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pcDNA3-Y478C-WLS-HA-IRES-GFP

Plasmid

#178063

Purpose

Expression of WLS-HA protein (Y478C mutant)

Depositor

Joseph Gleeson

Article

Chai et al N Engl J Med. 2021 Sep 30;385(14):1292-1

Insert

WLS (WLS Human)

Tags

HA and IRES-GFP

Expression

Mammalian

Mutation

Y478C

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Y392C-WLS-HA-IRES-GFP

Plasmid

#178062

Purpose

Expression of WLS-HA protein (Y392C mutant)

Depositor

Joseph Gleeson

Article

Chai et al N Engl J Med. 2021 Sep 30;385(14):1292-1

Insert

WLS (WLS Human)

Tags

HA and IRES-GFP

Expression

Mammalian

Mutation

Y392C

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-I531T-WLS-HA-IRES-GFP

Plasmid

#178064

Purpose

Expression of WLS-HA protein (I531T mutant)

Depositor

Joseph Gleeson

Article

Chai et al N Engl J Med. 2021 Sep 30;385(14):1292-1

Insert

WLS (WLS Human)

Tags

HA and IRES-GFP

Expression

Mammalian

Mutation

I531T

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HOXD8

Plasmid

#101402

Purpose

Donor Vector containing HOXD8 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

HOXD8 (HOXD8 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-ALK-R401Q

Plasmid

#116112

Purpose

Lentiviral expression of ALK R401Q

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ALK (ALK Human)

Use

Lentiviral

Mutation

R401Q

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-ALK-C990R

Plasmid

#116107

Purpose

Lentiviral expression of ALK C990R

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ALK (ALK Human)

Use

Lentiviral

Mutation

C990R

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_HAPLN1_p.S326N

Plasmid

#81640

Purpose

Gateway Donor vector containing HAPLN1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HAPLN1 (HAPLN1 Human)

Use

Gateway entry vector

Mutation

S326N

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_HAPLN1_p.K115N

Plasmid

#81646

Purpose

Gateway Donor vector containing HAPLN1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HAPLN1 (HAPLN1 Human)

Use

Gateway entry vector

Mutation

K115N

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_HAPLN1_p.P233H

Plasmid

#81648

Purpose

Gateway Donor vector containing HAPLN1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HAPLN1 (HAPLN1 Human)

Use

Gateway entry vector

Mutation

P233H

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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CSF1R gRNA (BRDN0001146637)

Plasmid

#77038

Purpose

3rd generation lentiviral gRNA plasmid targeting human CSF1R

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CSF1R (CSF1R Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_EML4_ALK

Plasmid

#205809

Purpose

Express mEGFP-tagged fusion protein, EML4_ALK from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

EML4_ALK (EML4, ALK Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223-CSNK2A1

Plasmid

#23678

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CSNK2A1 (CSNK2A1 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pZB246-T7-T+

Plasmid

#250903

Purpose

Expresses N-terminal His-tag thermally stabilized T7 RNAP under a lac-inducible promoter on Kanamycin Resistance Backbone

Depositor

Timothy Whitehead

Article

Baumer et al bioRxiv [Preprint]. 2025 Nov 12:2025.11.

Insert

Thermally Stable T7 RNA Polymerase

Tags

6xHis

Expression

Bacterial

Mutation

Arginine 31 to Aspartic Acid, Alanine 61 to Argin…

Promoter

pT5-lacUV

Available Since

Feb. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-Sport6-CD63-pHluorin

Plasmid

#130901

Purpose

Expression of CD63-pHluorin for visualization of multivesicular body-plasma membrane fusion.

Depositor

DM Pegtel

Article

Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

Insert

CD63-pHluorin (CD63 Aequorea victoria, Human)

Tags

pHluorin

Expression

Mammalian

Mutation

pHluorin inserted between Gln-36 and Leu-37 in ex…

Promoter

CMV

Available Since

Oct. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-Sport6-CD63-pHuji

Plasmid

#130902

Purpose

Expression of CD63-pHuji for visualization of multivesicular body-plasma membrane fusion.

Depositor

DM Pegtel

Article

Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

Insert

CD63-pHuji (CD63 Human, synthetic)

Tags

pHuji

Expression

Mammalian

Mutation

pHuji inserted between Gln-36 and Leu-37 in extra…

Promoter

CMV

Available Since

Oct. 10, 2019

Availability

Academic Institutions and Nonprofits only

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AAVS1 CAG rtTA3 TauP301L 2N4R-EGFP

Plasmid

#132393

Purpose

Exchange cassette containing inducible TauP301L 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site

Depositor

Gerold Schmitt-Ulms

Article

Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

Insert

human TauP301L 2N4R-EGFP (MAPT Synthetic, Human)

Use

CRISPR and Cre/Lox

Tags

EGFP

Expression

Mammalian

Mutation

Proline 301 to Leucine (P301L)

Promoter

TREtight

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5-EGFP-Tau E14

Plasmid

#46907

Purpose

expresses EGFP tagged Tau E14 in mammalian cells

Depositor

Karen Ashe

Article

Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

Insert

Tau E14 (MAPT Human)

Tags

EGFP

Expression

Mammalian

Mutation

all 14 S/P or T/P amino acid residues (T111, T153…

Promoter

CMV

Available Since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

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AAVS1 CAG rtTA3 TauWT 2N4R-EGFP

Plasmid

#132389

Purpose

Exchange cassette containing inducible TauWT 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site

Depositor

Gerold Schmitt-Ulms

Article

Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

Insert

human TauWT 2N4R-EGFP (MAPT Synthetic, Human)

Use

CRISPR and Cre/Lox

Tags

EGFP

Expression

Mammalian

Promoter

TREtight

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-Sport6-CD81-pHluorin

Plasmid

#130903

Purpose

Expression of CD81-pHluorin for visualization of multivesicular body-plasma membrane fusion.

Depositor

DM Pegtel

Article

Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

Insert

CD81-pHluorin (CD81 Human, aequorea victoria)

Tags

pHluorin

Expression

Mammalian

Mutation

pHluorin inserted between Leu-49 and Gly-50 in ex…

Promoter

CMV

Available Since

Oct. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-EPCAM-Fc(DAPA)-AviTag-6xHis

Plasmid

#156693

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

EPCAM (EPCAM Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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