2,172 results

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pGEM-and1 in situ probe

Plasmid

#52102

Purpose

actinodin 1 and1 antisense in situ probe linearize w/ ApaI SP6 pol

Depositor

Didier Stainier

Article

Nolte et al Proteomics. 2015 Feb;15(4):739-51. doi:

Insert

actinodin 1 in situ probe (and1 Zebrafish)

Expression

Bacterial

Available Since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

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pRM27

Plasmid

#163695

Purpose

ubb promoter driven expression of lck:mNeonGreen

Depositor

David Matus

Article

Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1

Insert

lck:mNeonGreen (lck Zebrafish)

Use

Zebrafish expression

Tags

mNeonGreen

Promoter

ubb

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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TALEN1260

Plasmid

#32282

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting gria3a (gria3a Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only

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TALEN1295

Plasmid

#32283

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting gria3a (gria3a Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only

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ZP36 Pax2

Plasmid

#16906

Depositor

Randall Moon

Insert

pax2 (pax2a Zebrafish)

Expression

Bacterial

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pDONR221-olig2

Plasmid

#192743

Purpose

Gateway entry vector encoding zebrafish olig2

Depositor

Summer Thyme

Insert

olig2 (olig2 Zebrafish)

Use

Gateway entry vector

Mutation

S61A (rs501471300); G235S (rs505716173)

Promoter

None

Available Since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;lmo2P1Egfp

Plasmid

#90162

Purpose

contains lmo2 specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

lmo2P1Egfp (lmo2 Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pBS-I-Sce1-slc6a3[11kb]:egfp

Plasmid

#53240

Purpose

allow visualization of pretectal dopaminergic neurons in vivo from day 3 post-fertilization and allow expression of transgenes in these neurons through adulthood

Depositor

Edward Burton

Article

Bai et al Neuroscience. 2009 Dec 15;164(3):1138-51

Insert

DAT-GFP

Use

Cloning

Tags

GFP

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-SERCA1

Plasmid

#109498

Purpose

Muscle specific zebrafish expression of mKate2 fused to sarcoplasmic/endoplasmic calcium ATPase 1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

SERCA1 (atp2a1 Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Mutation

Please see Depositor Comments

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pMT-myl7-cytoBirA-2A-mCherry_Ras

Plasmid

#80060

Purpose

Myl7 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Article

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein

Use

Zebrafish biotagging

Tags

3x HA

Expression

Bacterial

Promoter

Myl7 promoter

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pT2KXIGdeltaIn-MCS-birA-EGFP

Plasmid

#58377

Purpose

tol2 transgenesis for biotin based TRAP - BirA ligase

Depositor

Didier Stainier

Article

Housley et al Development. 2014 Oct;141(20):3988-93. d

Insert

birA EGFP

Tags

EGFP

Available Since

Aug. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pDest-1.7kbfabp6-eGFP-pA-CrymCherry

Plasmid

#159088

Purpose

LR construct using backbone with Cry:mCherry insert to identify fish with transgene, and with p5E-1.7kbfabp6, pME-eGFP, and p3E-pA inserts

Depositor

John Rawls

Article

Wen et al Sci Adv. 2021 Jul 23;7(30). pii: 7/30/ea

Inserts

1.7kb fabp6 promoter (fabp6 Zebrafish)
eGFP
polyA

Use

Fluorescent reporter

Promoter

1.7kb fabp6

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-Opto-Alk8

Plasmid

#207615

Purpose

Zebrafish Alk8 BMP receptor kinase domain fused to VfLOV

Depositor

Patrick Müller

Article

Rogers et al Elife. 2020 Nov 11;9:e58641. doi: 10.755

Insert

Alk8 (acvr1l) kinase domain + VfLOV domain (acvr1l Zebrafish, Vaucheria frigida)

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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SI16-Kit a

Plasmid

#58948

Purpose

expresses recombinant mouse anti-Kit a receptor (zebrafish) IgG1 antibody in mammalian cells

Depositor

Gavin Wright

Article

Crosnier et al BMC Biol. 2010 Jun 4;8:76. doi: 10.1186/

Insert

Kit receptor a mouse monoclonal antibody (kita Zebrafish)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
pCS2 you

Plasmid

#11084

Depositor

William Talbot

Article

Woods et al PLoS Biol. 2005 Mar . 3(3):e66.

Insert

you (scube2 Zebrafish)

Use

Zebrafish

Available Since

Dec. 8, 2005

Availability

Academic Institutions and Nonprofits only
pGEM-T Easy-Gbait-hs-QF2

Plasmid

#122563

Purpose

Integration of QF2 sequence using the CRISPR/Cas9 system.

Depositor

Marnie Halpern

Article

Choi et al Elife. 2021 Dec 8;10:e72345. doi: 10.755

Insert

Gbait-hsp70-QF2-SV40pA

Use

Cloning

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pPRISM-Stop-cmlc2-mRFP

Plasmid

#117775

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

3x3 Stop sequence

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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ZE15 pCS2P+ wnt8 ORF2

Plasmid

#17049

Depositor

Randall Moon

Insert

wnt8 (wnt8a Zebrafish)

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pT2-cryR;tmem88aCE1-P1Egfp

Plasmid

#90148

Purpose

contains tmem88a enhancer sequence, and tmem88a specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

tmem88aE1P1Egfp (tmem88a Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pTol2-DRv6

Plasmid

#78628

Purpose

GESTALT V6 barcode for zebrafish

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

GESTALT lineage tracing target V6

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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