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pcDNA3.3 Gag-imCherry

Plasmid

#208882

Purpose

codes for Gag from HIV-1 with an internal mCherry fluorescent protein

Depositor

Raphael Gaudin

Article

Diana Brychka et al bioRxiv

Insert

HIV-1 Gag

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCry2PHR-mCh-LRP6c

Plasmid

#42960

Purpose

Expresses optogenetically controlled LRP6 (C-terminal domain)

Depositor

David Schaffer

Article

Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

Insert

Cry2PHR-mCherry-LRP6c

Use

Tags

Expression

Mammalian

Mutation

LPR6 cytoplasmic domain only

Promoter

CMV

Available since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

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pKO-II-miR-17~92

Plasmid

#19029

Depositor

Tyler Jacks

Article

Ventura et al Cell. 2008 Mar 7. 132(5):875-86.

Insert

microRNA 17-92 (Mir17 Mouse)

Use

Cre/Lox and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only

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Syntaxin4-myc-myc-His

Plasmid

#12377

Depositor

Thomas Weimbs

Article

Low et al Mol Biol Cell. 2006 Feb . 17(2):977-89.

Insert

Syntaxin4 (STX4 Human)

Use

Tags

2xMyc-His

Expression

Mammalian

Mutation

Cloned in modified PCDNA4 vector (double myc)

Promoter

CMV⁄TO

Available since

Sept. 28, 2006

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 sgIREB2_1

Plasmid

#169894

Purpose

Disrupt IREB2

Depositor

Richard Possemato

Article

Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

Insert

sgRNA targeting IREB2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-FAK W266A

Plasmid

#186149

Purpose

For expressing FAK in mammalian cells

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PTK2 (PTK2 Mouse)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

cmv

Available since

July 1, 2022

Availability

Academic Institutions and Nonprofits only

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pEYFP-C3-HA-Ubiquitin

Plasmid

#28236

Depositor

Michael Mancini

Article

Stenoien et al Nat Cell Biol. 2002 Oct . 4(10):806-10.

Insert

Ubiquitin (UBB Human)

Use

Tags

EYFP and HA

Expression

Mammalian

Mutation

Promoter

Available since

April 7, 2011

Availability

Academic Institutions and Nonprofits only

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pLV-SFFV-ZFYVE27-WPRE-UbC-mCherry

Plasmid

#214470

Purpose

Lentiviral vector plasmid expressing human zinc finger FYVE-type containing 27 (ZFYVE27) under the spleen focus-forming virus (SFFV) promoter and mCherry under the Ubiquitin C (UbC) promoter

Depositor

Bart Nieuwenhuis, Richard Eva

Inserts

ZFYVE27 (ZFYVE27 Human)
mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLEX-FKBP-Rab7a-Blasticidin

Plasmid

#120716

Purpose

Expresses late endosome-targeted recruiter protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

Rab7a

Use

Lentiviral

Tags

FKBP and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-p53 (NES-)

Plasmid

#12092

Depositor

Tyler Jacks

Article

Boyd et al Nat Cell Biol. 2000 Sep . 2(9):563-8.

Insert

p53 (NES-) (TP53 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

L348A L350A Localized to nucleus.

Promoter

Available since

Aug. 7, 2006

Availability

Academic Institutions and Nonprofits only

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pCry2PHR-mCh-Rac1

Plasmid

#42954

Purpose

Expresses optogenetically controlled Rac1

Depositor

David Schaffer

Article

Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

Insert

Cry2PHR-mCherry-Rac1 (RAC1 Human, Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

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pOD_009

Plasmid

#159520

Purpose

Rifampicin version of the scarless genome engineering pDEL plasmid (Tikh et al. 2016) compatible with Salmonella

Depositor

Elisabeth Raleigh

Article

Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. d

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 30, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV HDM2(C464A)

Plasmid

#12086

Depositor

Tyler Jacks

Article

Boyd et al Nat Cell Biol. 2000 Sep . 2(9):563-8.

Insert

HDM2 (MDM2 Human)

Use

Tags

Expression

Mammalian

Mutation

C464A: RING-finger-domain mutant

Promoter

Available since

June 8, 2006

Availability

Academic Institutions and Nonprofits only
pJBJZ_001

Plasmid

#155357

Purpose

Gentamycin derivative of lambda-red pKD3 plasmid from Datsenko and Wanner (2000)

Depositor

Elisabeth Raleigh

Article

Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. d

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 30, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pJBJZ_006

Plasmid

#155359

Purpose

Zeomycin derivative of lambda-red pKD3 plasmid from Datsenko and Wanner (2000)

Depositor

Elisabeth Raleigh

Article

Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. d

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 30, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pGem-PgpdA-Cre-TtrpC

Plasmid

#168784

Purpose

Helper vector for the transient expression of Cre recombinase in filamentous fungi. Not replicative in fungi.

Depositor

Yit Heng Chooi

Article

Roux et al ACS Synth Biol. 2022 Feb 16. doi: 10.102

Insert

Cre

Use

Cre/Lox and Synthetic Biology; Expression in fila…

Tags

Expression

Mutation

Promoter

PgpdA

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pWBC

Plasmid

#15239

Depositor

Ron Vale

Article

Niclas et al Neuron. 1994 May . 12(5):1059-72.

Insert

Human neuronal kinesin heavy chain (KIF5A Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 12, 2007

Availability

Academic Institutions and Nonprofits only
BbZIP-cALFA

Plasmid

#160498

Purpose

For the protein expression of BbZIP with ALFA tag at C-terminus in E. coli

Depositor

Motoyuki Hattori

Article

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Insert

BbZIP

Use

Tags

HRV3C protease cleavage site-ALFA tag-8histidine

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pHRW-CRY2olig-VHH(GFP)

Plasmid

#126023

Purpose

express RFP-tagged CRY2 PHR E490G fused to anti-GFP nanobody for optogenetics

Depositor

Eurico Morais-de-Sá

Article

Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

Insert

CRY2 PHRolig-VHH(GFP)

Use

Tags

RFP

Expression

Insect

Mutation

CRY2 E490G

Promoter

Hsp70

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pMetTU1-A_Sv5K_araC-PBAD_MCD1_MegaB-S9G-R31L-R85L_MegaRNFGLSKJTU_Bba-B0015

Plasmid

#202025

Purpose

Expresses Bacillus megaterium ARG cluster (GvpB S9G-R31L-R85L mutant) in E. coli

Depositor

Mikhail Shapiro

Article

Hurt et al bioRxiv 2024.03.30.587094

Insert

Bacillus megaterium ARG cluster (GvpB S9G-R31L-R85L mutant)

Use

Tags

Expression

Bacterial

Mutation

GvpB S9G-R31L-R85L

Promoter

Available since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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