We narrowed to 7,639 results for: CCH

1. pBS-SKII-3XHA-HALO-Kanamycin

   Plasmid

   #188933

   Purpose

   Integration cassette to create C-terminal fusions for fluorescent tagging

   Depositor

   Stephen Buratowski

   Article

   Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

   Insert

   3XHA-HALO-Kanamycin
   

   Expression

   Yeast

   Available Since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX2TK-Num1-PH

   Plasmid

   #71369

   Purpose

   bacterial expression of PH domain for phosphoinositide binding assay

   Depositor

   Mark Lemmon

   Article

   Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

   Insert

   Num1-PH
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Pleckstrin homology (PH) domain; aa2563-2692

   Promoter

   tac

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

3. EC2_7_dCas9_HC_sgRNA

   Plasmid

   #163711

   Purpose

   High copy plasmid with dCas9 and sgRNA expression cassettes

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Insert

   dCas9
   

   Expression

   Yeast

   Mutation

   Point mutations D10A and H840A

   Promoter

   ScTEF1

   Available Since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIM700

   Plasmid

   #74639

   Purpose

   alias pFA6a-yTagRFP-T-natNT2 - vector for C-terminal tagging of yeast genes with yeast-adapted TagRFP-T and nourseothricin selection marker

   Depositor

   Jiří Hašek

   Article

   Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

   Insert

   yTagRFP-T
   

   Expression

   Yeast

   Promoter

   none

   Available Since

   June 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. M3938 trp1::ADE2 Disruptor Converter

   Plasmid

   #51673

   Purpose

   Yeast marker swap plasmid for converting TRP1 to ADE2

   Depositor

   David Stillman

   Article

   Voth et al Yeast. 2003 Aug;20(11):985-93.

   Insert

   ADE2 (ADE2 Budding Yeast)
   

   Use

   Yeast marker swap

   Expression

   Yeast

   Promoter

   ADE2

   Available Since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMAL498

   Plasmid

   #169154

   Purpose

   Encodes OptoQ-INVRT4

   Depositor

   Jose Avalos

   Article

   Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

   Insert

   VP16-EL222
   

   Tags

   SV40 NLS

   Expression

   Yeast

   Available Since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHES822

   Plasmid

   #89193

   Purpose

   Expresses YFP under control of synthetic promoter containing Zif268 binding sites.

   Depositor

   Hana El-Samad

   Article

   Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

   Insert

   pZ-YFP
   

   Expression

   Yeast

   Promoter

   pZ

   Available Since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRS426-GFP-Osh1-PH

   Plasmid

   #71372

   Purpose

   For analysis of PH domain localization in yeast

   Depositor

   Mark Lemmon

   Article

   Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

   Insert

   Osh1-PH
   

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Pleckstrin homology (PH) domain; aa267-388

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pGEX2TK-Skm1-PH

   Plasmid

   #71371

   Purpose

   bacterial expression of PH domain for phosphoinositide binding assay

   Depositor

   Mark Lemmon

   Article

   Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

   Insert

   Skm1-PH
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Pleckstrin homology (PH) domain; aa1-132

   Promoter

   tac

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pET15b I-SceI (Ctermhis -MH)

    Plasmid

    #195613

    Purpose

    Expresses the wild-type I-SceI homing endonuclease from the T7 promoter

    Depositor

    Frederick Gimble

    Article

    Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.

    Insert

    I-SceI
    

    Expression

    Bacterial

    Mutation

    None

    Promoter

    T7

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX4T1-GST-eIF4G1-His6

    Plasmid

    #37231

    Depositor

    Jon Lorsch

    Article

    Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

    Insert

    TIF4631 (TIF4631 Budding Yeast)
    

    Tags

    GST and His6

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Sept. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

12. Dcp2 FL

    Plasmid

    #163574

    Purpose

    express wild type Dcp2 at endogenous level in S.cerevisiae

    Depositor

    Roy Parker

    Article

    Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

    Insert

    Dcp2 (DCP2 Budding Yeast)
    

    Tags

    GFP

    Expression

    Yeast

    Promoter

    DCP2 promoter

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. p415 Glo3

    Plasmid

    #112651

    Purpose

    Expression S.cerevisiae

    Depositor

    Blanche Schwappach

    Article

    Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

    Insert

    Glo3
    

    Expression

    Yeast

    Promoter

    Met25

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. GST-TEVcs-FIP200-MBP

    Plasmid

    #171410

    Purpose

    For the purifcation of human ULK1 complex

    Depositor

    James Hurley

    Article

    Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

    Insert

    FIP200 (RB1CC1 Synthetic)
    

    Expression

    Mammalian

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBS_KS_attB2_SA(0)_T2AGAL4

    Plasmid

    #125211

    Purpose

    Artificial phase 0 exon to include a spliced T2A-GAL4 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

    Depositor

    Herman Dierick

    Article

    Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

    Insert

    GAL4
    

    Use

    Other

    Tags

    T2A

    Available Since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. Sc eRF1 (WT)

    Plasmid

    #175413

    Purpose

    Encodes His-MBP-TEV-WT eRF1

    Depositor

    Joseph Puglisi

    Article

    Lawson et al Science. 2021 Aug 20;373(6557):876-882.

    Insert

    eRF1 (SUP45 Budding Yeast)
    

    Tags

    His-MBP-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Dhh1-EGFP

    Plasmid

    #201418

    Purpose

    Bacterial protein expression of Dhh1

    Depositor

    Michael Rosen

    Article

    Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

    Insert

    Dhh1 (DHH1 Budding Yeast)
    

    Tags

    EGFP, TEV, 6xHis and MBP, TEV

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRS423-TyrSgH

    Plasmid

    #163973

    Purpose

    Helper plasmid for cloning gRNAs under the control of the tRNA(Tyr) promoter

    Depositor

    Jiazhang Lian

    Article

    Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Yeast

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMAL724

    Plasmid

    #169151

    Purpose

    Encodes OptoQ-AMP5

    Depositor

    Jose Avalos

    Article

    Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

    Insert

    VP16-EL222
    

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    A79Q

    Available Since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYES2.1-cerMET6

    Plasmid

    #107438

    Purpose

    Overexpression of MET fusion protein in S. cerevisiae

    Depositor

    Clare O'Connor

    Article

    Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

    Insert

    MET6 (MET6 Budding Yeast)
    

    Tags

    V5-His

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    April 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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