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Plasmid#63843PurposeThis plasmid is designed as a bacterial oxidative stress sensorDepositorInsertdps, mRFP1 and GFPmut3b
UseTagsmRFP1ExpressionBacterialMutationGene is transcriptionally fused to RFPPromoterAvailable sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
katG-RFP
Plasmid#63844PurposeThis plasmid is designed as a bacterial oxidative stress sensorDepositorInsertkatG, mRFP1 and GFPmut3b
UseTagsmRFP1ExpressionBacterialMutationGene is transcriptionally fused to RFPPromoterAvailable sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
AV0-hSyn-SV40pA
Plasmid#124965PurposeExpress gene of interest under human Syn promoterDepositorTypeEmpty backboneUseAAVTagsExpressionMutationPromoterAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
AV0-BWS-2.0
Plasmid#124976PurposeExpress gene of interest under EF1 promoterDepositorTypeEmpty backboneUseAAVTagsExpressionMutationPromoterAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
rpoS700-RFP
Plasmid#63839PurposeThis plasmid is designed as a bacterial ethanol sensorDepositorInsertrpoS, mRFP1 and GFPmut3b
UseTagsmRFP1ExpressionBacterialMutationGene is translationally fused to RFPPromoterAvailable sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL4.83(hRlucP/Puro)
Plasmid#212934PurposeRenilla luciferase+PEST vector for investigating faster response to transcriptional changesDepositorTypeEmpty backboneUseLuciferaseTagsExpressionMutationWTPromoterAvailable sinceJan. 10, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
rrsA-RFP
Plasmid#63837PurposeThis plasmid is designed as a bacterial nutrient sensorDepositorInsertrrsA, mRFP1 and GFPmut3b
UseTagsmRFP1ExpressionBacterialMutationGene is transcriptionally fused to RFPPromoterAvailable sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
gpmM-RFP
Plasmid#63841PurposeThis plasmid is designed as a bacterial carbon flux sensorDepositorInsertgpmM, mRFP1 and GFPmut3b
UseTagsmRFP1ExpressionBacterialMutationGene is translationally fused to RFPPromoterAvailable sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
Plasmid#140082PurposeEntry cloning vector for in vitro transcription or expression of SpCas9 sgRNAs from a T7 promoterDepositorInsertpT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
UseIn vitro transcription (mrna synthesis)TagsExpressionBacterialMutationPromoterT7Available sinceOct. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFGC-pcoCas9
Plasmid#52256PurposeA binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant expression of Cas9 and multiple cloning sites for insertion of guide RNADepositorInsert35SPPDK:pcoCas9:NOS cassette
UseCRISPR; Plant expressionTagsExpressionMutationPromoterconstitutive 35SPPDK promoterAvailable sinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_SARS-COV-2_S
Plasmid#169402PurposeCell-free/mammalian expression vector for SARS-COV-2 virus S with N-term eGFPDepositorInsertSARS-COV-2 _S (S SARS-Cov2)
UseCell-free expression vectorTagsHis and eGFPExpressionMutationPromoterT7- CMV/CAGAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET15b His6-ecDHFR (WT)
Plasmid#73188Purposebacterial express ecDHFR protein for washing out TMP ligand for pBMN DHFR(DD)-YFP (Plasmid #29325) and pBMN YFP-DHFR(DD) (Plasmid #29326)DepositorInsertfolA dihydrofolate reductase (folA E. coli)
UseTagsHis 6XExpressionBacterialMutationnonePromoterAvailable sinceFeb. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
R619-X67-527: His6-SARS-CoV-2 N
Plasmid#170204PurposeE. coli expression of SARS-CoV-2 nucleocapsid proteinDepositorInsertSARS-CoV-2 N (N SARS-CoV-2)
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA3_SARS-COV-2_RBD
Plasmid#169405PurposeCell-free/mammalian expression vector for SARS-COV-2 RBD domain of S protein with C-term mCherryDepositorInsertSARS-COV-2 _RBD (S SARS-Cov2)
UseCell-free expression vectorTagsmCherryExpressionMutationPromoterT7- CMV/CAGAvailable sinceJuly 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMCSG53_Nsp3_Unique
Plasmid#167258PurposeBacterial expression of Nsp3 Macrodomain 2 and Macrodomain 3 (residues 413-676)DepositorInsertNsp3 (residues 413-676) (ORF1ab SARS-CoV-2)
UseTagsHis6-TEV siteExpressionBacterialMutationnonePromoterAvailable sinceApril 27, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pmCellFree_KA1_SARS-COV-2_ORF10
Plasmid#169398PurposeCell-free/mammalian expression vector for SARS-COV-2 virus ORF10 with N-term eGFPDepositorInsertSARS-COV-2 _ORF10 (ORF10 SARS-Cov2)
UseCell-free expression vectorTagsHis and eGFPExpressionMutationPromoterT7- CMV/CAGAvailable sinceJuly 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMTF1.1.0-gDNA
Plasmid#113797PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor MTF1DepositorInsertMTF1 (MTF1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAIO-Ef1a-PE2-GFP:KCNQ2-C201R
Plasmid#185060PurposeEf1a driven PE2 plasmid with pegRNA for editing C201R mutation in KCNQ2 gene. See Addgene plasmid #184445DepositorInsertU6:pegRNA:scaffold:PBS+RT template
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-SaCas9-U6-sgSlc6a3
Plasmid#159902PurposeMutagenesis of Slc6a3DepositorInsertSlc6a3 (Slc6a3 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_SARS-COV-2_M
Plasmid#169400PurposeCell-free/mammalian expression vector for SARS-COV-2 virus M with N-term eGFPDepositorInsertSARS-COV-2 _M (M SARS-Cov2)
UseCell-free expression vectorTagsHis and eGFPExpressionMutationPromoterT7- CMV/CAGAvailable sinceJuly 2, 2021AvailabilityAcademic Institutions and Nonprofits only