We narrowed to 22,830 results for: Sis

1. pQC.Flip-nluc(LgBiT1-8)CP[TEV]

   Plasmid

   #200122

   Purpose

   Retroviral expression of Flip-nluc(LgBiT1-8)CP[TEV] protease reporter and T2A mCherry in mammalian cells

   Depositor

   Eiji Morita

   Article

   Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

   Insert

   Flip-nluc(LgBiT1-8)CP[TEV] T2A mCherry
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. OSCA1.1

   Plasmid

   #136592

   Purpose

   Expresses the mechanosensitive channel OSCA1.1 in mammalian cells

   Depositor

   Ardem Patapoutian

   Article

   Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10

   Insert

   OSCA1.1 (AT4G04340 Mustard Weed)
   

   Tags

   pIRES2-mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT2ADWpuro_2paRAF

   Plasmid

   #129653

   Purpose

   For generating cell line expressing the 2P-activatable RAF system with P2A and full-length RAF1

   Depositor

   Michiyuki Matsuda

   Article

   Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

   Insert

   2paCRY2-Raf1-2A-CIBN-mScarlet-KRasCAAX
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. p2T-CMV-eA3Amax-BlastR

   Plasmid

   #152997

   Purpose

   C-to-T base editor

   Depositor

   David Liu

   Article

   Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

   Insert

   eA3A-nSpCas9-UGI-UGI
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. FSW-HRP-V5-NLGN2

   Plasmid

   #82545

   Purpose

   Overexpression vector for V5-NLGN2 in neurons

   Depositor

   Alice Ting

   Article

   Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

   Insert

   NLGN2 (Nlgn2 Rat)
   

   Use

   Lentiviral

   Tags

   HRP and V5

   Expression

   Mammalian

   Promoter

   Human Synapsin

   Available Since

   Nov. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAC-GGPPipi

   Plasmid

   #53280

   Purpose

   Contains crtE and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces geranylgeranyl diphosphate in E. coli

   Depositor

   Francis X Cunningham Jr

   Article

   Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

   Insert

   crtE, idi
   

   Use

   Low copy number bacterial cloning vector

   Promoter

   endogenous promoters

   Available Since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCPP5741

   Plasmid

   #128722

   Purpose

   Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

   Depositor

   Alan Collmer

   Article

   Wei et al Cell Rep. 2018 May 8;23(6):1630-1638. do

   Insert

   shcV-hopV1
   

   Expression

   Bacterial

   Available Since

   Jan. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCPP5910

   Plasmid

   #128717

   Purpose

   Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

   Depositor

   Alan Collmer

   Article

   Wei et al Cell Rep. 2018 May 8;23(6):1630-1638. do

   Insert

   shcO-hopO1-1
   

   Expression

   Bacterial

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCPP5058

   Plasmid

   #128710

   Purpose

   Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

   Depositor

   Alan Collmer

   Article

   Wei et al Cell Rep. 2018 May 8;23(6):1630-1638. do

   Insert

   shcF-hopF2
   

   Expression

   Bacterial

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3 CDK7 D155A HA (P#812)

    Plasmid

    #14648

    Depositor

    Matija Peterlin

    Article

    Kanazawa et al Oncogene. 2003 Aug 28. 22(36):5707-11.

    Insert

    Cdk7 (CDK7 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D155A: dominant negative

    Available Since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pPB-cT3G-cERP2-TERF1

    Plasmid

    #222542

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of TERF1

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    TERF1 (TERF1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFYF1328 EGFP Site#3

    Plasmid

    #47513

    Purpose

    human gRNA expression vector targeting EGFP

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-EGFP site 3
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO.1-scrCdh20.1 GFP

    Plasmid

    #209106

    Purpose

    GFP expressing scramble of shRNA targeting Cdh20

    Depositor

    Cagla Eroglu

    Article

    Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

    Insert

    scrCdh20.1
    

    Available Since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Hip1R-tDimerRFP-N1

    Plasmid

    #27700

    Depositor

    Christien Merrifield

    Article

    Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

    Insert

    Hip1R (Hip1r Mouse)
    

