2,189 results
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Plasmid#109737PurposeMultisite gateway vector for 3' tagging with dominant negative rab5cDepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only
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pT7-gfap-sgRNA
Plasmid#65566Purposein vitro trancription of sgRNA targeting the zebrafish gfap locusDepositorAvailable SinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pXTm-zebrafish Tspan4a
Plasmid#210327PurposeExpresses Tetraspanin-4a (TSPAN4a) as migrasome markerDepositorInsertTetraspanin 4a (tspan4a Zebrafish)
UseFish expressionAvailable SinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMT-MCS-cytoBirA-2A-mCherry_Ras
Plasmid#80058PurposeMCS for cloning promoter to drive HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
Tags3x HAExpressionBacterialAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-ubb:MCP-p65-HSF1-2a-Cerulean-Ds
Plasmid#119074PurposeZebrafish ubiquitin promoter driving MCP-p65-HSF1 with Cerulean reporterDepositorInsertubb:MCP-p65-HSF1-2a-Cerulean
ExpressionBacterialPromoterZebrafish ubiquitin promoterAvailable SinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-ubb:Com-KRAB-2a-Cerulean-Ds
Plasmid#119075PurposeZebrafish ubiquitin promoter driving Com-KRAB with Cerulean reporterDepositorInsertubb:Com-KRAB-2a-Cerulean
ExpressionBacterialPromoterZebrafish ubiquitin promoterAvailable SinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-DrU6a:sgRNA-com-Ds
Plasmid#119077PurposeTo clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x com stem loopsDepositorInsertU6a:2xCom-sgRNA
ExpressionBacterialPromoterZebrafish U6a promoterAvailable SinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-DrU6a:sgRNA-MS2-Ds
Plasmid#119076PurposeTo clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x MS2 stem loopsDepositorInsertU6a:2xMS2-sgRNA
ExpressionBacterialPromoterZebrafish U6a promoterAvailable SinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
GBH-2aG4-gcryBFP.3
Plasmid#188015PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted 2aGal4VP16 protein trap; secondary marker gamma crystallin BFP
UseTargetable insertional mutagen and reporter systemPromoterEndogenuous Promoter; gamma crystallin promotorAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-R-gcryGFP.3
Plasmid#188000PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted mRFP protein trap; secondary marker gamma crystallin GFP
UseTargetable insertional mutagen and reporter systemPromoterEndogenuous Promoter; gamma crystallin promotorAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
Danio rerio cryabb
Plasmid#47665PurposeRecombinant protein productionDepositorAvailable SinceSept. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
Control-cx43.4-48H-pPRISM
Plasmid#117801PurposeControl to test technique on a target that is known to workDepositorInsertTagRFP
UseSynthetic BiologyAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEM-cytoBirA-2A-mCherry-SV40pA-FKF
Plasmid#79887PurposeDonor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertHA-tagged BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
ExpressionBacterialAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
UAS::PTPRFa.1-DN-GFP
Plasmid#37110DepositorInsertUAS::PTPRFa.1-DN-GFP (ptprf Zebrafish)
UseTol2 gateway expression vectorTagsEGFPMutationdeleted amino acids 1326-1894PromoterUAS-E1bAvailable SinceJuly 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Fuse-gcry-eGFP
Plasmid#117785PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertmRFP
UseSynthetic BiologyAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-TagRFP-cmlc2-mRFP
Plasmid#117769PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertTagRFP
UseSynthetic BiologyAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT2KXIGdeltaIn-MCS-birA-EGFP
Plasmid#58377Purposetol2 transgenesis for biotin based TRAP - BirA ligaseDepositorInsertbirA EGFP
TagsEGFPAvailable SinceAug. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pMT-myl7-cytoBirA-2A-mCherry_Ras
Plasmid#80060PurposeMyl7 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialPromoterMyl7 promoterAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only