We narrowed to 7,040 results for: SIM

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pCfB2988

Plasmid

#63638

Purpose

pCfB2988 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.URA3. Additionally it contains a USER cassette.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Promoter

none

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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RNF146-c005

Plasmid

#173091

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

RNF146 (RNF146 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

R99-L183, codon optimized

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCI_AFF-1-FLAG_H2B-RFP

Plasmid

#240506

Purpose

Cell fusion assays

Depositor

Benjamin Podbilewicz

Article

Moi et al Nat Commun. 2022 Jul 6;13(1):3880. doi:

Insert

AFF-1

Tags

H2B-RFP

Expression

Mammalian

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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GDAP2-c005

Plasmid

#173110

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

GDAP2 (GDAP2 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

M1-N231

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pDuRCC-N

Plasmid

#81194

Purpose

Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin Resistance

Depositor

Eckhard Boles

Article

Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

Inserts

Cas9
empty gRNA cassette
empty gRNA cassette

Use

CRISPR

Tags

NTS

Expression

Yeast

Mutation

Please view depositor comments below and WT - cod…

Promoter

ROX3 and SNP52p

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pJL057

Plasmid

#177169

Purpose

Expresses a nuclease dead MRE11 in human cells

Depositor

Brittany Adamson

Article

Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

Insert

MRE11 H129N (MRE11 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

nuclease-inactivating mutation (H129N)

Promoter

EF1a

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pQStrep2-PREI3

Plasmid

#31592

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

preimplantation protein 3 (MOB4 Human)

Tags

His and StrepII-tag

Expression

Bacterial

Available Since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

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pCfB2796

Plasmid

#63641

Purpose

pCfB2796 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.URA3. Additionally it contains a USER cassette.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Promoter

none

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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L2_UBE2:eG-Lt-CsA

Plasmid

#136134

Purpose

Plasmid to transform Mp with constitutive promoter marking cell outline with eGFP. HygR

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-35S:HygR p5-MpUBE2:eGFP-Lti6b

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ1410 - [1-2] ENTR - Fluor - mCherry(PATCs, NLS(2), no_atg, no_stop)

Plasmid

#159859

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - mCherry(PATCs, NLS(2), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pICH47802-p35S::tdTomato-ER::tNOS

Plasmid

#160220

Purpose

tdTomato fluorescent protein-ER localization, 35 promoter, tNOS terminator, transcriptional unit, inserted at the MoClo toolkit level 1 acceptor position 1 reverse

Depositor

Matias Kirst

Article

Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

Insert

p2x35S::TdTomato-ER::tNOS

Use

Synthetic Biology; Moclo toolkit level 1 acceptor…

Expression

Plant

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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3×Cherry Dam AID Smash

Plasmid

#227374

Purpose

For inducible expression and cytosolic localization of Dam

Depositor

David Pellman

Article

Papathanasiou et al Nature. 2023 Jul;619(7968):184-192. doi:

Insert

Dam DNA-adenine methyltransferase

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pMDJ17 - pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]

Plasmid

#159803

Purpose

Expression construct with ubiquitous expression to test fluorescence level on microscope

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pNBA001

Plasmid

#155323

Purpose

Binary vector for expression of PULSE-controlled EFR-GFP

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

LB_(etr)8-(C120)5-PhCMVmin-EFR-GFP-T35S_RB

Use

Synthetic Biology; Eukaryotic expression

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pNBA002

Plasmid

#155324

Purpose

Binary vector for expression of PULSE-controlled GBP-mCherry

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

LB_(etr)8-(C120)5-PhCMVmin-GBP-mCherry-T35S_RB

Use

Synthetic Biology; Eukaryotic expression

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pDest-303

Plasmid

#159678

Purpose

Gateway destination vector for mammalian protein expression

Depositor

Dominic Esposito

Article

Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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ARH1-c003

Plasmid

#173093

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

ADPRH (ADPRH Human)

Tags

mCerulean

Expression

Bacterial

Mutation

E2-L357, codon optimized

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-NPY-ClopHensor

Plasmid

#25939

Depositor

Daniele Arosio

Article

Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

Insert

NPY-ClopHensor (NPY Aq. Victoria and Discosoma sp)

Tags

NPY and Strep-II

Expression

Mammalian

Mutation

Neuropeptide Y localization signal

Available Since

Sept. 13, 2010

Availability

Academic Institutions and Nonprofits only

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pLV-SI-121

Plasmid

#131126

Purpose

A-to-G base editing reporter

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

mutant EGFP

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

CMV promoter

Available Since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pROF417

Plasmid

#155335

Purpose

Constitutive expression LFY-VP16-2A-RLuc in plants

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

PCaMV35S-LFY-VP16-NLS-2A-RLuc-Tnos

Use

Luciferase and Synthetic Biology

Expression

Plant

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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