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pCHKU27.2-2.2

Plasmid

#110662

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCHSU75.2-2.2

Plasmid

#110691

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCHKU33.2-2.2

Plasmid

#110667

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5, S_kudeiaveseii_ADH2-ORF6

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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CSNK1G2 gRNA (BRDN0001162501)

Plasmid

#76988

Purpose

3rd generation lentiviral gRNA plasmid targeting human CSNK1G2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CSNK1G2 (CSNK1G2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CSNK1G2 gRNA (BRDN0001149101)

Plasmid

#76989

Purpose

3rd generation lentiviral gRNA plasmid targeting human CSNK1G2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CSNK1G2 (CSNK1G2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
CSNK1G2 gRNA (BRDN0001145026)

Plasmid

#76990

Purpose

3rd generation lentiviral gRNA plasmid targeting human CSNK1G2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CSNK1G2 (CSNK1G2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiCRISPR v2-sgHSPC300-1

Plasmid

#210141

Purpose

knock out HSPC300 in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Protein BRICK1 (BRK1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgHSPC300-2

Plasmid

#210142

Purpose

knock out HSPC300 in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Protein BRICK1 (BRK1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
KC1G1_HUMAN_D0

Plasmid

#79691

Purpose

This plasmid encodes the kinase domain of KC1G1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

Markus Seeliger, John Chodera, Nicholas Levinson

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KC1G1 (CSNK1G1 Human)

Use

Tags

His10-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
CSNK1G2 gRNA (BRDN0001162426)

Plasmid

#76991

Purpose

3rd generation lentiviral gRNA plasmid targeting human CSNK1G2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CSNK1G2 (CSNK1G2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-DDR1-V5/HIS

Plasmid

#201100

Purpose

expression of human DDR1 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

human DDR1 receptor tyrosine kinase, full length, wildtype (DDR1 Human)

Use

Tags

V5/His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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DCLK1 gRNA (BRDN0001146493)

Plasmid

#76748

Purpose

3rd generation lentiviral gRNA plasmid targeting human DCLK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DCLK1 (DCLK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
DCLK1 gRNA (BRDN0001147487)

Plasmid

#76747

Purpose

3rd generation lentiviral gRNA plasmid targeting human DCLK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DCLK1 (DCLK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
DCLK1 gRNA (BRDN0001145902)

Plasmid

#76746

Purpose

3rd generation lentiviral gRNA plasmid targeting human DCLK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DCLK1 (DCLK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
CL20_mEGFP_PCBD2_KRT13

Plasmid

#205892

Purpose

Express mEGFP-tagged fusion protein, PCBD2_KRT13 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

PCBD2_KRT13 (PCBD2, KRT13 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CSNK1G1 gRNA (BRDN0001148117)

Plasmid

#77438

Purpose

3rd generation lentiviral gRNA plasmid targeting human CSNK1G1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CSNK1G1 (CSNK1G1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
CSNK1G1 gRNA (BRDN0001147566)

Plasmid

#77439

Purpose

3rd generation lentiviral gRNA plasmid targeting human CSNK1G1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CSNK1G1 (CSNK1G1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
CSNK1G1 gRNA (BRDN0001146949)

Plasmid

#77440

Purpose

3rd generation lentiviral gRNA plasmid targeting human CSNK1G1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CSNK1G1 (CSNK1G1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
CSNK1G1 gRNA (BRDN0001146475)

Plasmid

#77441

Purpose

3rd generation lentiviral gRNA plasmid targeting human CSNK1G1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CSNK1G1 (CSNK1G1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
SF-SON IRES Puro

Plasmid

#214402

Purpose

Expresses human SOX10, OLIG2, NKX6.2 and PAC (puromycin N-acetyl-transferase)

Depositor

Tanja Kuhlmann

Article

Ehrlich et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

SOX10, Olig2, NKX6.2 (SOX10, OLIG2, NKX6-2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge