We narrowed to 1,020 results for: hug;

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pcDNA3.1(+)-blueAKAR(T/A)

Plasmid

#118471

Purpose

Negative-control mutant for blueAKAR biosensor.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

blueAKAR(T/A)

Tags

6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pR-CyOFP1

Plasmid

#228604

Purpose

Mammalian expression of CyOFP1, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

CyOFP1

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-blueCKAR

Plasmid

#118472

Purpose

cpBFP-based single-color biosensor for monitoring Protein Kinase C activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

blueCKAR

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pREDIT_Cas9n-MS2-BB_BbsI

Plasmid

#164804

Purpose

REDIT backbone for nickase Cas9n, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9n(D10A)-T2A-EGFP

Depositor

Le Cong

Article

Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pJS646

Plasmid

#183175

Purpose

Y2H vector compatible with pJS647 PYR1 insert

Depositor

Timothy Whitehead

Article

Beltran et al Nat Biotechnol. 2022 Jun 20. pii: 10.103

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pMXs-RAP2A-GFP

Plasmid

#86124

Purpose

Retroviral vector expressing RAP2A-GFP-IRES-Bsd

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

RAP2A (RAP2A Human)

Use

Retroviral

Tags

3xFLAG and GFP

Expression

Mammalian

Promoter

LTR

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT-DEST EVC2-Flag

Plasmid

#41005

Depositor

Rajat Rohatgi

Article

Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

Insert

Ellis van Creveld syndrome 2 (EVC2 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Nov. 14, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-RAB-EKARev(T/A)

Plasmid

#118475

Purpose

Negative-control mutant for RAB-EKARev biosensor.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

RAB-EKARev(T/A)

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ExRai-AktAR(T/A)

Plasmid

#118463

Purpose

Negative-control mutant for ExRai-AktAR biosensor.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-AktAR(T/A)

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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S1-Fc

Plasmid

#164220

Purpose

Expresses soluble, dimeric Fc-His fusion protein for the original SARS-CoV2 Spike S1 region, along with dTomato reporter

Depositor

Sriram Neelamegham

Article

Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1

Insert

Spike S1 domain (S SARS-CoV)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pEN-L1-PJ23119-PplpT-sfGFP-TrrfB-BsaI-Scaf-L2

Plasmid

#196250

Purpose

Plasmid for cloning of a CRISPR-Cas9 guide RNA gene under promoter PJ23119

Depositor

Bing Yang

Article

Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

Insert

attL1-GlpT-scGFP-attL2

Expression

Bacterial

Promoter

J23119 promoter

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pMXs-NDUFAF5-HA

Plasmid

#86125

Purpose

Retroviral vector expressing NDUFAF5-HA-IRES-Bsd

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

NDUFAF5 (NDUFAF5 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

LTR

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pCRU5-inGFPt

Plasmid

#60236

Purpose

HTLV-1 based transfer vector encoding gfp-turbo reporter gene interrupted by intron

Depositor

Dmitriy Mazurov

Article

Shunaeva et al J Virol. 2015 Oct 15;89(20):10591-601. d

Insert

inGFPt

Use

Retroviral

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR - Amplicon, BCR/ABL sgRNA 2

Plasmid

#70658

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backbone.

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

sgRNA against BCR/ABLamplicon found in CML-derived K562 cell line

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3-Lyn-RAB-EKARev

Plasmid

#118477

Purpose

ddRFP-based single-color ERK biosensor targeted to the plasma membrane.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

Lyn-RAB-EKARev

Tags

N-terminal targeting sequence from Lyn-kinase

Expression

Mammalian

Promoter

CMV

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pUCHR-inmCherry

Plasmid

#60239

Purpose

HIV-1 transfer vector that encodes mcherry reporter gene interrupted by intron

Depositor

Dmitriy Mazurov

Article

Shunaeva et al J Virol. 2015 Oct 15;89(20):10591-601. d

Insert

inmCherry

Use

Lentiviral

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pUCHR-inGFPt

Plasmid

#60237

Purpose

HIV-1 transfer vector that encodes gfp-turbo reporter gene interrupted by intron

Depositor

Dmitriy Mazurov

Article

Shunaeva et al J Virol. 2015 Oct 15;89(20):10591-601. d

Insert

inGFPt

Use

Lentiviral

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-sgPREX1

Plasmid

#86127

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting PREX1

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

gRNA targeting PREX1 (PREX1 )

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pCRU5-inmCherry

Plasmid

#60238

Purpose

HTLV-1 based transfer vector encoding mcherry reporter gene interrupted by intron

Depositor

Dmitriy Mazurov

Article

Shunaeva et al J Virol. 2015 Oct 15;89(20):10591-601. d

Insert

inmCherry

Use

Retroviral

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-ExRai-AKAR-NES

Plasmid

#118407

Purpose

Cytosol-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-AKAR-NES

Tags

Nuclear export signal (NES)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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