    Tags

    tDimer-RFP

    Expression

    Mammalian

    Available Since

    May 4, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pBabe-puro-Smad4-Flag

    Plasmid

    #37041

    Depositor

    Sam Thiagalingam

    Article

    Papageorgis et al Cancer Res. 2011 Feb 1;71(3):998-1008. E

    Insert

    Smad4 (SMAD4 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    5'LTR

    Available Since

    March 1, 2013

    Availability

    Academic Institutions and Nonprofits only

16. mEos4b-Lifeact-7

    Plasmid

    #54813

    Purpose

    Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Loren Looger, Michael Davidson

    Article

    Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Type

    Empty backbone

    Tags

    mEos4b-Lifeact

    Expression

    Mammalian

    Available Since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. FSW-HRP-V5-SLITRK3

    Plasmid

    #82539

    Purpose

    Lentiviral vector for expressing HRP-SLITRK3 in neurons

    Depositor

    Alice Ting

    Article

    Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

    Insert

    SLITRK3 (SLITRK3 Human)
    

    Use

    Lentiviral

    Tags

    HRP and V5

    Expression

    Mammalian

    Promoter

    Human Synapsin

    Available Since

    Sept. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMaCTag-P22

    Plasmid

    #120033

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with SNAP

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    SNAP
    

    Use

    Pcr template

    Tags

    SNAP

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pWaldo-GFPe-SH1446

    Plasmid

    #112507

    Purpose

    Expresses SH1446 in E. coli with a C-terminal TEV-GFP-His(8) tag.

    Depositor

    Simon Newstead

    Article

    Minhas et al Elife. 2018 Jul 3;7. pii: 34995. doi: 10

    Insert

    POT family transporter
    

    Tags

    C terminal TEV GFP octa-His affinity tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLv-CMV-MyoD

    Plasmid

    #26808

    Depositor

    Jeffrey Chamberlain

    Article

    Kimura et al Hum Mol Genet. 2008 Aug 15. 17(16):2507-

    Insert

    MyoD (Myod1 Mouse)
    

    Use

    Lentiviral

    Available Since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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21. pYPQ210

    Plasmid

    #109329

    Purpose

    Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and bar gene for plant selection

    Depositor

    Yiping Qi

    Article

    Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

    Type

    Empty backbone

    Use

    Gateway compatible attr1-attr2 destination vector

    Expression

    Plant

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. hRIP1 HA GFP K45A

    Plasmid

    #41389

    Depositor

    Francis Chan

    Article

    Cho et al Cell. 2009 Jun 12;137(6):1112-23.

    Insert

    RIP1 (RIPK1 Human)
    

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    Kinase Domain Mutant (K45A)

    Promoter

    CMV

    Available Since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

23. pET151-hUCH37 (ISF1)

    Plasmid

    #61929

    Purpose

    Expression of hUCH37 (ISF1) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Insert

    hUCH37 (ISF1) (UCHL5 Human)
    

    Tags

    6x HIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

24. Psicheck2 PTPRN2 full length 3'UTR

    Plasmid

    #26994

    Depositor

    Joan Massague

    Article

    Tavazoie et al Nature. 2008 Jan 10. 451(7175):147-52.

    Insert

    PTPRN2 Full length 3' UTR (PTPRN2 Human)
    

    Use

    Luciferase

    Tags

    Renilla Luciferase

    Expression

    Mammalian

    Available Since

    Jan. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

25. pcDNA5/FRT/TO HSPA6

    Plasmid

    #19459

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    HSPA6 (HSPA6 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. OSCA1.8

    Plasmid

    #136594

    Purpose

    Expresses the mechanosensitive channel OSCA1.8 in mammalian cells

    Depositor

    Ardem Patapoutian

    Article

    Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10

    Insert

    OSCA1.8 (AT1G32090 Mustard Weed)
    

    Tags

    pIRES2-mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. EYFP-Rab7

    Plasmid

    #20164

    Depositor

    Xiaowei Zhuang

    Article

    Lakadamyali et al Cell. 2006 Mar 10. 124(5):997-1009.

    Insert

    Rab7 (RAB7A Canis lupus familiaris)
    

    Expression

    Mammalian

    Available Since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

28. pWPI-TFDP1

    Plasmid

    #114299

    Purpose

    Constitutive expression of the TFDP1 protein

    Depositor

    Patrick Salmon

    Article

    Rady et al Cell Cycle. 2013 Aug 15;12(16):2691-702.

