128(6):1090-6. d

    Insert

    Plmn-1::gfp::his-58
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET28-p53 (Dog 1-77)

    Plasmid

    #62060

    Purpose

    Expression of Dog p53 (residues 1-77) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Daughdrill et al PLoS One. 2011;6(12):e29207. doi: 10.137

    Insert

    p53
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-77

    Promoter

    T7

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

28. pBbA5k-EPL84

    Plasmid

    #45431

    Depositor

    Aindrila Mukhopadhyay

    Article

    Dunlop et al Mol Syst Biol. 2011 May 10;7:487. doi: 1

    Insert

    BP_2076
    

    Use

    Synthetic Biology

    Tags

    His

    Expression

    Mutation

    Promoter

    LacUV5

    Available Since

    Jan. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET29b-irp9 WT

    Plasmid

    #87105

    Purpose

    expresses Irp9 WT, the salicylate synthase from Yersinia enterocolitica

    Depositor

    Audrey Lamb

    Article

    Meneely et al Arch Biochem Biophys. 2013 Nov 1;539(1):

    Insert

    irp9 WT
    

    Use

    Tags

    his-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Sus1/PET32a

    Plasmid

    #26963

    Depositor

    Cynthia Wolberger

    Article

    Samara et al Science. 2010 May 21. 328(5981):1025-9.

    Insert

    Sus1 (SUS1 Budding Yeast)
    

    Use

    Tags

    His6 and Trx

    Expression

    Bacterial

    Mutation

    with TEV site to cleave the N-terminal his+Trx tag

    Promoter

    Available Since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

31. HS-H3.3A d436-GFP

    Plasmid

    #8697

    Depositor

    Kami Ahmad

    Article

    Ahmad et al Mol Cell 2002 Jun;9(6):1191-200.

    Insert

    H3.3A truncated (His3.3A Fly)
    

    Use

    Tags

    GFP

    Expression

    Insect

    Mutation

    Deletion of amino acids 4-36.

    Promoter

    Available Since

    June 30, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBbA5k-EPL98

    Plasmid

    #45436

    Depositor

    Aindrila Mukhopadhyay

    Article

    Dunlop et al Mol Syst Biol. 2011 May 10;7:487. doi: 1

    Insert

    acrB, PSHAb_0324
    

    Use

    Synthetic Biology

    Tags

    His

    Expression

    Mutation

    Promoter

    LacUV5

    Available Since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Axin GID 1-2/pET29b

    Plasmid

    #16307

    Depositor

    Peter Klein

    Article

    Hedgepeth et al Mol Cell Biol. 1999 Oct . 19(10):7147-57

    Insert

    Axin GID 1-2 (axin1.L Frog)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Amino acids 320-429

    Promoter

    Available Since

    Dec. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

34. pJK_proK17_amiE

    Plasmid

    #59837

    Purpose

    Constitutively expresses AmiE with proK17 promoter

    Depositor

    Timothy Whitehead

    Article

    Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

    Insert

    ALIPHATIC AMIDASE (amiE )
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    proK17

    Available Since

    Oct. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. AKAP13

    Plasmid

    #73230

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    AKAP13 (AKAP13 Human)
    

    Use

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    pfam00621:RhoGEF 2002-2193, pfam00169:PH 2236-2336

    Promoter

    T7

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

36. pCri-8a-Strep

    Plasmid

    #61340

    Purpose

    Protein expression.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

    Insert

    Green fluorescent protein
    

    Use

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

37. pCellFree_G03 TFDP2

    Plasmid

    #67088

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TFDP2 (TFDP2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

38. pBbA5k-EPL11

    Plasmid

    #45403

    Depositor

    Aindrila Mukhopadhyay

    Article

    Dunlop et al Mol Syst Biol. 2011 May 10;7:487. doi: 1

    Insert

    ttgB, PP_1385
    

    Use

    Synthetic Biology

    Tags

    His

    Expression

    Mutation

    Promoter

    LacUV5

    Available Since

    June 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET-21a(+) SurE D9A

    Plasmid

    #12686

    Depositor

    SeWon Suh

    Article

    Lee et al Nat Struct Biol. 2001 Sep . 8(9):789-94.

    Insert

    SurE
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    changed Aspartate 9 to Alanine

    Promoter

    Available Since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

40. pJK_pro6_eGFP

    Plasmid

    #59851

    Purpose

    Constitutively expresses eGFP with pro6 promoter

    Depositor

    Timothy Whitehead

    Article

    Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

    Insert

    Enhanced Green Fluorescent Protein
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    S2R,S65G,S72A

    Promoter

    pro6

    Available Since

    Oct. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge