We narrowed to 29,515 results for: REP

1. EZH2-TALEN_Right_No_FLAG

   Plasmid

   #79900

   Purpose

   Untagged EZH2-TALEN_Right to target the N-terminus of human EZH2

   Depositor

   Yannick Doyon

   Article

   Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

   Insert

   EZH2-TALEN_Right_No_FLAG (EZH2 Human)
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBB0323-TEV-His12

   Plasmid

   #137039

   Purpose

   expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0323 with C-terminal TEV-protease-cleavable His12

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Insert

   AAC66700.1
   

   Use

   Tags

   His12 (TEV protease cleavable)

   Expression

   Bacterial

   Mutation

   wild type, full-length protein, contains signal p…

   Promoter

   T7

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. vsv-CenpB 1-166-Sgo1 N61I-GFP

   Plasmid

   #108499

   Purpose

   expression of vsv-CenpB 1-166-Sgo1 N61I-EGFP (no PP2A binding)

   Depositor

   Susanne Lens

   Article

   Meppelink et al Cell Rep. 2015 Apr 28;11(4):508-15. doi:

   Insert

   CenpB-Shugoshin 1 (SGO1 Human)
   

   Use

   Tags

   EGFP and VSV

   Expression

   Mammalian

   Mutation

   CenpB 1-166 fusion before Sgo1, no PP2A binding N…

   Promoter

   CMV

   Available since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-PH-SWAP70(R223E, R224E)

   Plasmid

   #84583

   Purpose

   Expresses GFP-tagged PH-domain of SWAP70 in mammalian cells, impaired phosphoinositide binding

   Depositor

   Geert van den Bogaart

   Article

   Baranov et al Cell Rep. 2016 Nov 1;17(6):1518-1531. do

   Insert

   SWAP70 residues 209-306 with mutations R223E and R224E (SWAP70 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Pleckstrin-homology domain, residues 209-306, mut…

   Promoter

   CMV

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

5. p3E DN Kif5a

   Plasmid

   #105973

   Purpose

   3' element for Gateway cloning, 5 single nucleotide mutations compared to the reference sequence, but none of them change the amino acidtruncation aa416-1033(end) 1854 bp

   Depositor

   Caren Norden

   Article

   Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

   Insert

   Kif5a (kif5aa Zebrafish)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   truncation of aa 416-1033

   Promoter

   Available since

   Feb. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKLV2-U6gRNA5(Vps39-g1)-PGKpuroBFP-W

   Plasmid

   #105019

   Purpose

   Lentiviral gRNA plasmid targeting mouse Vps39 , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Vps39 (Vps39 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-FLEX-SaCas9-U6-sgGabrg3

   Plasmid

   #124858

   Purpose

   Mutagenesis of Gabrg3

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Gabrg3 (Gabrg3 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-FLEXfrt-SaCas9-U6-sgGrin2a

   Plasmid

   #124863

   Purpose

   Mutagenesis of Grin2a

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Grin2a (Grin2a Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-FLEXfrt-SaCas9-U6-sgGabrg3

   Plasmid

   #124872

   Purpose

   Mutagenesis of Gabrg3

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Gabrg3 (Gabrg3 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-FLEXfrt-SaCas9-U6-sgGabrg2

    Plasmid

    #124871

    Purpose

    Mutagenesis of Gabrg2

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gabrg2 (Gabrg2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDEST-EGFP-Cx43ML-N1

    Plasmid

    #49860

    Purpose

    Encodes human connexin 43 with all internal methionines mutated to leucine and a C_terminal EGFP fusion tag

    Depositor

    Robin Shaw

    Article

    Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

    Insert

    Connexin 43 (GJA1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    All internal Methionines mutated to Leucine. No s…

    Promoter

    CMV

    Available since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA5-FRT-TO-vsv-Incenp wt-GFP

    Plasmid

    #108502

    Purpose

    inducible expression of vsv-INCENP wt-EGFP

    Depositor

    Susanne Lens

    Article

    van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

    Insert

    INCENP (INCENP Human)
    

    Use

    Tags

    EGFP and VSV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAWp40

    Plasmid

    #157980

    Purpose

    pFUGW-EFSp-BFP-{SbfI}

    Depositor

    Alan Siu Lun Wong

    Article

    Zhou et al Cell Rep. 2020 Aug 11;32(6):108020. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGGS_m-AKAR5’

    Plasmid

    #114140

    Purpose

    Mammalian expression of the AKAR5’ sensor C-terminally fused to the C-terminus of KRas (membrane targeting) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM

    Depositor

    Haining Zhong

    Article

    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

    Insert

    AKAR5' C-terminally tagged by KRas C tail
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5FRT/TO-UBE2QL1-GFP

    Plasmid

    #124667

    Purpose

    Expresses UBE2QL1 with C-terminal GFP tag in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

    Insert

    UBE2QL1 (UBE2QL1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. R725-X09-635: His6-tev-Hs.SPRED1(13-125)

    Plasmid

    #159581

    Purpose

    Baculovirus protein expression of His6-tev-Hs.SPRED1(13-125)

    Depositor

    Dominic Esposito

    Article

    Yan et al Cell Rep. 2020 Jul 21;32(3):107909. doi:

    Insert

    His6-tev-Hs.SPRED1(13-125) (SPRED1 Human)
    

    Use

    Tags

    His6-tev

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSN67

    Plasmid

    #100124

    Purpose

    expresses putative KLF4 DBD with a 6xHis tag

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    mCherry-P2A-putative KLF4 DBD with a 6xHis tag (KLF4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Insert corresponds to the DBD of KLF4

    Promoter

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSLQ1371-sgITGB1-3

    Plasmid

    #121534

    Purpose

    sgITGB1-3 sequence: GTTCAGTGAATGGGAACAACG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

    Depositor

    Ovijit Chaudhuri, Stanley Qi

    Article

    Lee et al Sci Rep. 2019 Nov 20;9(1):17188. doi: 10

    Insert

    sgITGB1-3
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    Nov. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. NG1301 pCI-TPI-4MS2-SMG5(short)-4H

    Plasmid

    #65806

    Purpose

    NMD reporter mRNA

    Depositor

    Niels Gehring

    Article

    Boehm et al Cell Rep. 2014 Oct 23;9(2):555-68. doi:

    Insert

    TPI (TPI1 Human)
    

    Use

    Tags

    4 MS2 stem loops, SMG5 3'UTR shortened versi…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.2-Cx43-NoStart

    Plasmid

    #49853

    Purpose

    Encodes human connexin 43 with the start methionine codon mutated to isoleucine and with wobble mutations conferring resistance against an siRNA

    Depositor

    Robin Shaw

    Article

    Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

    Insert

    Connexin 43 (GJA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Start codon mutated to from methionine to isoleuc…

    Promoter

    CMV

    Available since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only