    Insert

    TFDP1 (TFDP1 Human)
    

    Use

    Lentiviral

    Available Since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. 35S-DsREDmonomer-NosT

    Plasmid

    #79183

    Purpose

    Transient expression vector for DsRED monomer

    Depositor

    Yutaka Kodama, Masamitsu Wada

    Article

    Kodama et al Proc Natl Acad Sci U S A. 2010 Nov 9;107

    Type

    Empty backbone

    Use

    Reporter plasmid

    Tags

    DsREDmonomer

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMT-Aurora B-GFP

    Plasmid

    #24285

    Depositor

    Ron Vale

    Article

    Vale et al J Cell Biol. 2009 Sep 7. 186(5):727-38.

    Insert

    Aurora B Kinase (aurB Fly)
    

    Tags

    GFP

    Expression

    Insect

    Available Since

    May 10, 2010

    Availability

    Academic Institutions and Nonprofits only

31. pET-mF(3230)A3

    Plasmid

    #168016

    Purpose

    Expresses MBP-F(3230)A3-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    MBP-F(3230)A3-6xHis
    

    Tags

    Hisx6 and maltose binding protein

    Expression

    Bacterial

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAAV-rTH-tTA

    Plasmid

    #133268

    Purpose

    An AAV genome that expresses tTA from the rat TH promoter

    Depositor

    Viviana Gradinaru

    Article

    Robinson et al Elife. 2019 Sep 23;8. pii: 48983. doi: 1

    Insert

    tTA
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    *(see below)

    Promoter

    rTH

    Available Since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCAG-mWasabi-G

    Plasmid

    #164161

    Purpose

    Expresses mWasabi under the control of CAG promoter, basic vector, multiclonig site at C terminal

    Depositor

    Isei Tanida

    Article

    Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

    Insert

    mWasabi
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSV-PGC1

    Plasmid

    #3

    Depositor

    Bruce Spiegelman

    Article

    Puigserver et al Cell 1998 Mar 20;92(6):829-39.

    Insert

    PGC-1 alpha (Ppargc1a Mouse)
    

    Expression

    Mammalian

    Available Since

    May 23, 2006

    Availability

    Academic Institutions and Nonprofits only

35. pMS84 (U6p::GGACAGTCCTGCCGAGGTGG)

    Plasmid

    #193852

    Purpose

    Encodes the guide RNA targeting the sequence GGACAGTCCTGCCGAGGTGG

    Depositor

    Patrick Phillips

    Article

    Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

    Insert

    Guide RNA
    

    Use

    CRISPR

    Expression

    Worm

    Promoter

    U6

    Available Since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLV-EF1a-NucGFP

    Plasmid

    #184706

    Purpose

    Lentiviral transfer plasmid for expression of NucGFP

    Depositor

    Brian Van Tine

    Article

    Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

    Insert

    NucGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Fragment 1 Null

    Plasmid

    #198729

    Purpose

    Gateway-compatible gRNA backbone . Template for the addition of target sequences to produce 2x gRNA.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    gRNA expression cassette in pENTR4
    

    Use

    Gateway-compatible entry vector

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. HBT_pcoCASphi_version1

    Plasmid

    #197931

    Purpose

    Cloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the N-terminal and nucleoplasmin NLS at the C-terminal

    Depositor

    Steven Jacobsen

    Article

    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

    Insert

    pcoCasphi-2
    

    Use

    CRISPR

    Tags

    FLAG tag and SV40 NLS and nucleoplasmin NLS

    Expression

    Plant

    Promoter

    35SPPDK promoter

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMaCTag-P26

    Plasmid

    #120037

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mAID-Clover

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mAID-Clover
    

    Use

    Pcr template

    Tags

    mAID-Clover

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCas3

    Plasmid

    #229538

    Purpose

    pMV_hyg encoding Cas3(wt) and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning.

    Depositor

    Kevin Verstrepen

    Article

    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

    Insert

    Cas3
    

    Use

    CRISPR

    Expression

    Bacterial and Yeast

    Mutation

    codon optimized for S.cerevisiae

    Available Since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge