We narrowed to 19,813 results for: KIN

1. DYNLT3 B3.5 gRNA

   Plasmid

   #90670

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human DYNLT3

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   DYNLT3 (Guide Designation B3.5)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   May 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. DYNLRB1 H11.3 gRNA

   Plasmid

   #90664

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human DYNLRB1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   DYNLRB1 (Guide Designation H11.3)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   May 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pVR2

   Plasmid

   #84719

   Purpose

   E. coli RecA promoter followed by stretch with no A's followed by riboswitch for single-round transcription

   Depositor

   Nils Walter

   Article

   Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d

   Inserts

   E. coli RecA promoter
   stretch lacking A's followed by 5' UTR of queC gene
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   12 nt stretch lacking A's inserted before 5&…

   Promoter

   Available since

   March 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PAK4-3'UTR-BS2

   Plasmid

   #113091

   Purpose

   Luciferase reporter containing PAK4 3' UTR with the second predicted MiR-199a-3p binding

   Depositor

   James Donahue

   Article

   Phatak et al Oncotarget. 2018 Jun 19;9(47):28391-2840

   Insert

   p21 Activated kinase 4 (PAK4 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   PGK

   Available since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. TGIF2 A11.3 gRNA

   Plasmid

   #90910

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TGIF2

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   TGIF2 (Guide Designation A11.3)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a_6HT-TBP6.7(WT)

   Plasmid

   #112720

   Purpose

   Wild-type TBP6.7 was one of the first evolved TAR-binding proteins to bind with exceptional affinity. It was this protein that was used to obtain co-crystals with WT TAR RNA.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(WT)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a_6HT-TBP6.7(P46A)

   Plasmid

   #112721

   Purpose

   This mutation (P46A) marks the first residue within the evolved region of U1A.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(P46A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   P46A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a_6HT-TBP6.7(Q48T)

   Plasmid

   #112725

   Purpose

   This mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(Q48T)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   Q48T

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a_6HT-TBP6.7(P51A)

   Plasmid

   #112728

   Purpose

   Similar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(P51A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   P51A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a_6HT-TBP6.7(Q54A)

    Plasmid

    #112730

    Purpose

    Although not evolved, this residue within TBPs participates in stabilizing the polypeptide loop responsible for RNA recognition.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(Q54A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    Q54A

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PAK4-3'UTR-BS1

    Plasmid

    #113090

    Purpose

    Luciferase reporter containing PAK4 3' UTR with the first predicted MiR-199a-3p binding site

    Depositor

    James Donahue

    Article

    Phatak et al Oncotarget. 2018 Jun 19;9(47):28391-2840

    Insert

    p21 Activated kinase 4 (PAK4 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    PGK

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TTK H10.1 gRNA

    Plasmid

    #90923

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TTK

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    TTK (Guide Designation H10.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. NDE1 F1.2 gRNA

    Plasmid

    #90788

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human NDE1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    NDE1 (Guide Designation F1.2)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AURKA A1.1 gRNA

    Plasmid

    #90540

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human AURKA

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    AURKA (Guide Designation A1.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CKS2 C8.2 gRNA

    Plasmid

    #90633

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CKS2

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    CKS2 (Guide Designation C8.2)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CCNA1 C8.5 gRNA

    Plasmid

    #90573

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CCNA1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    CCNA1 (Guide Designation C8.5)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. H2AFZ B2.3 gRNA

    Plasmid

    #90705

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human H2AFZ

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    H2AFZ (Guide Designation B2.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. KIF4A D10.2 gRNA

    Plasmid

    #90725

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human KIF4A

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    KIF4A (Guide Designation D10.2)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. UNG D11.3 gRNA

    Plasmid

    #90936

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human UNG

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    UNG (Guide Designation D11.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MCM6 E2.2 gRNA

    Plasmid

    #90763

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MCM6

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    MCM6 (Guide Designation E2.2)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge