Addgene

We narrowed to 23,141 results for: Nov

Showing: 10951 - 11000 of 23141 results

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pAFMW-Nbr[N1-383]

Plasmid

#188586

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler N-terminal domain

Use

Tags

FLAG-Myc

Expression

Insect

Mutation

deleted first ATG

Promoter

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pACE-GIRK4-mC-S

Plasmid

#172427

Purpose

Expression of full-length, human GIRK4 with C-terminal mCherry StrepTag II

Depositor

Arthur Laganowsky

Article

Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

Insert

GIRK4 (KCNJ5 Human)

Use

Tags

mCherry Streptag II

Expression

Insect

Mutation

Promoter

Available since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pLL5.0 shFmnl2-EGFP

Plasmid

#187255

Purpose

Lentiviral expression of shRNA targeting Fmnl2

Depositor

Alpha Yap

Article

Katsuno-Kambe et al Biol Cell. 2021 Feb;113(2):107-117. doi:

Insert

ShRNA for Fmnl2 (Fmnl2 Mouse)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

U6

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_316

Plasmid

#180547

Purpose

Entry vector containing sfGFP

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_344

Plasmid

#180548

Purpose

Entry vector containing cjBlue

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

cjBlue

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-ammB3

Plasmid

#167815

Purpose

Expresses His6-tagged AmmB3 in E. coli

Depositor

Wilfred van der Donk

Article

Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

Insert

AmmB3

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-ammC1

Plasmid

#167816

Purpose

Expresses His6-tagged AmmC1 in E. coli

Depositor

Wilfred van der Donk

Article

Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

Insert

AmmC1

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_345

Plasmid

#180550

Purpose

Entry vector containing eforRed

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

eforRed

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_036

Plasmid

#180558

Purpose

Entry vector containing L3S1P13 terminator

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

L3S1P13 terminator

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_009

Plasmid

#180518

Purpose

Empty backbone for D1.1 cloning, contains LacZ dropout

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_137

Plasmid

#180521

Purpose

Empty backbone for D1.2 cloning, contains sfGFP dropout

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_010

Plasmid

#180519

Purpose

Empty backbone for D1.2 cloning, contains LacZ dropout

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_040

Plasmid

#180562

Purpose

Entry vector containing L3S2P24 terminator

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

L3S2P24 terminator

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_048

Plasmid

#180564

Purpose

Entry vector containing L3S1P00

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

L3S1P00_E1.4b

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_029

Plasmid

#180542

Purpose

Entry vector containing RBS BBa_B0035

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

RBS BBa_B0035

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_035

Plasmid

#180557

Purpose

Entry vector containing L3S1P00 terminator

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

L3S1P00 terminator

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_033

Plasmid

#180545

Purpose

Entry vector containing LuxR

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

LuxR

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_037

Plasmid

#180559

Purpose

Entry vector containing L3S1P22 terminator

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

L3S1P22 terminator

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_003

Plasmid

#180541

Purpose

Entry vector containing RBS BBa_B0034

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

RBS BBa_B0034

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_016

Plasmid

#180523

Purpose

Empty backbone for D2.2b cloning, contains LacZ dropout

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_015

Plasmid

#180522

Purpose

Empty backbone for D2.2a cloning, contains LacZ dropout

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_039

Plasmid

#180561

Purpose

Entry vector containing L3S2P21 terminator

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

L3S2P21 terminator

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_038

Plasmid

#180560

Purpose

Entry vector containing L3S2P11 terminator

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

L3S2P11 terminator

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
1098E=TI-pgSIT[tra,bTub,Hasp70Bb-Cas9]

Plasmid

#149427

Purpose

The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.

Depositor

Omar Akbari

Article

Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

Inserts

U6.3-gRNAs[TraB, bTub]
Hsp70Bb-Cas9-T2A-eGFP-p10

Use

CRISPR

Tags

eGFP

Expression

Insect

Mutation

Promoter

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-CeAIN2_254-400-V5His6_R

Plasmid

#147407

Purpose

Insect Expression of CeAIN2_254-400

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400

Use

Tags

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Promoter

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-H2B-Sirius

Plasmid

#175837

Purpose

Express Sirius with nucleus-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Sirius with nucleus-targeting sequence

Use

Tags

H2B (human histone 2B)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFX-iRFP-Rab5

Plasmid

#174456

Purpose

Express iRFP with early endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

iRFP with early endosome-targeting sequence

Use

Tags

small GTPase (Rab5)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFX-SECFP-Sec61β

Plasmid

#174465

Purpose

Express SECFP with endoplasmic reticulum-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

SECFP with endoplasmic reticulum-targeting sequence

Use

Tags

Sec61 subunit beta

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFX-iRFP-KRasCT

Plasmid

#175836

Purpose

Express iRFP with plasma membrane-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

iRFP with plasma membrane-targeting sequence

Use

Tags

C-terminal hypervariable region of K-Ras 4B

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFX-Tom20-Sirius

Plasmid

#174184

Purpose

Express Sirius with mitochondria-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Sirius with mitchondria-targeting sequence

Use

Tags

the mitochondria-targeting sequence of TOM20

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFX-Sirius-Sec61β

Plasmid

#174464

Purpose

Express Sirius with endoplasmic reticulum-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Sirius with endoplasmic reticulum-targeting sequence

Use

Tags

Sec61 subunit beta

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFX-GalT-Sirius

Plasmid

#174469

Purpose

Express Sirius with golgi apparatus-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Sirius with golgi apparatus-targeting sequence

Use

Tags

β1,4-galactose transferase

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFX-iRFP-Rab7

Plasmid

#174459

Purpose

Express iRFP with late endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

iRFP with late endosome-targeting sequence

Use

Tags

small GTPase (Rab7)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
THI ONLY pALD4 T-

Plasmid

#161497

Purpose

Non activation and non targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T-_gRNA_MS2

Depositor

Diethard Mattanovich, Brigitte Gasser

Article

Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

Inserts

dCAS9
MS2 (non activation control NAC)
gRNA (Non targeting control NTC)

Use

Tags

Expression

Yeast

Mutation

Promoter

pALD4, pPOR1, and pTEF2

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-optimized E6AP F727D

Plasmid

#165101

Purpose

ecoli optimized expression of a GST fusion of Human E6AP F727D

Depositor

Arthur Haas

Article

Ronchi et al J Biol Chem. 2014 Jan 10;289(2):1033-48.

Insert

E6AP F727D (UBE3A Human)

Use

Tags

GST from plasmid.

Expression

Bacterial

Mutation

codon optimized for E. coli expression

Promoter

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJS249

Plasmid

#134146

Purpose

Bacterial expression of human Ska1 microtubule binding domain (MTBD)

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

pHis-Precission-human Ska1 MTBD (SKA1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDK616

Plasmid

#134202

Purpose

Bacterial expression of human Ska1 microtubule binding domain (MTBD) mutant

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

His-Precission-human Ska1 MTBD (SKA1 Human)

Use

Tags

Expression

Bacterial

Mutation

K223A K226A

Promoter

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJS263

Plasmid

#134141

Purpose

Bacterial expression of C. elegans Ska1 microtubule binding domain (MTBD)

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

Ska1 MTBD-His (Y106G6H.15 Nematode)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pIW087

Plasmid

#134128

Purpose

Bacterial expression of GFP-Ska1 and Ska2

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

His-GFP-human Ska1 ; human Ska2

Use

Tags

GFP

Expression

Bacterial

Mutation

K183A K184A

Promoter

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pIW090

Plasmid

#134129

Purpose

Bacterial expression of GFP-Ska1 and Ska2

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

His-GFP-human Ska1 ; human Ska2

Use

Tags

GFP

Expression

Bacterial

Mutation

K223A K226A

Promoter

Available since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pIW070

Plasmid

#134138

Purpose

Retroviral expression of GFP-Ska1

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

GFP-human Ska1 delta MTBD CRISPR/Cas9 hardened (SKA1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

residues 1-131, CRISPR resistant

Promoter

Available since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pIW093

Plasmid

#134137

Purpose

Retroviral expression of GFP-Ska1

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

GFP-human Ska1 CRISPR/Cas9 hardened (SKA1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

CRISPR resistant, R155A

Promoter

Available since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pIW082

Plasmid

#134136

Purpose

Retroviral expression of GFP-Ska1

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

GFP-human Ska1 CRISPR/Cas9 hardened (SKA1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

CRISPR resistant, R236A

Promoter

Available since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pIW088

Plasmid

#134135

Purpose

Retroviral expression of GFP-Ska1

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

GFP-human Ska1 CRISPR/Cas9 hardened (SKA1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

CRISPR resistant, K223A K226A

Promoter

Available since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pIW085

Plasmid

#134134

Purpose

Retroviral expression of GFP-Ska1

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

GFP-human Ska1 CRISPR/Cas9 hardened (SKA1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

CRISPR resistant, K183A K184A

Promoter

Available since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
phNFL-P22S

Plasmid

#132591

Purpose

Expresses human NFL containing the Charcot-Marie-Tooth disease type 2E P22S mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

NEFL (NEFL Human)

Use

Tags

Expression

Mammalian

Mutation

64C>A, 65C>G; Pro22Ser

Promoter

CMV

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK_ToeRepG2_N64_trigger

Plasmid

#132746

Purpose

T7 RNAP-driven expression of second-generation toehold repressor index 64 trigger RNA

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

ToeRepG2_N64_trigger

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG_PluxB_ToeRep_N01_trigger

Plasmid

#132736

Purpose

Inducible expression of first-generation toehold repressor index 1 trigger RNA via the PluxB promoter

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

PluxB_ToeRep_N01_trigger

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG_ToeRep_N09_trigger

Plasmid

#132740

Purpose

T7 RNAP-driven expression of first-generation toehold repressor index 9 trigger RNA

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

ToeRep_N09_trigger

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pYZ_PluxB_3WJrep_N19_trigger

Plasmid

#132738

Purpose

Inducible expression of 3WJ repressor index 19 trigger RNA via the PluxB promoter

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

PluxB_3WJrep_N19_trigger

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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