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pAAV-hSyn-mCherry-Plxnd1 sesRNA-2a-msFlag-2a-tTA2-WPRE

Plasmid

#239032

Purpose

Expression of mCherry, sesRNA in mammalian cells, with msFlag and tTA2 as efRNA.

Depositor

Josh Huang

Article

Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

Insert

sesRNA for mouse PlxnD1 (Plxnd1 Mouse)

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Aug. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.3 N-MYC ES8L3

Plasmid

#227881

Purpose

Used for transfection of cells for expression of C-MYC ES8L3

Depositor

Sheng-cai Lin

Article

Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

Insert

ES8L3 (Eps8l3 Mouse)

Tags

MYC

Expression

Mammalian

Available Since

June 27, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-mEGFP-hCTNNA1_DM1-3_WT-ABD

Plasmid

#234553

Purpose

CTNNA1 M-domain deletion mutant

Depositor

Cara Gottardi

Article

Wang et al J Cell Biol. 2025 Mar 3;224(3):e20230812

Insert

mEGFP:hCTNNA1_DM-domain (CTNNA1 Human)

Use

Lentiviral

Tags

mEGFP

Mutation

Deletion of aa 273-635

Promoter

CMV

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-mEGFP-hCTNNA1_L436P

Plasmid

#234559

Purpose

CTNNA1 eye phenotype via forward genetic screen

Depositor

Cara Gottardi

Article

Wang et al J Cell Biol. 2025 Mar 3;224(3):e20230812

Insert

mEGFP:hCTNNA1_L436P (CTNNA1 Human)

Use

Lentiviral

Tags

mEGFP

Mutation

L436P

Promoter

CMV

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pAG8Ha-FLI1_DBD_R2L2

Plasmid

#188049

Purpose

Expresses His8-FLI1_DBD_R2L2(276-399_R337L_R340L_F362A) with a TEV site for removal of the His8 tag.

Depositor

David Libich

Article

Selig et al Proc Natl Acad Sci U S A. 2025 May 20;12

Insert

FLI1 (FLI1 Human)

Tags

His8

Expression

Bacterial

Mutation

deleted amino acids 1-275, 400-452, mutated R337L…

Promoter

T7

Available Since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAG8Ha-FLI1_DBD_deltaAlpha4

Plasmid

#188050

Purpose

Expresses His8-FLI1_DBD_deltaAlpha4(276-361) with a TEV site for removal of the His8 tag.

Depositor

David Libich

Article

Selig et al Proc Natl Acad Sci U S A. 2025 May 20;12

Insert

FLI1 (FLI1 Human)

Tags

His8

Expression

Bacterial

Mutation

deleted amino acids 1-275, 362-452

Promoter

T7

Available Since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAG8Ha-FLI1_DBD(276-399_F362A)

Plasmid

#188047

Purpose

Expresses His8-FLI1_DBD(276-399_F362A) with a TEV site for removal of the His8 tag.

Depositor

David Libich

Article

Selig et al Proc Natl Acad Sci U S A. 2025 May 20;12

Insert

FLI1 (FLI1 Human)

Tags

His8

Expression

Bacterial

Mutation

deleted amino acids 1-275, 400-452, mutated F362A

Promoter

T7

Available Since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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TFORF3379

Plasmid

#144855

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ACTL6A (ACTL6A Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-L928A-M929A

Plasmid

#233065

Purpose

Expresses tethered-agonist dead mouse ADGRL3-L928A-M929A (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide.

Depositor

Jonathan Javitch

Article

Perry-Hauser et al J Biol Chem. 2025 Jan 9:108174. doi: 10.

Insert

ADGRL3 (Adgrl3 Mouse)

Tags

ALFA and HiBiT

Expression

Mammalian

Mutation

Leucine 928 to Alanine; Methionine 929 to Alanine

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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3xFLAG-DGKδ2

Plasmid

#226506

Purpose

Express N-terminal three tandem FLAG epitope tags, followed by an enterokinase cleavage site and human DGKD gene

Depositor

Chiaki Murakami, Fumio Sakane

Article

Murakami et al J Biol Chem. 2020 Mar 6;295(10):2932-294

Insert

DGKD (DGKD Human)

Tags

Three tandem FLAG epitope tag and enterokinase cl…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pSuper Retro GFP DGK zeta mouse

Plasmid

#224577

Purpose

Negative Control for downregulation of the expression of human DGK zeta in human cells

Depositor

Isabel Mérida

Article

Torres-Ayuso et al Oncotarget. 2014 Oct 30;5(20):9710-26. d

Insert

sh RNAi Diacylglycerol kinase zeta mouse (Dgkz Mouse)

Use

RNAi and Retroviral

Promoter

H1

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-Puro-BCL9

Plasmid

#202662

Purpose

Tet-ON 3rd generation expression vector with inducible expression of BCL9 and a puromycin selection cassette

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

BCL9 transcription coactivator (BCL9 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(RHOA(15))-PGKpuro2ABFP-W

Plasmid

#200493

Purpose

Lentiviral vector expressing gRNA targeting human RHOA

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

RHOA(15) (RHOA Human)

Use

Lentiviral

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(RHOA(12))-PGKpuro2ABFP-W

Plasmid

#200492

Purpose

Lentiviral vector expressing gRNA targeting human RHOA

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

RHOA(12) (RHOA Human)

Use

Lentiviral

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_GOLGA3_SLC35E3

Plasmid

#205826

Purpose

Express mEGFP-tagged fusion protein, GOLGA3_SLC35E3 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

GOLGA3_SLC35E3 (GOLGA3, SLC35E3 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_ABR_DUXA

Plasmid

#205767

Purpose

Express mEGFP-tagged fusion protein, ABR_DUXA from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

ABR_DUXA (ABR, DUXA Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF3238

Plasmid

#144714

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

GTF2A1 (GTF2A1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAG426GAL-FES

Plasmid

#203501

Purpose

Expresses human FES kinase from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

FES (FES Human)

Expression

Yeast

Promoter

GAL1

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_016-sgHRI

Plasmid

#202443

Purpose

Expression of a guide RNA targeting HRI

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

HRI gRNA (EIF2AK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF1840

Plasmid

#142866

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

GTF2A1 (GTF2A1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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