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pCAG-c-Myc NLS-FKBP-Venus N-T2A-mRFP

Plasmid

#100979

Purpose

Encodes half of a protein delivery sensor ( NLS-FKBP-VN-T2A-RFP) for expression in mammalian cells

Depositor

Heinrich Leonhardt

Article

Chiu et al Nucleus. 2018 Sep 14. doi: 10.1080/19491

Insert

c-Myc NLS-FKBP-Venus N-T2A-mRFP

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSIF-H1-hpolb-copGFP

Plasmid

#23256

Depositor

Robert Sobol

Article

Tang et al Mol Cancer Res. 2010 Jan;8(1):67-79. doi

Insert

DNA polymerase beta shRNA

Use

Lentiviral and RNAi

Tags

GFP

Available Since

May 27, 2010

Availability

Academic Institutions and Nonprofits only
pRS315-PGK-SEIPIN1

Plasmid

#96986

Purpose

Express GFP-tagged Arabidopsis SEIPIN1 gene in yeast cells.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

SEIPIN1 (AT5G16460 Mustard Weed)

Expression

Yeast

Promoter

Phosphoglycerate Kinase (PGK) promoter

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pNB330

Plasmid

#60147

Purpose

Disintegrator plasmid encoding MET17 promoter driven NanoLuc (Promega) with nuclear localization signal (NLS)

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

NLuc

Tags

Nuclear localization signal (NLS)

Expression

Yeast

Promoter

MET17pr

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pMSCV-puro-5A, 6A mMCL1-OM

Plasmid

#45818

Depositor

Joseph Opferman

Article

Perciavalle et al Nat Cell Biol. 2012 Apr 29;14(6):575-83.

Insert

5A, 6A mMCL-1-OM

Expression

Mammalian

Mutation

Changed Arginine to Alanine at 5th and 6th amino …

Available Since

July 24, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-IP3KC

Plasmid

#32527

Depositor

Christophe Erneux

Article

Dewaste et al Biochem J. 2000 Dec 1;352 Pt 2:343-51.

Insert

IP3KC (ITPKC Human)

Tags

6xHIS

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only
pIIS18-SapI

Plasmid

#128599

Purpose

Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

Depositor

Tohru Suzuki

Article

Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

Type

Empty backbone

Use

Unspecified

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pIIS18-BsmBI

Plasmid

#128648

Purpose

Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

Depositor

Tohru Suzuki

Article

Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

Type

Empty backbone

Use

Unspecified

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3-Xl-VSP1-GFP

Plasmid

#51883

Purpose

expression of Xl-VSP1 -GFP fusion in Xenopus oocytes or mammalian cells

Depositor

Laurinda Jaffe

Article

Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

Insert

VSP1 (tpte2 Frog)

Use

Xenopus oocyte expression

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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LC3-mEYFP

Plasmid

#182865

Purpose

Autophagy Marker

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

rat LC3 (Anxa3 Rat)

Expression

Mammalian

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pGL2 enhancer- F1 LUC

Plasmid

#22426

Depositor

William Sessa

Article

Zhang et al J Biol Chem. 1995 Jun 23. 270(25):15320-

Insert

eNOS promotor F1 (NOS3 Human)

Use

Luciferase

Tags

Luciferase reporter gene

Expression

Mammalian

Available Since

Dec. 8, 2009

Availability

Academic Institutions and Nonprofits only
pGEX-6p-1 Sesn1b

Plasmid

#61874

Purpose

bacterial expression of a GST-Sesn1b (mouse)

Depositor

Ming Li

Article

Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

Insert

Sesn1 (Sesn1 Mouse)

Tags

GST

Expression

Bacterial

Mutation

none

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only
pCMV5 KSR1 S392A

Plasmid

#25976

Depositor

Rob Lewis

Article

Brennan et al J Biol Chem. 2002 Feb 15. 277(7):5369-77

Insert

Kinase suppressor of Ras 1 (Ksr1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

changed serine 392 to alanine

Available Since

Sept. 15, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Gα13(EE)-YFP

Plasmid

#171374

Purpose

Plasmid encoding Gα13 internally-tagged with the Citrine variant of YFP in the b/c loop

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Ga13(EE)-YFP

Tags

Glu-Glu

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 17, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pMpGWB224

Plasmid

#68615

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Triple repeats of Citrine (3xCitrine)

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pTS2163

Plasmid

#169632

Purpose

Tier-3 vector for CRISPR-mediated stable integration into the AAVS1 locus of the tetON-system (OTetO7-PCMVmin-SEAP-p2A-iRFP670-pA::PmPGK1-rtTA-pA::A3-pA)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

OTetO7-PCMVmin-SEAP-iRFP670::PmPGK1-rtTA-pA::A3

Expression

Mammalian

Promoter

tetO7-PhCMVmin / PPGK

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pLJM1 Flag-Sesn2

Plasmid

#61871

Purpose

Lentivirial expression of Flag-Sesn2 (mouse)

Depositor

Ming Li

Article

Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

Insert

Sesn2 (Sesn2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Feb. 4, 2015

Availability

Academic Institutions and Nonprofits only
pMDC43-SEIPIN3

Plasmid

#96978

Purpose

Express GFP-tagged Arabidopsis SEIPIN3 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

SEIPIN3 (AT2G34380 Mustard Weed)

Tags

GFP

Expression

Plant

Promoter

35S

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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XE28 XBC 40

Plasmid

#16842

Depositor

Randall Moon

Article

Yost et al Genes Dev. 1996 Jun 15. 10(12):1443-54.

Insert

beta-catenin-myc (ctnnb1.L Frog)

Use

Xenopus expression

Tags

6x myc

Available Since

May 22, 2009

Availability

Academic Institutions and Nonprofits only
pAAV-HDR-sphys-3XHA

Plasmid

#163690

Purpose

plasmid for homologous recombination-mediated insertion of triple HA tag into synaptophysin gene

Depositor

Bernardo Sabatini

Article

Chantranupong et al Elife. 2020 Oct 12;9. pii: 59699. doi: 1

Insert

triple HA tag insertion into endogenous mouse synaptophysin gene

Use

AAV

Tags

triple HA tag

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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