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pCMV4-p100SS/AA (SS866/870AA)

Plasmid

#27555

Depositor

Shao-Cong Sun

Article

Xiao et al EMBO J. 2001 Dec 3. 20(23):6805-15.

Insert

p100 (NFKB2 Human)

Expression

Mammalian

Mutation

Serines 866 and 870 mutated to Alanines

Available Since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only
pGBT-R16

Plasmid

#31827

Depositor

Stephen Ekker

Article

Clark et al Nat Methods. 2011 Jun;8(6):506-15. Epub

Insert

mini Tol2 ITR

Use

Cre/Lox; Gene trap

Tags

mRFP

Mutation

mRFP lacks start codon

Promoter

beta Actin

Available Since

Sept. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pKD280

Plasmid

#125071

Purpose

Expression of SO_4387 under Ptac

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

SO_4387 (SO_4387 )
lacI (lacI )

Use

Synthetic Biology

Expression

Bacterial

Mutation

S286L- Please see depositor comments below

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-MCPIP1(150-599)

Plasmid

#90108

Purpose

Mammalian expression of Flag-MCPIP1(150-599)

Depositor

Mingui Fu

Article

Liang et al J Exp Med. 2010 Dec 20;207(13):2959-73.

Insert

MCPIP1 (ZC3H12A Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pEasyG1_zeo

Plasmid

#184910

Purpose

Template to generate via PCR a single gRNA for expression in S. cerevisiae

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-fCAMK-GFP

Plasmid

#22917

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

fugu α-calcium/calmodulin-dependent protein kinase II promoter driving GFP

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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OVA-miSFIT-8-G

Plasmid

#124675

Purpose

Tuning expression of cytosolic Ovalbumin coupled to EGFP

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

Cyto-Ovalbumin

Expression

Mammalian

Mutation

Truncated OVA for cytoplasmic expression

Promoter

minimal CMV

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 Orf7b

Plasmid

#158376

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 Orf7b (ORF7b )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-SARS-CoV-2 NSP15

Plasmid

#158366

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP15 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available Since

Nov. 10, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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L_NES 67kDa

Plasmid

#201357

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

Adeno_NES-EGFP-6PrA

Expression

Mammalian

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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M_NLS 67kDa

Plasmid

#201351

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

(NLS)SV40A5-EGFP-6PrA

Expression

Mammalian

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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H_NES 67kDa

Plasmid

#201350

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

HIV_NES-EGFP-6PrA

Expression

Mammalian

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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M_NES 67kDa

Plasmid

#201356

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

MAPK_NES-EGFP-6PrA

Expression

Mammalian

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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Flag-MCPIP1(81-599)

Plasmid

#90107

Purpose

Mammalian expression of Flag-MCPIP1(81-599)

Depositor

Mingui Fu

Article

Liang et al J Exp Med. 2010 Dec 20;207(13):2959-73.

Insert

MCPIP1 (ZC3H12A Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 NSP7

Plasmid

#158358

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP7 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-CAG-EGFPnls barcode-206-SV40 polyA

Plasmid

#190875

Purpose

For barcoded retrograde labeling

Depositor

Yuejun Chen

Article

Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

Insert

EGFPnls-barcode206

Use

AAV

Mutation

WT

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag RagB

Plasmid

#112755

Purpose

express Flag-RagB

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-RagB (RRAGB Human)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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K8.1Pr-pGL4.16

Plasmid

#131037

Purpose

Firefly Luciferase reporter for K8.1 promoter from KSHV

Depositor

Britt Glaunsinger

Article

Nandakumar et al PLoS Pathog. 2019 May 16;15(5):e1007774.

Insert

KSHV K8.1 100 bp promoter

Use

Luciferase

Expression

Mammalian

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pGP8GF-AtTIR1

Plasmid

#188427

Purpose

Arabidopsis Nuclear Auxin Signaling Receptor TIR1

Depositor

Britney Moss

Article

Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

Insert

Auxin Signaling Receptor TIR1 (TIR1 Mustard Weed)

Expression

Yeast

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pPA-RL-FL

Plasmid

#78863

Purpose

DULIP Tandem Construct used for data normalization

Depositor

Erich Wanker

Article

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Insert

Firefly

Use

Expression vector

Tags

Protein A, Renilla luciferase

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2016

Availability

Academic Institutions and Nonprofits only

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