We narrowed to 13,037 results for: Mpl

1. pBx1-VHH template2-3XMyc-Spacer

   Plasmid

   #176209

   Purpose

   PCR template for generating CeVICA VHH/nanobody library

   Depositor

   Aviv Regev

   Article

   Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

   Insert

   VHH template2-3XMyc-Spacer
   

   Use

   Tags

   Myc tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. human SLC12A5-V5-HA-2A-Luciferase repair template

   Plasmid

   #158576

   Purpose

   For targeted insertion of V5-HA-2A-Luciferase reporter before the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.

   Depositor

   Rudolf Jaenisch

   Article

   Tang et al Sci Transl Med. 2019 Jul 31;11(503). pii

   Insert

   human SLC12A5 5' homology arm-V5-HA-2A-Luciferase-3' homology arm (SLC12A5 Human)
   

   Use

   Tags

   V5 and HA on the endogenous KCC2 protein product

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. lentiCRISPR - Amplicon, BCR/ABL sgRNA 1

   Plasmid

   #70659

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backbone

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   sgRNA against BCR/ABLamplicon found in CML-derived K562 cell line
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCR4-TOPO_L31-FLAG-TEV-AviTag_repair_template

   Plasmid

   #235608

   Purpose

   Repair template for inserting FLAG-TEV protease cleavage site-AviTag into the C terminus of mouse Rpl31 via CRISPR

   Depositor

   Maria Barna

   Article

   Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

   Insert

   Rpl31 C-terminus with FLAG-TEV-AviTag and flanking homology arms
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPD027 Reporter Template v1 (Near Spaced)

   Plasmid

   #138751

   Purpose

   Template for COMET promoters, expresses EYFP reporter upon promoter activation.

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pPD032 Reporter Template v1 (Mid Spaced)

   Plasmid

   #138753

   Purpose

   Template for COMET promoters, expresses EYFP reporter upon promoter activation.

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPD033 Reporter Template v1 (Far Spaced)

   Plasmid

   #138754

   Purpose

   Template for COMET promoters, expresses EYFP reporter upon promoter activation.

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s)

   Plasmid

   #47868

   Purpose

   This plasmid contains expression cassette for NmCas9 with N and C NLS and an HA tag, a cassette for expression of tracrRNA, a cassette for cloning crRNA under the control of U6 promoter.

   Depositor

   Erik Sontheimer, James Thomson

   Article

   Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

   Inserts

   NmCas9
   U6pr-tracrRNA
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and U6

   Available since

   Sept. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mNeonGreen-P2A-3xFLAG-hEEA1 HDR template

   Plasmid

   #214989

   Purpose

   Endogenously tag human EEA1 with an N-terminal 3xFLAG in addition to cytosolic mNG for cell sorting.

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   EEA1 (EEA1 Human)
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SOX9-T2A-H2B-EGFP repair template

    Plasmid

    #167972

    Purpose

    SOX9 reporter repair template

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    SRY-Box Transcription Factor 9 (SOX9 Human)
    

    Use

    CRISPR; Donor template

    Tags

    H2B-EGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge