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We narrowed to 450 results for: crispr library

Showing: 421 - 440 of 450 results

  1. pLentiRNAGuide_004-hU6-RfxCas13d-DR1-BsmBI-EFS-EGFP-P2A-Puro-WPRE

    Plasmid
    #223175
    Purpose
    For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa
    Depositor
    Neville Sanjana
    Article
    Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.
    Insert
    hU6_Rfxcas13d-DR1_BsmBI
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    DR1 A>T
    Promoter
    Available Since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    223175-plasmid-map-sequence-id-457165

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  3. pLenti_mpx_AsCas12a(dual-gRNA)_h7SK(PacI-ClaI)_U6(I-SceI-NheI)_PGK-puro

    Plasmid
    #189635
    Purpose
    Lentiviral expression of double dual-AsCas12a gRNAs for generating combinatorial AsCas12a 3Cs libraries
    Depositor
    Manuel Kaulich
    Article
    Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.
    Insert
    h7SK arrayed Cas12a gRNA cassette, hU6 arrayed Cas12a gRNA cassette, PGK puro cassette
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Nov. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    189635-plasmid-map-sequence-id-414905

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  5. pLenti_CHyMErA.v2_U6(SpCas9_I-SceI)-(AsCas12a(dual-gRNA)_PacI-PacI)_PGK-puro

    Plasmid
    #189636
    Purpose
    Lentiviral expression of single SpCas9 and dual AsCas12a gRNAs for generating combinatorial CHyMErA.v2 3Cs libraries
    Depositor
    Manuel Kaulich
    Article
    Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.
    Insert
    hU6 Cas9-Cas12a-Cas12a gRNA cassette, PGK puro cassette
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Nov. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    189636-plasmid-map-sequence-id-414907

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  7. pJR85

    Plasmid
    #140095
    Purpose
    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region. BsmBI sites were removed to allow for programmed dual sgRNA library cloning.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA with cs1 in stem loop 2 of the constant region
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    140095-plasmid-map-sequence-id-292013

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  12. pGuide-P1-O1a (EL702)

    Plasmid
    #140040
    Purpose
    CRISPR DuMPLING negative control plasmid with probe/barcode P1 and negative control lacO1array spacer in the sgRNA. Also template for library PCRs.
    Depositor
    Johan Elf
    Article
    Camsund et al Nat Methods. 2020 Jan;17(1):86-92. doi:
    Insert
    barcode P1 and sgRNA lacO1 array
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    April 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    140040-plasmid-map-sequence-id-292294

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  13. pL452(hygro)-Sf3b1-K700K

    Plasmid
    #90426
    Purpose
    selectable HDR vector to introduce silent mutation (position 700) in mus Sf3b1 gene (for use with pL452-Sf3b1-K700E and sgSf3b1(T1) gRNA vectors enabling generation of hemizygous K700E mutation)
    Depositor
    Alex Minella
    Article
    Gupta et al Nucleic Acids Res. 2018 Nov 20. pii: 519
    Insert
    Sf3b1 - left and right homology arms (Sf3b1 Mouse)
    Use
    Homology-directed repair vector
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    90426-plasmid-map-sequence-id-190769

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  14. pAPH07

    Plasmid
    #234345
    Purpose
    Library-scale IPTG-inducible gRNA expression for Type II dCas9 in Synechococcus sp. PCC 7002, genomically integrated next to glpK
    Depositor
    Jerome Fox
    Article
    Hren et al Proc Natl Acad Sci U S A. 2025 Mar 25;12
    Insert
    Type II dCas9 sgRNA
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lacUV5
    Available Since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    234345-plasmid-map-sequence-id-470060

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  15. pAPH021

    Plasmid
    #234346
    Purpose
    Library-scale IPTG-inducible single transcript dual-gRNA expression for Type II dCas9 in Synechococcus sp. PCC 7002, genomically integrated next to glpK
    Depositor
    Jerome Fox
    Article
    Hren et al Proc Natl Acad Sci U S A. 2025 Mar 25;12
    Inserts
    Type II dCas9 sgRNA
    Type II dCas9 sgRNA
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lacUV5
    Available Since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    234346-plasmid-map-sequence-id-470058

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  16. LentiGuide Puro-P2A-EGFP

    Plasmid
    #137729
    Purpose
    For simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).
    Depositor
    Fredrik Wermeling
    Article
    Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Tags
    EGFP
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available Since
    Feb. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    137729-plasmid-map-sequence-id-272646

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Showing: 421 - 440 of 450 results