We narrowed to 18,890 results for: Tec

1. ABE8e

   Plasmid

   #138489

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(8e)-nSpCas9
   

   Expression

   Mammalian

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO-shBRCA1 #1

   Plasmid

   #44594

   Depositor

   Eros Lazzerini Denchi

   Article

   Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature1

   Insert

   BRCA1 (Brca1 Mouse)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pLKO-shBRCA1 #2

   Plasmid

   #44595

   Depositor

   Eros Lazzerini Denchi

   Article

   Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature1

   Insert

   BRCA1 (Brca1 Mouse)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pCS2+cPLA2-mKate2

   Plasmid

   #162068

   Purpose

   Used to provide a steady-state readout of cPLA2 activation in live cells. Amino-terminally fused to mKate2 for red fluorescence.

   Depositor

   Philipp Niethammer

   Article

   Enyedi et al Nat Cell Biol. 2013 Sep;15(9):1123-30. d

   Insert

   cytosolic phospholipase a2 (pla2g4aa Zebrafish)
   

   Use

   Avian, xenopus, zebrafish

   Tags

   mKate2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. YSBE 516 : pNEO_4xFLAG

   Plasmid

   #92082

   Purpose

   Plasmid for tagging proteins in Cryptococcus neoformans; 4xFLAG tag, Neo selection

   Depositor

   Yong-Sun Bahn

   Article

   So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

   Insert

   4xFlag
   

   Use

   tagging proteins in cryptococcus neoforman

   Available Since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFYF1320 EGFP Site#1

   Plasmid

   #47511

   Purpose

   human gRNA expression vector targeting EGFP

   Depositor

   Keith Joung

   Article

   Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

   Insert

   gRNA-EGFP site 1
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. dead Sa Cas9

   Plasmid

   #138162

   Purpose

   For orthogonal R loop assay (use with Sp-derived base editor, Sp gRNA, and Sa gRNA for assay)

   Depositor

   David Liu

   Article

   Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

   Insert

   dSaCas9
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   D10A + N580A

   Promoter

   CMV

   Available Since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pICH86966::AtU6p::sgRNA_PDS

   Plasmid

   #46966

   Purpose

   Expresses an sgRNA targeting the PDS gene in Nicotiana benthamiana from the Arabidopsis U6 promoter

   Depositor

   Sophien Kamoun

   Article

   Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

   Insert

   AtU6p::sgRNA_PDS
   

   Use

   CRISPR; Plant expression

   Available Since

   Aug. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. TurboID-His6_pET21a

   Plasmid

   #107177

   Purpose

   expresses His6-tagged TurboID in bacteria

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID (BirA mutant)
   

   Expression

   Bacterial

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pICSL01009::AtU6p

    Plasmid

    #46968

    Purpose

    Encodes the Arabidopsis U6 promoter in a level 0 vector (Weber et al. PLoS One 6:e16765, 2011). It is used to place an sgRNA uder the Arabidopsis U6 promoter.

    Depositor

    Sophien Kamoun

    Article

    Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

    Insert

    AtU6p
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Aug. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(+)-PKI-GFP

    Plasmid

    #118482

    Purpose

    GFP-tagged Protein Kinase A inhibitor peptide.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    PKI-GFP
    

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pH-ePPE

    Plasmid

    #183097

    Purpose

    For plant prime editing in rice plants

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2022 Mar 24. doi: 10.103

    Insert

    Nls-nCas9-NC-nls-MLV-Nls
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D10A in SpCas9

    Available Since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBT120b

    Plasmid

    #122604

    Purpose

    BE PACE selection, express gIII, luxAB and degron-GTCCA guide RNA

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    gIII, luxAB, guide RNA
    

    Use

    Synthetic Biology

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDisplay-GRAB_ATP1.0-IRES-mCherry-CAAX

    Plasmid

    #167582

    Purpose

    Express the ATP sensor GRAB_ATP1.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

    Insert

    ATP sensor GRAB_ATP1.0 (P2RY1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro

    Plasmid

    #138152

    Purpose

    Expresses eGFP in mammalian cells, contains a 3' MCS. Localized to the nucleus

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    eGFP-MCS
    

    Use

    Lentiviral

    Tags

    2PEST AND NLS

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFastBac His6 MBP Asn10 TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-C)

    Plasmid

    #55220

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Tags

    His6 and MBP, Asn10

    Expression

    Insect

    Available Since

    June 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. HPRT

    Plasmid

    #22884

    Depositor

    James Thomson

    Article

    Zwaka et al Nat Biotechnol. 2003 Mar . 21(3):319-21.

    Insert

    hypoxanthine phosphoribosyltransferase-1 (HPRT1 Human)
    

    Use

    Cre/Lox; Homologous recombination

    Mutation

    PGK, NeoR, and BGH polyA are between the two geno…

    Available Since

    May 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. v3em-Nterm-PE2max

    Plasmid

    #198734

    Purpose

    AAV genome encoding N-terminal PE2max

    Depositor

    David Liu

    Article

    Davis et al Nat Biotechnol. 2023 May 4. doi: 10.1038

    Insert

    Nterm PE2max-NpuN
    

    Use

    AAV

    Promoter

    Cbh

    Available Since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pU6-PspCas13b-gRNA-Actb1216

    Plasmid

    #155368

    Purpose

    PspCas13b guide RNA positioned 8bp 5' of Actb 1216 for targeted m6A RNA methylation

    Depositor

    David Liu

    Article

    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

    Insert

    PspCas13b gRNA targeting Actb 1216
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJRH-1187 VSVGmut

    Plasmid

    #201913

    Purpose

    Expresses a VSV-G variant (K47Q R354A) defective for LDL-R receptor binding (CAG promoter)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    VSV-G (K47Q, R354A)
    

    Expression

    Mammalian

    Mutation

    K47Q, R354A

    Promoter

    CAG

    Available Since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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