We narrowed to 4,220 results for: PRS
-
Plasmid#221124PurposeFAP-tagged marker for the trans-Golgi (FAP optimized for yeast expression)DepositorInsertSEC7-FAPoptim
ExpressionYeastPromoterTEF1Available SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-TEF1pr-VPH1-FAPoptim
Plasmid#221125PurposeFAP-tagged marker for the vacuolar membrane (FAP optimized for yeast expression)DepositorInsertVPH1-FAPoptim
ExpressionYeastPromoterTEF1Available SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
CUP1-∆ssCPY*-mNeonGreen in pRS315
Plasmid#221160PurposeFluorescent reporter for cytoplasmic aggregates under LEU2 selection markerDepositorInsertCarboxypeptidase Y (PRC1 Budding Yeast)
TagsmNeonGreenExpressionYeastMutationDeletion signal peptide, G255A mutationAvailable SinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS415 GPDpro-RNQ1-CFP
Plasmid#224887PurposeLow copy plasmid for constitutive yeast expression of RNQ1 tagged with CFP using copper. This is construct can be used as an IPOD marker (Kaganovich et al., 2008).DepositorAvailable SinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF-DUET-C17orf53_1-87-Strep
Plasmid#211433Purposebacterial expression of the N-terminal amino acids 1-87 of human C17orf53 C-terminally fused to a Strep-TagDepositorAvailable SinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF-Duet-C17orf53-6His
Plasmid#211417Purposebacterial expression of human C17orf53 C-terminally fused to 6xHis-TagDepositorAvailable SinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF-Duet-6His-C17orf53
Plasmid#211416Purposebacterial expression of human C17orf53 N-terminally fused to a 6xHis-TagDepositorAvailable SinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF- RPA4 (MSW#716)
Plasmid#208077PurposeExpression of human Replication Protein A 4 in E.coliDepositorInsertRPA4 (RPA4 Human)
ExpressionBacterialAvailable SinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSET-WHaloCaMP-eNOSpep-EGFP
Plasmid#205306PurposeExpression of WHaloCaMP-eNOSpep-EGFP fusion in E. coliDepositorInsertWHaloCaMP-eNOSpep-EGFP
ExpressionBacterialAvailable SinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-HsDCP1a_539-582 (MCSII)_G
Plasmid#146407PurposeBacterial Expression of HsDCP1a_539-582DepositorInsertHsDCP1a_539-582 (DCP1A Human)
ExpressionBacterialMutationone silent mutation T1716G compared to the sequen…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetP-HsPatL1_517-767del664-673_J
Plasmid#146671PurposeBacterial Expression of HsPatL1_517-767-del664-673DepositorInsertHsPatL1_517-767-del664-673 (PATL1 Human)
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetP-HsPatL1_517-770-Mut1_J
Plasmid#146672PurposeBacterial Expression of HsPatL1_517-770-Mut1DepositorInsertHsPatL1_517-770-Mut1 (PATL1 Human)
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetP-HsPatL1_517-770-Mut2_J
Plasmid#146673PurposeBacterial Expression of HsPatL1_517-770-Mut2DepositorInsertHsPatL1_517-770-Mut2 (PATL1 Human)
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetP-HsPatL1_517-770-Mut3_J
Plasmid#146674PurposeBacterial Expression of HsPatL1_517-770-Mut3DepositorInsertHsPatL1_517-770-Mut3 (PATL1 Human)
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetP-HsPatL1_517-770-R591AR595AK625AK626A_J
Plasmid#146675PurposeBacterial Expression of HsPatL1_517-770-R591AR595AK625AK626ADepositorInsertHsPatL1_517-770-R591AR595AK625AK626A (PATL1 Human)
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-mNeongreen-Uricase
Plasmid#182417PurposeBacterial expression of Vesicle Nucleating peptide-mNeongreen-uricase fusionDepositorInsertVNp-mNeongreen-uricase
TagsVesicle Nucleating peptide (VNp) & mNeongreenExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-LZ_CydAB-mNeongreen
Plasmid#182393PurposeBacterial expression of Vesicle Nucleating peptide-Leucine Zipper and cydAB--mNeongreen fusionsDepositorInsertsVNp-LZ
CydAB-mNeongreen
TagsVesicle Nucleating peptide (VNp)ExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-DmXRN1_1-573 (MCSI)_E
Plasmid#146176PurposeBacterial Expression of DmXRN1_1-573DepositorInsertDmXRN1_1-573 (pcm Fly)
ExpressionBacterialAvailable SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-HsDCP1a_1-135 (MCSII)_E
Plasmid#146177PurposeBacterial Expression of HsDCP1a_1-135DepositorInsertHsDCP1a_1-135 (DCP1A Human)
ExpressionBacterialAvailable SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-HsEDC3_192-228 (MCSII)_E
Plasmid#146178PurposeBacterial Expression of HsEDC3_192-228DepositorInsertHsEDC3_192-228 (EDC3 Human)
ExpressionBacterialAvailable SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS306-PTDH3-Dimer-1SUMO
Plasmid#190249PurposeExpression dimer-1SUMO component in yeast cellsDepositorInsertdimer-SUMO
ExpressionYeastAvailable SinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS306-PTDH3-SUMO7-6SIM
Plasmid#190250PurposeExpression SUMO7-SIM6 in yeast cellsDepositorInsert7SUMO-6SIM
ExpressionYeastAvailable SinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-DmDCP1_128-372 (MCSI)_E
Plasmid#146174PurposeBacterial Expression of DmDcp1_128-372DepositorAvailable SinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-DmDCP1_1-127 (MCSII)_E
Plasmid#146173PurposeBacterial Expression of DmDCP1_1-127DepositorInsertDmDCP1_1-127 (Dcp1 Fly)
ExpressionBacterialAvailable SinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-DmDCP1_328-366 (MCSII)_G
Plasmid#146402PurposeBacterial Expression of DmDCP1_328-366DepositorInsertDmDCP1_328-366 (Dcp1 Fly)
ExpressionBacterialAvailable SinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSF-1b-S7-S83C/Δ157-178
Plasmid#128592PurposeExpresses tagless S7-S83C, Δ157-178 mutant 30S ribosomal protein in E.coliDepositorInsertS7 E.coli ribosomal protein (rpsG E.coli)
ExpressionBacterialMutationS83C, Δ157-178PromoterT7Available SinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetMP-CeSmg7_1-395-G80E_S
Plasmid#147476PurposeBacterial Expression of CeSmg7_1-395-G80EDepositorInsertCeSmg7_1-395-G80E (smg-7 Nematode)
ExpressionBacterialAvailable SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-HsPatL1_1-111 (MCSII)_B
Plasmid#145968PurposeBacterial Expression of HsPatL1_1-111DepositorInsertHsPatL1_1-111 (PATL1 Human)
ExpressionBacterialAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-HsPatL1_1-173 (MCSII)_B
Plasmid#145969PurposeBacterial Expression of HsPatL1_1-173DepositorInsertHsPatL1_1-173 (PATL1 Human)
ExpressionBacterialAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-HsPatL1_1-398 (MCSII)_B
Plasmid#145970PurposeBacterial Expression of HsPatL1_1-398DepositorInsertHsPatL1_1-398 (PATL1 Human)
ExpressionBacterialAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-HsRCK_296-472(MCSI)_B
Plasmid#145971PurposeBacterial Expression of HsRck_296-472DepositorInsertHsRck_296-472 (DDX6 Human)
ExpressionBacterialAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-HsRCK-HsPatL1_1-59_B
Plasmid#145973PurposeBacterial Expression of HsPatL1_1-59DepositorInsertHsPatL1_1-59 (PATL1 Human)
ExpressionBacterialAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-HsRCK-HsPatL1_1-173_B
Plasmid#145975PurposeBacterial Expression of HsPatL1_1-173DepositorInsertHsPatL1_1-173 (PATL1 Human)
ExpressionBacterialAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-HsRCK-HsPatL1_1-398_B
Plasmid#145976PurposeBacterial Expression of HsPatL1_1-398DepositorInsertHsPatL1_1-398 (PATL1 Human)
ExpressionBacterialAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSET-QUEEN 7mu R126A
Plasmid#129376Purposemutant of QUEEN-7muDepositorInsertQUEEN 7mu R126A
ExpressionBacterialAvailable SinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSET-QUEEN 7mu R126I
Plasmid#129377Purposemutant of QUEEN-7muDepositorInsertQUEEN 7mu R126I
ExpressionBacterialAvailable SinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSET-QUEEN 7mu A81I
Plasmid#129352Purposemutant of QUEEN-7muDepositorInsertQUEEN 7mu A81I
ExpressionBacterialAvailable SinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSET-QUEEN 7mu D89A
Plasmid#129354Purposemutant of QUEEN-7muDepositorInsertQUEEN 7mu D89A
ExpressionBacterialAvailable SinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSET-QUEEN 7mu V90D
Plasmid#129355Purposemutant of QUEEN-7muDepositorInsertQUEEN 7mu V90D
ExpressionBacterialAvailable SinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSET-QUEEN 7mu A81E
Plasmid#129356Purposemutant of QUEEN-7muDepositorInsertQUEEN 7mu A81E
ExpressionBacterialAvailable SinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSET-QUEEN 7mu I88D
Plasmid#129357Purposemutant of QUEEN-7muDepositorInsertQUEEN 7mu I88D
ExpressionBacterialAvailable SinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1-His-TEV-OTC_d1_Ub
Plasmid#170729PurposeScaffold design for studying ubiquitin interacting proteins by cryo-EM.DepositorInsertE. coli ornithine transcarbamylase (OTC), fused to human ubiquitin
Tags6xHis-TEVExpressionBacterialMutationThe C-terminus of OTC was truncated by removing o…PromoterT7Available SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS41K-mCherry-mNeonGreen (pJJF003)
Plasmid#173459PurposeC-SWAT donor for Seamless tagging, contains mCherry-mNeonGreen tandem fluorescent protein timerDepositorInsertmCherry-mNeonGreen
ExpressionYeastPromoterno promoterAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-HsRCK-HsPatL1_1-111_B
Plasmid#145974PurposeBacterial Expression of HsPatL1_1-111DepositorInsertHsPatL1_1-111 (PATL1 Human)
ExpressionBacterialAvailable SinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS406 ATG1-ATG7-3'UTR
Plasmid#166847PurposeExpresses Atg1 with ATG7 3'UTR in yeasts.DepositorAvailable SinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS405 SEC66-VAC8-GFP
Plasmid#166840PurposeExpresses ER localized Vac8-GFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.DepositorTagsGFP and SEC66 transmembrane domainExpressionYeastMutationSEC66 transmembrane domainPromoterVAC8 promoterAvailable SinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS405 VAC8-G2A-GFP
Plasmid#166837PurposeExpresses a myristoylation mutant Vac8-GFP in yeasts, mutation is Gly2AlaDepositorAvailable SinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1-tglA(C51)HIB
Plasmid#134675PurposeExpresses His6-TglA(C51), TglH, TglI and TglB in E. ColiDepositorInsertsTglA(C51)
TglH
TglI
TglB
TagsHis6ExpressionBacterialMutationinsertion of cysteine 51PromoterT7Available SinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PTEF1-MCP-3P-ECM33_intron
Plasmid#127601Purposelow-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 3P-ECM33 intron inserted into URA3DepositorInsertECM33 intron with MS2 hairpins inserted near 3′ end of intron
ExpressionYeastAvailable SinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PTEF1-MCP-5M-ECM33_intron
Plasmid#127600Purposelow-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5M-ECM33 intron inserted into URA3DepositorInsertECM33 intron with MS2 hairpins replacing sequence near 5′ end of intron
ExpressionYeastAvailable SinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only