We narrowed to 4,011 results for: plasmid lenti crispr

1. sgLenti-orthogonal

   Plasmid

   #105997

   Purpose

   Dual Streptococcus pyogenes and Staphylococcus aureus sgRNA expression vector

   Depositor

   Mike McManus

   Article

   Boettcher et al Nat Biotechnol. 2018 Jan 15. pii: nbt.40

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral; S.pyogenes sgrna expressio…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. sgDonor_lentiGuide

   Plasmid

   #184168

   Purpose

   LentiGuide-puro vector expressing the sgRNA necessary for linearization of the donor cassette plasmid.

   Depositor

   Ophir Shalem

   Article

   Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

   Insert

   sgDonor
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-TRS-Leader-SARS-CoV-2-EGFP

   Plasmid

   #171585

   Purpose

   Expression of 75 nt of the SARS-CoV-2 Leader sequence at 5' of destabilized eGFP reporter

   Depositor

   Neville Sanjana

   Article

   Mendez-Mancilla et al Cell Chem Biol. 2022 Feb 17;29(2):321-32

   Insert

   TRS-Leader-SARS-CoV-2-d2eGFP
   

   Use

   Lentiviral

   Tags

   2PEST AND NLS

   Expression

   Mutation

   Promoter

   Available since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. LentiGuide-BC-start

   Plasmid

   #127168

   Purpose

   Entry plasmid for two-step guide-barcode cloning

   Depositor

   Paul Blainey

   Article

   Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   N/A

   Available since

   June 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-AncBE4max

   Plasmid

   #174164

   Purpose

   This is an AncBE4max (a cytosine base editor) expressing plasmid built on the 3rd generation system of lentiviral vector. It can be used for stable expression of AncBE4max in cells.

   Depositor

   Wensheng Wei

   Article

   Xu et al Nat Biotechnol. 2021 Jun 21. pii: 10.103

   Insert

   AncBE4max
   

   Use

   Lentiviral

   Tags

   P2A-mCherry

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Direct-seq_lentiGuide-Puro-tRNA-Loop2-8A8G

   Plasmid

   #157986

   Purpose

   Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

   Depositor

   Lijia Ma

   Article

   Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

   Insert

   S. pyogenes sgRNA cassette (tRNA-Loop2-8A8G)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   May 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Direct-seq_lentiGuide-Puro-tRNA-Tail-8A8G

   Plasmid

   #157984

   Purpose

   Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

   Depositor

   Lijia Ma

   Article

   Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

   Insert

   S. pyogenes sgRNA cassette (tRNA-Tail-8A8G)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   March 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Direct-seq_lentiGuide-Puro-Tetra Loop-8A8G

   Plasmid

   #157982

   Purpose

   Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

   Depositor

   Lijia Ma

   Article

   Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

   Insert

   S. pyogenes sgRNA cassette (Tetra-8A8G)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   May 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. lenti GFAABC1D-dCas9-KRAB-MeCP2

   Plasmid

   #194701

   Purpose

   Expresses dCas9-KRAB-MeCP2 in Astrocytes

   Depositor

   Timothy Jarome

   Article

   Farrell et al Learn Mem. 2023 Mar 15;30(3):70-73. doi:

   Insert

   GFAABC1D promoter
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   None

   Promoter

   GFAABC1D

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-mCherry-Cre-blast

    Plasmid

    #179390

    Purpose

    Lentiviral expression of Cre recombinase and mCherry reporter from CMV promoter (separated by P2A peptide), and blasticidin resistance gene for selection in mammalian cells.

    Depositor

    Moshe Szyf

    Article

    Sapozhnikov et al Nat Commun. 2021 Sep 29;12(1):5711. doi:

    Insert

    mCherry (P2A) Cre recombinase
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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