We narrowed to 11,993 results for: pcdna

1. pcDNA3puro-mGFP-FXR1a-sh5Resistant

   Plasmid

   #225448

   Purpose

   Express mEGFP-fusion protein of FXR1 isoform a with shRNA5-resistant silent mutation

   Depositor

   Christine Mayr

   Article

   Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

   Insert

   FXR1 isoform a (FXR1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-GFP-EphrinB3-Flag

   Plasmid

   #155012

   Purpose

   Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EphrinB3 from pcDNA3.1

   Depositor

   Sinisa Urban

   Article

   Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

   Insert

   EphrinB3 (EPHB3 Human)
   

   Use

   Tags

   Flag, GFP, and signal peptide (residues 1 to 32) …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1/V5-His-TOPO-mir17-92

   Plasmid

   #21109

   Depositor

   Joshua Mendell

   Article

   O'Donnell et al Nature. 2005 Jun 9. 435(7043):839-43.

   Insert

   miR-17-92 cluster (MIR17 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1his p66shc S36A (corrected)

   Plasmid

   #32575

   Depositor

   Toren Finkel

   Article

   Nemoto et al Science. 2002 Mar 29. 295(5564):2450-2.

   Insert

   p66shc (SHC1 Human)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   S36A

   Promoter

   CMV

   Available Since

   Oct. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5/FRT/TO GFP DNAJA1

   Plasmid

   #19491

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   DNAJA1 (DNAJA1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-dCas9-dMQ1-EGFP

   Plasmid

   #89637

   Purpose

   Targeted CpG methylation

   Depositor

   Margaret Goodell

   Article

   Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

   Insert

   site-specific DNA-methyltransferase SssI
   

   Use

   CRISPR

   Tags

   3*FLAG, 6*His, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   C141S, TGC-->TCC; S317A, AGC-->GCC

   Promoter

   CMV

   Available Since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-FLAG-MTOR-V2006I

   Plasmid

   #69012

   Purpose

   activating MTOR mutation

   Depositor

   David Sabatini

   Article

   Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

   Insert

   MTOR (MTOR Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   V2006I

   Promoter

   CMV

   Available Since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-Flag-Hsp40-S171A

   Plasmid

   #84999

   Purpose

   Hsp40/DnaJB1 expression plasmid

   Depositor

   Ugo Moens

   Insert

   Hsp40 (DNAJB1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Serine 171 mutated to Alanine

   Promoter

   CMV

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3.1-FLAG-Dicer-delHelicC

   Plasmid

   #51366

   Depositor

   Narry Kim

   Article

   Lee et al EMBO J. 2006 Feb 8;25(3):522-32. Epub 20

   Insert

   Dicer (DICER1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   From N terminus to HelicC domain deletion

   Promoter

   CMV

   Available Since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA 3.1 AURKA Lys162Met

    Plasmid

    #157751

    Purpose

    Expression of kinase-dead AURKA K62M in mammalian cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    AURKA K162M is a kinase-dead version of AURKA

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5/FRT/TO-GAlpha11-RLuc8

    Plasmid

    #140983

    Purpose

    Encodes a G alpha subunit (GNA11) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlpha11-RLuc8 (GNA11 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-FLAG-HA-CAD S1859A

    Plasmid

    #46239

    Purpose

    FLAG-HA-CAD with S1859 phosphorylation site mutated to alanine

    Depositor

    Brendan Manning

    Article

    Ben-Sahra et al Science. 2013 Mar 15;339(6125):1323-8. d

    Insert

    carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Serine 1859 changed to Alanine (S1859A)

    Promoter

    cmv

    Available Since

    Nov. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3.1-N-Myc_535-888 USP7

    Plasmid

    #131248

    Purpose

    Mammalian expression of the USP7 UBL1-3 domains with an N-terminal Myc tag.

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    A mutant form of our pcDNA3.1-N-Myc_535-1102 USP7…

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-N-Myc_884-1102 USP7

    Plasmid

    #131249

    Purpose

    Mammalian expression of the USP7 UBL4-5 domains with an N-terminal Myc tag

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Encodes USP7 UBL domains 4-5 (USP7 amino acids 88…

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. AIRAP 9: PSMD2.GST.V2.pcDNA3

    Plasmid

    #21799

    Depositor

    David Ron

    Article

    Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

    Insert

    PSMD2 (PSMD2 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1(+) EGFP-Rac1(P29S)

    Plasmid

    #224280

    Purpose

    Cell transfection and expression of Rac1 (P29S)

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    P29S

    Promoter

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA3-AUP1-FLAG-DATDCUE

    Plasmid

    #185340

    Purpose

    Assessing domain requirement sof AUP1

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    AUP1 (AUP1 Human)
    

    Use

    Tags

    FLAG, 6xHis

    Expression

    Mammalian

    Mutation

    deletion of aa 90-337 corresponding to the acyl t…

    Promoter

    Available Since

    May 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5-FRT-TO-N-Flag-8His

    Plasmid

    #157677

    Purpose

    N-terminal tagging vector

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Flag-8His
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 4, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pcDNA/Myc DeltaN TCF4

    Plasmid

    #16513

    Depositor

    Bert Vogelstein

    Article

    Korinek et al Science. 1997 Mar 21. 275(5307):1784-7.

    Insert

    TCF4 (TCF7L2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Delta N: lacks aa 1-32

    Promoter

    Available Since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3.1/NDUFS7/myc-His

    Plasmid

    #127488

    Purpose

    Expresses myc-tagged NDUFS7 in mammalian cells

    Depositor

    Dieter Wolf, Shashi Jain

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS7 (NDUFS7 Human)
    

    Use

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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