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Lenti-guide-puro mBltp1 (KIAA1109) - 2

Plasmid

#198490

Purpose

lentiviral stable expression of mBltp1 (KIAA1109) gRNA 2

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mBltp1 (4932438A13Rik Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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Jam2-Fc-His

Plasmid

#72079

Purpose

Expresses the extracellular region of the JAM-2 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Jam2 (Jam2 Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 Neo PTEN sgRNA5

Plasmid

#233246

Purpose

Knock out of murine PTEN

Depositor

David Bryant

Article

Nikolatou et al EMBO J. 2023 Sep 18;42(18):e113987. doi:

Insert

PTEN (Pten Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 Neo PTEN sgRNA2

Plasmid

#233245

Purpose

Knock out of murine PTEN

Depositor

David Bryant

Article

Nikolatou et al EMBO J. 2023 Sep 18;42(18):e113987. doi:

Insert

PTEN (Pten Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFP-SET-EMD (N-SET-EMD)

Plasmid

#217765

Purpose

Fluorescent reporter for ceramide (putative, mammalian expression)

Depositor

Paula Nunes-Hasler

Article

Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

Insert

SET (SET Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

EMD domain (aa 70-226)

Promoter

CMV

Available since

June 12, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pSET-EMD-EGFP (C-SET-EMD)

Plasmid

#217764

Purpose

Fluorescent reporter for ceramide (putative, mammalian expression)

Depositor

Paula Nunes-Hasler

Article

Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

Insert

SET (SET Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

EMD domain (aa 70-226)

Promoter

CMV

Available since

June 12, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-KSR1-CA3 (N-KSR)

Plasmid

#217755

Purpose

Fluorescent reporter for glucosyl-ceramide (putative, mammalian expression)

Depositor

Paula Nunes-Hasler

Article

Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

Insert

Kinase suppressor of ras 1 CA3 domain (KSR1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

CA3 domain aa 317-400

Promoter

CMV

Available since

June 11, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pKSR1-CA3-GS-mRFP1 (C-KSR-GS)

Plasmid

#217758

Purpose

Fluorescent reporter for ceramide (mammalian expression)

Depositor

Paula Nunes-Hasler

Article

Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

Insert

Kinase suppressor of ras 1 CA3 domain (KSR1 Human)

Use

Tags

mRFP

Expression

Mammalian

Mutation

CA3 domain aa 317-400 with GGSSGGGGA linker

Promoter

CMV

Available since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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pKSR1-CA3-GS-EGFP (C-KSR-GS)

Plasmid

#217757

Purpose

Fluorescent reporter for ceramide (mammalian expression)

Depositor

Paula Nunes-Hasler

Article

Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

Insert

Kinase suppressor of ras 1 CA3 domain (KSR1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

CA3 domain aa 317-400 with GGSSGGGGA linker

Promoter

CMV

Available since

June 10, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pKSR1-CA3-EGFP (C-KSR)

Plasmid

#217753

Purpose

Fluorescent reporter for ceramide (mammalian expression)

Depositor

Paula Nunes-Hasler

Article

Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

Insert

Kinase suppressor of ras 1 CA3 domain (KSR1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

CA3 domain aa 317-400 plus vector-based linker LE…

Promoter

CMV

Available since

June 10, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
Anti-TrpM7 [N74/25R]

Plasmid

#177569

Purpose

Mammalian Expression Plasmid of anti-TrpM7 (Mouse). Derived from hybridoma N74/25.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-TrpM7 (Mus musculus) recombinant mouse monoclonal antibody (Trpm7 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pSbi-pur-EGFP-KSR1-CA3 (N-KSR)

Plasmid

#217769

Purpose

Fluorescent reporter for glucosyl-ceramide (putative, mammalian expression)

Depositor

Paula Nunes-Hasler

Article

Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

Insert

Kinase suppressor of ras 1 CA3 domain (KSR1 Human)

Use

Sleeping beauty transposase-compatible genome ins…

Tags

EGFP

Expression

Mammalian

Mutation

CA3 domain aa 317-400

Promoter

EF-1alpha promoter

Available since

June 10, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pKSR1-CA3-mRFP1 (C-KSR)

Plasmid

#217754

Purpose

Fluorescent reporter for ceramide (mammalian expression)

Depositor

Paula Nunes-Hasler

Article

Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

Insert

Kinase suppressor of ras 1 CA3 domain (KSR1 Human)

Use

Tags

mRFP

Expression

Mammalian

Mutation

CA3 domain aa 317-400 plus vector-based linker LE…

Promoter

CMV

Available since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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pSbi-pur-KSR1-CA3-EGFP (C-KSR)

Plasmid

#217768

Purpose

Fluorescent reporter for ceramide (for stable mammalian expression)

Depositor

Paula Nunes-Hasler

Article

Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

Insert

Kinase suppressor of ras 1 CA3 domain (KSR1 Human)

Use

Sleeping beauty transposase-compatible genome ins…

Tags

EGFP

Expression

Mammalian

Mutation

CA3 domain aa 317-400 plus vector-based linker LE…

Promoter

EF-1alpha promoter

Available since

June 10, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pETM11-SUMO3- sfGFP-KSR1-CA3 (N-KSR)

Plasmid

#217767

Purpose

Fluorescent reporter for glucosyl-ceramide (putative, mammalian expression)

Depositor

Paula Nunes-Hasler

Article

Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

Insert

Kinase suppressor of ras 1 CA3 domain (KSR1 Human)

Use

Protein purification (6xhis tag, tev site, sumo3 …

Tags

sfGFP

Expression

Bacterial

Mutation

CA3 domain aa 317-400

Promoter

T7 promoter

Available since

June 10, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pETM11-SUMO3-KSR1-CA3-sfGFP (C-KSR)

Plasmid

#217766

Purpose

Fluorescent reporter for ceramide (for bacterial expression and purification)

Depositor

Paula Nunes-Hasler

Article

Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

Insert

Kinase suppressor of ras 1 CA3 domain (KSR1 Human)

Use

Protein purification (6xhis tag, tev site, sumo3 …

Tags

sfGFP

Expression

Bacterial

Mutation

CA3 domain aa 317-400 plus vector-based linker LE…

Promoter

T7 promoter

Available since

June 10, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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Lenti-sgPten#2/Cre

Plasmid

#173646

Purpose

Expresses a Pten-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Pten (Pten Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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px330-Pten-2

Plasmid

#162552

Purpose

sgRNA targeting Pten

Depositor

Amaia Lujambio

Article

Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

Insert

sgRNA targeting Pten (Pten Mouse)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

U6

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-Arl13b [N295B/66R]

Plasmid

#140076

Purpose

Mammalian Expression Plasmid of anti-Arl13b (Mouse). Derived from hybridoma N295B/66.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Arl13b (Mouse) recombinant mouse monoclonal antibody (Arl13b Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgCrls1-1#

Plasmid

#234765

Purpose

knockout Crls1

Depositor

Judy Lieberman

Article

Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

Insert

Crls1 (Crls1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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