We narrowed to 22,191 results for: his

1. pET30-T7-lacO-sfGFP

   Plasmid

   #180756

   Purpose

   pET30 vector missing N-terminal His tag with sfGFP-S-tag inserted between the Bgl II and T7 terminator

   Depositor

   Michael Black

   Article

   Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

   Insert

   superfolder green fluorescent protein
   

   Use

   Tags

   strep tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28_canine-Caspase-1/4_enzymatic-domain

   Plasmid

   #183383

   Purpose

   Overexpression of catalytic domain of inflammatory caspase in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   Caspase-1/4 (AA130-404) (CASP4 Canis lupus familiaris)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEcCas5

   Plasmid

   #170090

   Purpose

   expresses E. coli type I-E Cas5

   Depositor

   Chase Beisel

   Article

   Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

   Insert

   E. coli type I-E Cas5
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEcCas7

   Plasmid

   #170092

   Purpose

   expresses E. coli type I-E Cas7

   Depositor

   Chase Beisel

   Article

   Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

   Insert

   E. coli type I-E Cas7
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEcCas8

   Plasmid

   #170093

   Purpose

   expresses E. coli type I-E Cas8

   Depositor

   Chase Beisel

   Article

   Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

   Insert

   E. coli type I-E Cas8
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEcCse2

   Plasmid

   #170094

   Purpose

   expresses E. coli type I-E Cse2

   Depositor

   Chase Beisel

   Article

   Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

   Insert

   E. coli type I-E Cse2
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Lmajor_gp63_28_0570_WT

   Plasmid

   #171642

   Purpose

   Expression of wildtype L. major glcyoprotein-63 from chromosome 28 in mammalian cells

   Depositor

   Dennis Ko

   Article

   Antonia et al PLoS Negl Trop Dis. 2021 Oct 28;15(10):e

   Insert

   LmjF.28.0570
   

   Use

   Tags

   Myc-His

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Lmajor_gp63_31_2000_WT

   Plasmid

   #171643

   Purpose

   Expression of wildtype L. major glcyoprotein-63 from chromosome 31 in mammalian cells

   Depositor

   Dennis Ko

   Article

   Antonia et al PLoS Negl Trop Dis. 2021 Oct 28;15(10):e

   Insert

   LmjF.31.2000
   

   Use

   Tags

   Myc-His

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBAD-MtrxRFP2

   Plasmid

   #172323

   Purpose

   fluorescent biosensor for the redox of mitochondrial specific thioredoxin (Trx2)

   Depositor

   Huiwang Ai

   Article

   Pang et al Biochemistry. 2022 Mar 1;61(5):377-384.

   Insert

   human thioredoxin 2, fused with a redox-sensitive RFP through Gly-Ser-rich linker
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available Since

   Aug. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRSETA_crG18

    Plasmid

    #172930

    Purpose

    Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 18x GSG

    Depositor

    Arnold Boersma

    Article

    Liu et al Biophys J. 2017 May 9;112(9):1929-1939.

    Insert

    CrowdingSensorG18
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pACYCDuet_DL121_WTTS

    Plasmid

    #171954

    Purpose

    Selection vector for chimeric fusion of ecDHFR and asLOV2.

    Depositor

    Kimberly Reynolds

    Article

    McCormick et al Elife. 2021 Jun 16;10. pii: 68346. doi:

    Inserts

    Fusion of ecDHFR and asLOV2 at position 121 of DHFR
    Thymidylate Synthase
    

    Use

    Tags

    8-His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBAD-wtBlc

    Plasmid

    #168255

    Purpose

    wild‐type E. coli lipocalin Blc without signal peptide

    Depositor

    Jens Meiler

    Article

    Bozhanova et al FEBS Lett. 2021 Jan;595(2):206-219. doi:

    Insert

    Lipocalin Blc
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available Since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET11a-N-DiB2

    Plasmid

    #168472

    Purpose

    N-fragment of the DiB2‐split protein. This plasmid needs to be co-transformed with Plasmid 168257: pMRBad-C-wtBlc to obtain DiB2‐split protein

    Depositor

    Jens Meiler

    Article

    Bozhanova et al Sci Rep. 2020 Jul 6;10(1):11049. doi: 10

    Insert

    N-fragment of DiB2
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBEL2298

    Plasmid

    #164603

    Purpose

    Expression and Complementation plasmid for MlaD (A17F, A20F, V24F)

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

    Insert

    MlaFEDCB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    MlaF-(6xHis-MlaE)-MlaD(Ala17Phe, Ala20Phe, Val24P…

    Promoter

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBEL1647

    Plasmid

    #155148

    Purpose

    Expresses MlaF 1-246, tagless

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

    Insert

    mlaF(1-246)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    araBAD

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBEL1244

    Plasmid

    #155138

    Purpose

    Expresses MlaFED operon in E. coli

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

    Insert

    mlaFE-[6xHis-2xQH-TEV-mlaD]-mlaC operon
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    araBAD

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBEL1266

    Plasmid

    #155142

    Purpose

    Expresses MlaF, tagless, araBAD promoter

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

    Insert

    MlaF
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    araBAD

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pVW294

    Plasmid

    #139491

    Purpose

    Insertion of the pGRE2 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiae

    Depositor

    Serge Pelet

    Article

    Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

    Insert

    pGRE2 24xPP7sl
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    WT

    Promoter

    pGRE2

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pVW295

    Plasmid

    #139492

    Purpose

    Insertion of the pALD3 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiae

    Depositor

    Serge Pelet

    Article

    Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

    Insert

    pALD3 24xPP7sl
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    WT

    Promoter

    pALD3

    Available Since

    June 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSP266

    Plasmid

    #139488

    Purpose

    Insertion of the pCTT1 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiae

    Depositor

    Serge Pelet

    Article

    Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

    Insert

    pCTT1 24xPP7sl
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    WT

    Promoter

    pCTT1

    Available Since

    June 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSP264

    Plasmid

    #139487

    Purpose

    Insertion of the pSTL1 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiae

    Depositor

    Serge Pelet

    Article

    Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

    Insert

    pSTL1 24xPP7sl
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    WT

    Promoter

    pSTL1

    Available Since

    June 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pVW200

    Plasmid

    #139489

    Purpose

    Insertion of the pGPD1 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiae

    Depositor

    Serge Pelet

    Article

    Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

    Insert

    pGPD1 24xPP7sl
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    WT

    Promoter

    pGPD1

    Available Since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBAD-Citroff1-R251

    Plasmid

    #134307

    Purpose

    Bacterial expression of an inverse-response citrate sensor variant with medium binding affinity

    Depositor

    Robert Campbell

    Article

    Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

    Insert

    Citroff1 R251A
    

    Use

    Tags

    6-His tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available Since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBAD-Citroff1-S245

    Plasmid

    #134302

    Purpose

    Bacterial expression of an inverse-response citrate sensor variant with medium binding affinity

    Depositor

    Robert Campbell

    Article

    Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

    Insert

    Citroff1 S245A
    

    Use

    Tags

    6-His tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available Since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pWDY1228

    Plasmid

    #138594

    Purpose

    Expresses NatL2-R156K in E.coli

    Depositor

    James Naismith

    Article

    Song et al Angew Chem Int Ed Engl. 2020 Jan 5. doi:

    Insert

    phenylacetate-coenzyme A ligase
    

    Use

    Tags

    6-His

    Expression

    Mutation

    changed Arginine 156 to Lysine

    Promoter

    T7

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pWDY1211

    Plasmid

    #138591

    Purpose

    Expresses NatL2 in E.coli

    Depositor

    James Naismith

    Article

    Song et al Angew Chem Int Ed Engl. 2020 Jan 5. doi:

    Insert

    phenylacetate-coenzyme A ligase
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET-Himar-dCas9-mammalian

    Plasmid

    #138414

    Purpose

    Protein purification of Himar-dCas9 with 3x-FLAG, SV40 NLS, and 6x-His tag

    Depositor

    Harris Wang

    Article

    Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

    Insert

    Himar-dCas9
    

    Use

    Tags

    3xFLAG, 6xHis, and SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRSETA-Prp28

    Plasmid

    #111285

    Purpose

    Prp28 expression plasmid

    Depositor

    Christine Guthrie

    Article

    Price et al RNA. 2014 Jan;20(1):46-60. doi: 10.1261/

    Insert

    PRP28
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCMV-M-RAN-H2A2B

    Plasmid

    #106420

    Purpose

    M-RAN-H2A2B (RANbody H2A2B::Sm-MYCtag)

    Depositor

    Joshua Sanes

    Article

    Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

    Insert

    M-RAN-H2A2B
    

    Use

    Tags

    HA, His, and Multiple MYC

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMV-Y-RAN-H2A2B

    Plasmid

    #106421

    Purpose

    Y-RAN-H2A2B (RANbody H2A2B::Chicken IgY)

    Depositor

    Joshua Sanes

    Article

    Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

    Insert

    Y-RAN-H2A2B
    

    Use

    Tags

    HA and His

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. p415 Ret2LD2a Q146F

    Plasmid

    #75259

    Purpose

    Q146F mutation

    Depositor

    Blanche Schwappach

    Article

    Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

    Insert

    Ret2LD2a
    

    Use

    Tags

    His

    Expression

    Yeast

    Mutation

    Q146F

    Promoter

    Met25

    Available Since

    Dec. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. p415 Ret2LD2a T158K

    Plasmid

    #75262

    Purpose

    T158K mutation

    Depositor

    Blanche Schwappach

    Article

    Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

    Insert

    Ret2LD2a T158K
    

    Use

    Tags

    His

    Expression

    Yeast

    Mutation

    Promoter

    Met25

    Available Since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. p415 Ret2LD2a K153Q

    Plasmid

    #75261

    Purpose

    K153Q mutation

    Depositor

    Blanche Schwappach

    Article

    Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

    Insert

    Ret2LD2a K153Q
    

    Use

    Tags

    His

    Expression

    Yeast

    Mutation

    Promoter

    Met25

    Available Since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. p415 Ret2LD2a N152T

    Plasmid

    #75260

    Purpose

    N152T mutation

    Depositor

    Blanche Schwappach

    Article

    Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

    Insert

    Ret2LD2a N152T
    

    Use

    Tags

    His

    Expression

    Yeast

    Mutation

    Promoter

    Met25

    Available Since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. JARID1BA-c539

    Plasmid

    #79643

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    JARID1B (KDM5B Human)
    

    Use

    Baculovirus expression

    Tags

    6x His and TEV cleavage site

    Expression

    Mutation

    Promoter

    polyhedrin

    Available Since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

36. TbSIR2RP1

    Plasmid

    #67242

    Purpose

    Determine its activity and specificity

    Depositor

    Cynthia Wolberger

    Article

    Fahie et al FEBS J. 2009 Dec;276(23):7159-76. doi: 1

    Insert

    TbSIR2RP1
    

    Use

    Tags

    MBP, TEV, Thrombin, HIS, and none

    Expression

    Bacterial

    Mutation

    Promoter

    p-lac

    Available Since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

37. pJK560

    Plasmid

    #71585

    Purpose

    Produces E. coli acetyl-CoA:oxalate CoA-transferase (yfdE) with Arg232>His mutant (YfdE-R232H)

    Depositor

    T. J. Kappock

    Article

    Mullins et al PLoS One. 2013 Jul 23;8(7):e67901. doi:

    Insert

    acetyl-CoA:oxalate CoA-transferase (yfdE )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changed arginine-232 to histidine

    Promoter

    T7 promoter

    Available Since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCellFree_G03 TFAP4

    Plasmid

    #67073

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TFAP4 (TFAP4 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    P338A

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

39. c7-87/199_HLL

    Plasmid

    #63122

    Purpose

    Mm-cpn cysteine double mutant for crosslinking

    Depositor

    Tanja Kortemme

    Article

    Hoersch et al Nat Nanotechnol. 2013 Dec;8(12):928-32.

    Insert

    Mm-cpn c7_87/199_HLL (NEWENTRY )
    

    Use

    Tags

    6x HIS-tag

    Expression

    Bacterial

    Mutation

    C140S-C237S-C286A-C359T-C393A-C470S-C484S-K87C-S1…

    Promoter

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. c7-132/202_HLL

    Plasmid

    #63123

    Purpose

    Mm-cpn double cysteine mutant for crosslinking

    Depositor

    Tanja Kortemme

    Article

    Hoersch et al Nat Nanotechnol. 2013 Dec;8(12):928-32.

    Insert

    Mm-cpn c7_132/202_HLL (NEWENTRY )
    

    Use

    Tags

    6x HIS-tag

    Expression

    Bacterial

    Mutation

    C140S-C237S-C286A-C359T-C393A-C470S-C484S-Q132C-S…

    Promoter

    Available Since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge