2,188 results
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pMT-MCS-cytoBirA-2A-mCherry_Ras
Plasmid#80058PurposeMCS for cloning promoter to drive HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
Tags3x HAExpressionBacterialAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab8b in pCSDEST2
Plasmid#140881PurposeExpression of zebrafish GFP-tagged Rab8b in mammalian cells.DepositorAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
Control-cx43.4-48H-pPRISM
Plasmid#117801PurposeControl to test technique on a target that is known to workDepositorInsertTagRFP
UseSynthetic BiologyAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
UAS::PTPRFa.1-DN-GFP
Plasmid#37110DepositorInsertUAS::PTPRFa.1-DN-GFP (ptprf Zebrafish)
UseTol2 gateway expression vectorTagsEGFPMutationdeleted amino acids 1326-1894PromoterUAS-E1bAvailable SinceJuly 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEM-cytoBirA-2A-mCherry-SV40pA-FKF
Plasmid#79887PurposeDonor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertHA-tagged BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
ExpressionBacterialAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Fuse-gcry-eGFP
Plasmid#117785PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertmRFP
UseSynthetic BiologyAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-TagRFP-cmlc2-mRFP
Plasmid#117769PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertTagRFP
UseSynthetic BiologyAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
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LR (bact: HyPer)
Plasmid#162069PurposeUsed for the diffuse visualization of H2O2 production in cells.DepositorAvailable SinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMT-myl7-cytoBirA-2A-mCherry_Ras
Plasmid#80060PurposeMyl7 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialPromoterMyl7 promoterAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pPRISM-TagRFP-cmlc2-TagBFP
Plasmid#117768PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertTagRFP
UseSynthetic BiologyAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS-I-Sce1-slc6a3[11kb]:egfp
Plasmid#53240Purposeallow visualization of pretectal dopaminergic neurons in vivo from day 3 post-fertilization and allow expression of transgenes in these neurons through adulthoodDepositorInsertDAT-GFP
UseCloningTagsGFPAvailable SinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT2KXIGdeltaIn-MCS-birA-EGFP
Plasmid#58377Purposetol2 transgenesis for biotin based TRAP - BirA ligaseDepositorInsertbirA EGFP
TagsEGFPAvailable SinceAug. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
UAS::PTPRFb-DN-GFP
Plasmid#37111DepositorInsertUAS::PTPRFb-DN-GFP (ptprfb Zebrafish)
UseTol2 gateway expression vectorTagsEGFPMutationDeleted aa1386-1909 corresponding to phosphatase …Promoter10xUASAvailable SinceJuly 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-olig2
Plasmid#192743PurposeGateway entry vector encoding zebrafish olig2DepositorInsertolig2 (olig2 Zebrafish)
UseGateway entry vectorMutationS61A (rs501471300); G235S (rs505716173)PromoterNoneAvailable SinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-SERCA1
Plasmid#109498PurposeMuscle specific zebrafish expression of mKate2 fused to sarcoplasmic/endoplasmic calcium ATPase 1DepositorInsertSERCA1 (atp2a1 Zebrafish)
UseZebrafish plasmidsTagsmKate2MutationPlease see Depositor CommentsAvailable SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;lmo2P1Egfp
Plasmid#90162Purposecontains lmo2 specific gene promoter driving expression of eGFPDepositorAvailable SinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only
