Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 10,377 results for: plasmids 101

Showing: 4381 - 4400 of 10377 results

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pREF-GFP kan

Plasmid

#122061

Purpose

reporter plasmid for termination and terminator override to express GFP. Marked with URA3

Depositor

Daniel Reines

Article

Reines et al Methods. 2019 Jan 4. pii: S1046-2023(18)

Inserts

intergenic terminator from yeast IMD2
green fluorescent protein

Expression

Yeast

Promoter

GAL1 and GAL1 comes in pYES2

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pET29b-mVenus-dspB(E184Q)-6xHis

Plasmid

#176576

Purpose

Plasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

Depositor

Mark Nitz

Article

Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

Insert

Dispersin B

Tags

Hexahistidine tag and mVenus

Expression

Bacterial

Mutation

E184Q

Promoter

T7

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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Syt1LeftArm-3XGS-GCaMP6s-SV40-3XP3-dsRed-SV40-Syt1RightArm

Plasmid

#159636

Purpose

Donor plasmid for generating Syt1:GCaMP6s strain in Aedes aegypti with CRISPR/Cas9

Depositor

Carolyn McBride

Article

Zhao et al Cell Rep Methods. 2021 Jul 26;1(3):10004

Insert

GCaMP6s

Expression

Insect

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pBN312

Plasmid

#86734

Purpose

GFP with Frt sites for FLP-mediated knock-out of gene function in C. elegans

Depositor

Peter Askjaer

Article

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

GFP_Frt_KO

Expression

Worm

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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PacrAB-acrAB-rfp + PmutS-mutS-yfp

Plasmid

#121443

Purpose

plasmid expressing translational fusions of AcrAB and MutS

Depositor

Mary Dunlop

Article

El Meouche et al Science. 2018 Nov 9;362(6415):686-690. d

Inserts

PmutS-mutS-yfp
PacrAB-acrAB-rfp

Use

Synthetic Biology; Pbb vector

Expression

Bacterial

Promoter

PacrAB and PmutS

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCJH026_proD_Cas12c-multiplexing_CAM

Plasmid

#183073

Purpose

Plasmid expressing a Cas12c sgRNA targeting GFP and another sgRNA targeting RFP in one transcript for testing multiplexed repression

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Expressing two Cas12c sgRNAs (targeting GFP or RFP) in one transcript

Use

CRISPR

Expression

Bacterial

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pET29b-mVenus-dspB(E184Q W330Y)-6xHis

Plasmid

#176577

Purpose

Plasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.

Depositor

Mark Nitz

Article

Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

Insert

Dispersin B

Tags

Hexahistidine tag and mVenus

Expression

Bacterial

Mutation

E184Q W330Y

Promoter

T7

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (EG.5)

Plasmid

#241114

Purpose

Expresses SARS-CoV-2 spike (EG.5) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (EG.5) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I, L24del, P25del, P26del, A27S, V83A, G142D, …

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_SARS2_XBB

Plasmid

#194494

Purpose

expressing SARS-CoV-2 XBB spike protein for pseudovirus production

Depositor

David Nemazee

Article

Zhou et al Immunity. 2023 Mar 14;56(3):669-686.e7.

Insert

Spike (XBB) (S SARS-CoV-2)

Expression

Mammalian

Mutation

V83A, Y144-,H146Q, Q183E, V213E, G339H, L368I, R3…

Promoter

CMV

Available Since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_SARS2_BQ.1.1

Plasmid

#194493

Purpose

expressing SARS-CoV-2 BQ.1.1 spike protein for pseudovirus production

Depositor

David Nemazee

Article

Zhou et al Immunity. 2023 Mar 14;56(3):669-686.e7.

Insert

Spike (BQ.1.1) (S SARS-CoV-2)

Expression

Mammalian

Mutation

R346T, K444T, N460K and c-terminal 18aa del on th…

Promoter

CMV

Available Since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pαH-S-GSAS-OMICRON.BA.2-RBD

Plasmid

#184830

Purpose

Mammalian cell expression of RBD of SARS-CoV-2 spike protein of variant OMICRON.BA.2 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987

Depositor

Priyamvada Acharya

Article

Stalls et al Cell Rep. 2022 Jun 28;39(13):111009. doi

Insert

S-GSAS-OMICRON.BA2-RBD (S Severe acute respiratory syndrome coronavirus 2)

Tags

6X His tag

Expression

Mammalian

Mutation

SARS-CoV-2 Spike RBD protein of OMICRON.BA.2 vari…

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pαH-S-GSAS-OMICRON.BA.1-RBD

Plasmid

#184831

Purpose

Mammalian cell expression of RBD of SARS-CoV-2 spike protein of variant OMICRON.BA.1 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987

Depositor

Priyamvada Acharya

Article

Stalls et al Cell Rep. 2022 Jun 28;39(13):111009. doi

Insert

S-GSAS-OMICRON.BA.1 (S Severe acute respiratory syndrome coronavirus 2)

Tags

6X His tag

Expression

Mammalian

Mutation

SARS-CoV-2 Spike RBD protein of OMICRON.BA.1 vari…

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (CH.1.1)

Plasmid

#241112

Purpose

Expresses SARS-CoV-2 spike (CH.1.1) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (CH.1.1) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I,L24del,P25del,P26del,A27S,G142D,K147E,W152R,…

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (XBB-3a)

Plasmid

#241123

Purpose

Expresses SARS-CoV-2 spike (XBB-3a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (XBB-3a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I,L24del,P25del,P26del,A27S,V83A,G142D,Y144del…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (XBB-4a)

Plasmid

#241124

Purpose

Expresses SARS-CoV-2 spike (XBB-4a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (XBB-4a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I,L24del,P25del,P26del,A27S,V83A,G142D,Y144del…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (XBB-2a)

Plasmid

#241122

Purpose

Expresses SARS-CoV-2 spike (XBB-2a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (XBB-2a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I,L24del,P25del,P26del,A27S,V83A,G142D,Y144del…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.5-5a)

Plasmid

#241107

Purpose

Expresses SARS-CoV-2 spike (BA.5-5a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.5-5a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I,L24del,P25del,P26del,A27L,H69del,V70del,T76I…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.5-5b)

Plasmid

#241108

Purpose

Expresses SARS-CoV-2 spike (BA.5-5b) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.5-5b) (S SARS-CoV-2)

Expression

Mammalian

Mutation

N481T,T76I,M153S,Y473F,Q493R,T19I,L24del,P25del,P…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.5-3b)

Plasmid

#241105

Purpose

Expresses SARS-CoV-2 spike (BA.5-3b) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.5-3b) (S SARS-CoV-2)

Expression

Mammalian

Mutation

Q493R,T76I,M153S,T19I,L24del,P25del,P26del,A27S,H…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.5-4a)

Plasmid

#241106

Purpose

Expresses SARS-CoV-2 spike (BA.5-4a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.5-4a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

Y473F,Q493R,T76I,M153S,T19I,L24del,P25del,P26del,…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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