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pBN449

Plasmid

#129555

Purpose

GAL4-induced expression of codon-optimised FLP recombinase with unfused mNeongreen (mNG) as a marker in C. elegans

Depositor

Peter Askjaer

Article

Ayuso et al MicroPubl Biol. 2019 Jan 29;2019:10.1791

Insert

15xUAS-∆pes-10-Flp-SL2

Tags

mNeonGreen

Expression

Worm

Promoter

UAS-∆pes-10

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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cmv 16 E7 del DLYC

Plasmid

#13687

Depositor

Karl Munger

Article

Gonzalez et al J Virol. 2001 Aug . 75(16):7583-91.

Insert

HPV 16 E7 del DLYC (E7 Human)

Expression

Mammalian

Mutation

del aa 21-24

Available Since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
P525

Plasmid

#139739

Purpose

Individual component of Caffeic Acid biosynthesis pathway, hpaB

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

hpaB

Expression

Plant

Promoter

35S

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pAE03

Plasmid

#61044

Purpose

To construct C-terminal GFP fusions, single crossover integration vector

Depositor

Jan-Willem Veening

Article

Eberhardt et al Mol Microbiol. 2009 Oct;74(2):395-408. d

Type

Empty backbone

Use

Synthetic Biology

Tags

GFP+

Expression

Bacterial

Available Since

Feb. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pCBLsgRNA1

Plasmid

#199727

Purpose

Golden Gate entry vector; 1st gRNA under OsU3 promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

OsU3-sgRNA1 scaffold

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCBLsgRNA2

Plasmid

#199728

Purpose

Golden Gate entry vector; 2nd gRNA under OsU3 promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

OsU3-sgRNA2 scaffold

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAB143

Plasmid

#101679

Purpose

LAA-tagged mCherry under T7 promoter control

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

LAA-tagged mCherry under T7 promoter control

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pRATIO2214

Plasmid

#141303

Purpose

Gateway cloning compatible dual fluorescent ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Tags

mCerulean and mScarlet-I

Expression

Plant

Promoter

UBQ10 promoter

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET15bLCFPT7

Plasmid

#53545

Purpose

Activation module. The plasmid consists of a mutant T7 RNA polymerase (T7 RNAP) activating its own expression via a T7 promoter carrying alac operator. Carbenicillin resistant

Depositor

Lingchong You

Article

Payne et al Mol Syst Biol. 2013 Oct 8;9:697. doi: 10

Insert

T7 RNAP

Expression

Bacterial

Available Since

July 28, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-Y-RAN-RFP6

Plasmid

#106424

Purpose

Y-RAN-RFP6 (RANbody RFP6::Chicken IgY)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

Y-RAN-RFP6

Tags

HA and His

Expression

Mammalian

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pMB62

Plasmid

#47944

Purpose

Expresses C. elegans optimized Cas9::mEGFP from the eef-1A.1 (eft-3) promoter

Depositor

Mike Boxem

Article

Waaijers et al Genetics. 2013 Aug 26.

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS, egl-13 NLS, and mEGFP

Expression

Worm

Promoter

eef-1A.1 (eft-3)

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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MAP72_MS2

Plasmid

#220623

Purpose

Cassette vector to enable overexpression of rNTases as MS2 coat protein fusions in S. cerevisiae.

Depositor

Marvin Wickens

Article

Preston et al Nat Methods. 2019 May;16(5):437-445. doi

Type

Empty backbone

Use

Shuttle vector

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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CrBlue-mcherry - pcDNA3.1

Plasmid

#64204

Purpose

CrBlue fused to mcherry on the C terminus

Depositor

Narasimhan Gautam

Article

Karunarathne et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

Insert

CrBlue

Tags

mcherry

Expression

Mammalian

Promoter

CMV

Available Since

April 13, 2015

Availability

Academic Institutions and Nonprofits only
P527

Plasmid

#139741

Purpose

Individual component of Caffeic Acid biosynthesis pathway, RcTAL

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

TAL

Expression

Plant

Promoter

35S

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV10-3xFLAG-prs-MCP1

Plasmid

#184341

Purpose

Express yeast MCP1 in Expi293F cells

Depositor

Karin Reinisch

Article

Adlakha et al J Cell Biol. 2022 Jun 6;221(5):e20220203

Insert

Yeast MCP1 (MCP1 Budding Yeast)

Tags

3xFLAG-PreScission cleavage site

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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XW52

Plasmid

#65838

Purpose

XW52 Pmig-13-Czipper-QF-AD-NLS-SL2-mCherry-3'UTR-unc-54 for Split Q intersectional method

Depositor

Kang Shen

Article

Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

Insert

Czipper-QF-AD-NLS

Tags

mCherry

Expression

Worm

Promoter

Pmig-13

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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P364

Plasmid

#139721

Purpose

Module plasmid, individual component of Fungal Bioluminescent Pathway, CPH

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pMOD_A'_pSlH4:NnCPH:tSlH4

Use

Luciferase

Expression

Plant

Promoter

SlH4

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pTK_Sox9_enh_349_Citrine

Plasmid

#130594

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Sox9_enh_349

Use

Enhancer reporter

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-RCC1-polyA

Plasmid

#59762

Purpose

Express Flag-tagged RCC1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

RCC1

Expression

Mammalian

Promoter

CMV and T7

Available Since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pCO604

Plasmid

#139737

Purpose

individual component of Fungal Bioluminescent Pathway, MoClo level 0 plasmid, NnH3H

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

H3H

Use

Luciferase; Moclo level 0 component

Promoter

none

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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