We narrowed to 11,977 results for: pcDNA

1. pCDNA3-AUP1-FLAG DTM

   Plasmid

   #185337

   Purpose

   Assessing domain requirements of AUP1

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   AUP1 (AUP1 Human)
   

   Use

   Tags

   FLAG, 6xHis

   Expression

   Mammalian

   Mutation

   deletion of aa 24-45, corresponding to the hairpi…

   Promoter

   Available since

   Feb. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3-AUP1-FLAG-DCUE

   Plasmid

   #185339

   Purpose

   Assessing domain requirements of AUP1

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   AUP1 (AUP1 Human)
   

   Use

   Tags

   FLAG, 6xHis

   Expression

   Mammalian

   Mutation

   deletion of aa 293-337 corresponding to the CUE d…

   Promoter

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-FLAG-MTOR-E1799K

   Plasmid

   #69009

   Purpose

   activating MTOR mutation

   Depositor

   David Sabatini

   Article

   Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

   Insert

   MTOR-E1799K (MTOR Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   change Glutamate 1799 to Lysine

   Promoter

   Available since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-Golgi-ExRai-AktAR2

   Plasmid

   #184051

   Purpose

   Golgi-targeted ExRai-AktAR2.

   Depositor

   Jin Zhang

   Article

   Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

   Insert

   Golgi-ExRai-AktAR2
   

   Use

   Tags

   N-terminal 35 amino acids from human endothelial …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-AKT-PH[R25C]-GFP

   Plasmid

   #18837

   Depositor

   Craig Montell

   Article

   Kwon et al Mol Cell. 2007 Feb 23. 25(4):491-503.

   Insert

   AKT-PH domain (AKT1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   R25C

   Promoter

   Available since

   July 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3 CHICA (Nigg HS342)

   Plasmid

   #41153

   Depositor

   Erich Nigg

   Article

   Santamaria et al Curr Biol. 2008 May 20;18(10):723-9.

   Insert

   CHICA (FAM83D Human)
   

   Use

   Tags

   3xMyc and PreScission Site

   Expression

   Mammalian

   Mutation

   V11L

   Promoter

   CMV

   Available since

   June 7, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA4-Beclin1 243-450

   Plasmid

   #24392

   Depositor

   Qing Zhong

   Article

   Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

   Insert

   Beclin1 (BECN1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains amino acids 243-450.

   Promoter

   Available since

   April 1, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3-Akt-STOPS-NES

   Plasmid

   #175008

   Purpose

   Cytosol-targeted Akt-STOPS.

   Depositor

   Jin Zhang

   Article

   Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

   Insert

   mCherry-Akt-STOPS-NES
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA4-Beclin1 151-241

   Plasmid

   #24390

   Depositor

   Qing Zhong

   Article

   Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

   Insert

   Beclin1 (BECN1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains amino acids 151-241.

   Promoter

   Available since

   April 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3_GLP1R-NTEV-TCS-GV-2xHA

    Plasmid

    #194378

    Purpose

    Split TEV assays

    Depositor

    Michael Wehr

    Article

    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

    Insert

    GLP1R-NTEV-TCS-GV-2xHA (GLP1R Human, Synthetic)
    

    Use

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3 PARL-FLAG-CT S277G

    Plasmid

    #13615

    Depositor

    Luca Pellegrini

    Article

    Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

    Insert

    Presenilin-associated rhomboid-like (PARL, PARL Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed catalytic Serine 277 to Alanine

    Promoter

    Available since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3-HA-eEF2K(S441A/S445A)

    Plasmid

    #110161

    Purpose

    Expression of human eEF2K (S441A/S445A) in mammalian cells

    Depositor

    Daniele Guardavaccaro

    Article

    Kruiswijk et al Sci Signal. 2012 Jun 5;5(227):ra40. doi:

    Insert

    eEF2K (eukaryotic elongation factor 2 kinase) (EEF2K Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S441A/S445A

    Promoter

    CMV

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-tRNA scaffolded SA (tRSA)

    Plasmid

    #32200

    Depositor

    Ian Macara

    Article

    Iioka et al Nucleic Acids Res. 2011 Apr;39(8):e53. E

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+)-HLA-cmyc-natT1R2

    Plasmid

    #113946

    Purpose

    mammalian expression plasmid for c-myc-tagged human T1R2 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R2 (TAS1R2 Human)
    

    Use

    Tags

    Signal/leader sequence from HLA class I histocomp…

    Expression

    Mammalian

    Mutation

    residues 22-839

    Promoter

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-mNeonGreen-LEHD-NanoLuc

    Plasmid

    #98289

    Purpose

    caspase-9 BRET Sensor

    Depositor

    Maarten Merkx

    Article

    den Hamer et al ACS Sens. 2017 Jun 23;2(6):729-734. doi:

    Insert

    mNeonGreen-LEHD-NanoLuc
    

    Use

    Tags

    His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5 FRT TO Myc ArA WT

    Plasmid

    #59804

    Purpose

    Allows the integration of Myc ArA in the genome and Tet-inducible expression

    Depositor

    Stephen Taylor

    Article

    Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

    Insert

    Aurora A (AURKA Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. hE-cadherin/α-catenin fusion-pcDNA3

    Plasmid

    #45771

    Depositor

    Barry Gumbiner

    Article

    Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

    Insert

    hE-cadherin/Xe α-catenin fusion (CDH1, ctnna1 Human, Frog)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Human E-cadherin (aa 1-801) fused to Xenopus α-ca…

    Promoter

    CMV

    Available since

    June 14, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3.1(-) rat C/EBP alpha

    Plasmid

    #12550

    Depositor

    Peter Johnson

    Article

    Miller et al J Biol Chem. 2003 Apr 25;278(17):15178-8

    Insert

    CCAAT/Enhancer-binding Protein alpha (Cebpa Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. GeNL(Ca2+)_60/pcDNA3

    Plasmid

    #85207

    Purpose

    luminescent reporter for calcium signaling

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    GeNL(Ca2+)_60
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA3 Flag Jnk2a2(apf)

    Plasmid

    #13761

    Depositor

    Roger Davis

    Article

    Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

    Insert

    Jnk2a2(apf) (MAPK9 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    replaced dual phosporylation motif THr(183)-Pro-T…

    Promoter

    Available since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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