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pMT-EGFP-CTAP-PrP-SBP_W

Plasmid

#147835

Purpose

Insect Expression of EGFP

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

EGFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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Hy_pMtnA UTR delta 21-55 eGFP SV40

Plasmid

#132653

Purpose

Expresses an eGFP mRNA from the Drosophila Metallothionein A promoter that has 20 nt of the MtnA 5' UTR

Depositor

Jeremy Wilusz

Article

Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

Insert

eGFP

Use

Tags

Expression

Insect

Mutation

Promoter

MtnA

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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Hy_pMtnA UTR delta 41-55 eGFP SV40

Plasmid

#132652

Purpose

Expresses an eGFP mRNA from the Drosophila Metallothionein A promoter that has 40 nt of the MtnA 5' UTR

Depositor

Jeremy Wilusz

Article

Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

Insert

eGFP

Use

Tags

Expression

Insect

Mutation

Promoter

MtnA

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT mir-1-1 reporter cel-mir-50

Plasmid

#46740

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-mir-50

Use

RNAi

Tags

His and hsa-mir-1-1

Expression

Insect

Mutation

Promoter

Metallothionein (MT)

Available since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT mir-1-1 reporter cel-lsy-6

Plasmid

#46738

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-lsy-6

Use

RNAi

Tags

His and hsa-mir-1-1

Expression

Insect

Mutation

Promoter

Metallothionein (MT)

Available since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT mir-1-1 reporter cel-lin-4

Plasmid

#46737

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-lin-4

Use

RNAi

Tags

His and hsa-mir-1-1

Expression

Insect

Mutation

Promoter

Metallothionein (MT)

Available since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT Draper-delta ITAM-GFP

Plasmid

#126655

Purpose

Inducible expression of Draper-delta ITAM-GFP mutant in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Draper with ITAM Tyr residues mutated to Ala

Use

Tags

Expression

Insect

Mutation

Cytoplasmic ITAM Tyr residues mutated to Phe

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pMT FRB-Draper INT-GFP

Plasmid

#126665

Purpose

Inducible expression of a synthetic FRB-Draper receptor in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

FRB-Draper INT domain

Use

Tags

Expression

Insect

Mutation

K986N- see depositor comments below

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pMT FRB-Draper INT-mCherry

Plasmid

#126666

Purpose

Inducible expression of a synthetic FRB-Draper receptor in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

FRB-Draper INT domain mCherry

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
Hy_pMT Laccase2 MCS-ZKSCAN1 548-1047

Plasmid

#69886

Purpose

Expresses a miniature version of the ZKSCAN1 circular RNA in Drosophila

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ZKSCAN1 (stw, ZKSCAN1 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Metallothionein Promoter (pMT)

Available since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pMT1-13X-gRNA1-2(AtUBI10)-MTAP-LUC

Plasmid

#234370

Purpose

T-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs

Depositor

Michael Smanski

Article

Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

Inserts

NbGP41-sfGFP-VP64-GB1
Luciferase
dCas9-13XGP41
gRNA 1-1 and gRNA 1-2

Use

Synthetic Biology

Tags

GB1, GP41, and sfGFP

Expression

Plant

Mutation

Promoter

Arabidopsis U6, Arabidopsis Ubi10, and MTAP1 (syn…

Available since

April 17, 2025

Availability

Academic Institutions and Nonprofits only

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pMT1-13X-NOG(AtUBI10)-MTAP-LUC

Plasmid

#234371

Purpose

T-DNA vector for expression of the the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs, no gRNA included (NOG)

Depositor

Michael Smanski

Article

Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

Inserts

NbGP41-sfGFP-VP64-GB1
Luciferase
dCas9-13XGP41

Use

Synthetic Biology

Tags

GB1, GP41, and sfGFP

Expression

Plant

Mutation

Promoter

Arabidopsis Ubi10 and MTAP1 (synthetic)

Available since

April 17, 2025

Availability

Academic Institutions and Nonprofits only

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pMT1-24X-gRNA1-2(AtUBI10)-MTAP-LUC

Plasmid

#234372

Purpose

T-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs

Depositor

Michael Smanski

Article

Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

Inserts

NbGP41-sfGFP-VP64-GB1
Luciferase
dCas9-24XGP41
gRNA 1-1 and gRNA 1-2

Use

Synthetic Biology

Tags

GB1, GP41, and sfGFP

Expression

Plant

Mutation

Promoter

Arabidopsis U6, Arabidopsis Ubi10, and MTAP1 (syn…

Available since

April 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT-CTAP-PrP-SBP-DmCaf40-N63AF65AC69YV76Y_W

Plasmid

#147833

Purpose

Insect Expression of DmCaf40-N63AF65AC69YV76Y

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmCaf40-N63AF65AC69YV76Y (Rcd-1 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pMT-CTAP-PrP-SBP-DmCaf40-NFCVYAtoAAYYAF_W

Plasmid

#147834

Purpose

Insect Expression of DmCaf40-NFCVYAtoAAYYAF

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmCaf40-NFCVYAtoAAYYAF (Rcd-1 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Hy_pMT Laccase2 MCS-Laccase2 608-1097

Plasmid

#69890

Purpose

Expresses the Drosophila laccase2 exon 2 circular RNA

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

Laccase2 (stw Fly)

Use

Tags

Expression

Insect

Mutation

L158I in laccase2

Promoter

Metallothionein Promoter (pMT)

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Laccase2 MCS-ZKSCAN1 548-847

Plasmid

#69885

Purpose

Expresses a miniature version of the ZKSCAN1 circular RNA in Drosophila

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ZKSCAN1 (stw, ZKSCAN1 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Metallothionein Promoter (pMT)

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT mir-1-1 reporter hsa-mir-205

Plasmid

#46744

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

hsa-mir-205 (MIR205 Human)

Use

RNAi

Tags

His and hsa-mir-1-1

Expression

Insect

Mutation

Promoter

Metallothionein (MT)

Available since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT mir-1-1 reporter hsa-mir-128-1

Plasmid

#46743

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

hsa-mir-128-1 (MIR128-1 Human)

Use

RNAi

Tags

His and hsa-mir-1-1

Expression

Insect

Mutation

Promoter

Metallothionein (MT)

Available since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT mir-1-1 reporter cel-mir-240

Plasmid

#46742

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-mir-240

Use

RNAi

Tags

His and hsa-mir-1-1

Expression

Insect

Mutation

Promoter

Metallothionein (MT)

Available since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge