We narrowed to 1,621 results for: sp1

1. pLIB_GST-nsp13 (SARS-CoV-2)

   Plasmid

   #169190

   Purpose

   Baculoviral transfer vector to express GST-nsp13 (SARS-CoV-2) in insect cells

   Depositor

   John Diffley

   Article

   Zeng et al Biochem J. 2021 Jul 16;478(13):2405-2423

   Insert

   GST-nsp13 (ORF1ab SARS-CoV-2, Synthetic)
   

   Use

   Tags

   GST

   Expression

   Insect

   Mutation

   Codon optimised for insect cells (Spodoptera frug…

   Promoter

   Polyhedrin

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-Xl-VSP1 R152Q GFP

   Plasmid

   #51884

   Purpose

   expression of Xl-VSP1 mutant fused to GFP in Xenopus oocytes or mammalian cells

   Depositor

   Laurinda Jaffe

   Article

   Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

   Insert

   VSP1 R152Q (tpte2 Frog)
   

   Use

   Xenopus oocyte expression

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   R152Q: near the extracellular side of the fourth …

   Promoter

   CMV

   Available since

   March 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. 7093 pNOTAG Hsp104 T317A

   Plasmid

   #1242

   Depositor

   Susan Lindquist

   Article

   Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

   Insert

   Hsp104 (T317A) (HSP104 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   T317A

   Promoter

   Available since

   Jan. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

4. pET22-Strep-TEV-Nsp10

   Plasmid

   #154958

   Purpose

   Expresses Nsp10 from SARS-CoV-2 with a N-terminal TEV-protease cleavable Strep tag in E. coli

   Depositor

   Justin Kollman

   Insert

   Strep-TEV-Nsp10 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Strep-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   July 21, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. p28BIOHTEV-LIC: nsp16_SARS-CoV-2

   Plasmid

   #167846

   Purpose

   Bacterial expression of the SARS-CoV-2 protein nsp16

   Depositor

   Cheryl Arrowsmith

   Article

   Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

   Insert

   nsp16
   

   Use

   Tags

   6xHis and AviTag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 16, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pPROEX Hsp104 (773-908)

   Plasmid

   #1316

   Depositor

   Susan Lindquist

   Article

   Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

   Insert

   Hsp104 (773-908) (HSP104 Budding Yeast)
   

   Use

   Tags

   6X His + TEV cleavage

   Expression

   Bacterial

   Mutation

   deletion of aa1-773

   Promoter

   Available since

   Jan. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPROEX Hsp104 (361-548)

   Plasmid

   #1317

   Depositor

   Susan Lindquist

   Article

   Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

   Insert

   Hsp104 (361-548) (HSP104 Budding Yeast)
   

   Use

   Tags

   6X His + TEV cleavage

   Expression

   Bacterial

   Mutation

   aa361-548 of Hsp104

   Promoter

   Available since

   Jan. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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8. 7090 pNOTAG Hsp104 N728A

   Plasmid

   #1244

   Depositor

   Susan Lindquist

   Article

   Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

   Insert

   Hsp104 (N728A) (HSP104 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N728A

   Promoter

   Available since

   Jan. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

9. 7092 pNOTAG Hsp104 R826M

   Plasmid

   #1243

   Depositor

   Susan Lindquist

   Article

   Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

   Insert

   Hsp104 (R826M) (HSP104 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   R826M

   Promoter

   Available since

   Jan. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

10. tet-pLKO shDUSP19 puro

    Plasmid

    #87796

    Purpose

    Expresses an inducible short hairpin targeting human DUSP19 sequence

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    shDUSP19 (DUSP19 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE tight

    Available since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHis-TEV-Nsp15-E266Q

    Plasmid

    #218803

    Purpose

    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-E266Q (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag

    Depositor

    Debra Hansen

    Article

    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:

    Insert

    Nsp15-E266Q (N Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    TEV protease cleavable His6-tag

    Expression

    Bacterial

    Mutation

    E266Q (G to C)

    Promoter

    Available since

    May 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHis-TEV-Nsp15-V66L

    Plasmid

    #219524

    Purpose

    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-V66L (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag

    Depositor

    Debra Hansen

    Article

    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:

    Insert

    Nsp15-V66L (N Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    TEV protease cleavable His6-tag

    Expression

    Bacterial

    Mutation

    V66L (G to C)

    Promoter

    Available since

    May 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHis-TEV-Nsp15-A81V

    Plasmid

    #219525

    Purpose

    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-A81V (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag

    Depositor

    Debra Hansen

    Article

    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:

    Insert

    Nsp15-A81V (N Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    TEV protease cleavable His6-tag

    Expression

    Bacterial

    Mutation

    A81V (C to T)

    Promoter

    Available since

    May 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHis-TEV-Nsp15-D183N

    Plasmid

    #219526

    Purpose

    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-D183N (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag

    Depositor

    Debra Hansen

    Article

    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:

    Insert

    Nsp15-D183N (N Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    TEV protease cleavable His6-tag

    Expression

    Bacterial

    Mutation

    D183N (G to A)

    Promoter

    Available since

    May 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHis-TEV-Nsp15-H234A

    Plasmid

    #219529

    Purpose

    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-H234A (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag

    Depositor

    Debra Hansen

    Article

    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:

    Insert

    Nsp15-H234A (N Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    TEV protease cleavable His6-tag

    Expression

    Bacterial

    Mutation

    H234A (CAC to GCG)

    Promoter

    Available since

    May 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMSIP267: pHelper(CyCAST)_PSP1_ΔTniQ

    Plasmid

    #200842

    Purpose

    Inducible expression of CyCAST proteins (except TniQ) with crRNA targeting CyPSP1.

    Depositor

    Feng Zhang

    Article

    Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

    Insert

    CyCAST
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMSIP269: pHelper(CyCAST)_PSP1_ΔTnsD

    Plasmid

    #200844

    Purpose

    Inducible expression of CyCAST proteins (except TnsD) with crRNA targeting CyPSP1.

    Depositor

    Feng Zhang

    Article

    Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

    Insert

    CyCAST
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMSIP116: pHelper(CyCAST)_PSP1

    Plasmid

    #200840

    Purpose

    Inducible expression of CyCAST proteins with crRNA targeting CyPSP1.

    Depositor

    Feng Zhang

    Article

    Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

    Insert

    CyCAST
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pT7-35aaNSP1SA11-Green Lantern(wt)

    Plasmid

    #220821

    Purpose

    Express the hybrid protein combining 35 amino acids from Rotavirus SA11 NSP1 protein with the Green Lantern wt protein carrying an ER-localization signal at the N- and C-termini

    Depositor

    Alex Borodavka

    Insert

    Rotavirus SA11 35aaNSP1-Green Latern(wt)
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT7-35aaNSP1SA11-Green Lantern(io)

    Plasmid

    #220822

    Purpose

    Express the hybrid protein combining 35 amino acids from Rotavirus SA11 NSP1 protein with the recoded Green Lantern protein carrying an ER-localization signal at the N- and C-termini

    Depositor

    Alex Borodavka

    Insert

    Rotavirus SA11 35aaNSP1-Green Latern(io)
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge