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pZR040_Lenti-U6-sgCtrl-MS2-PP7-hPGK-PCP-p65-HSF1-Puro

Plasmid

#180266

Purpose

Lentiviral expression vector for CRISPRa-SAM with non-targeting control sgRNA

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

No-Target Control sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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LZRS-hNEDD9_IresGFP

Plasmid

#21962

Depositor

Lynda Chin

Article

Kim et al Cell. 2006 Jun 30. 125(7):1269-81.

Insert

NEDD9 (NEDD9 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

March 3, 2010

Availability

Academic Institutions and Nonprofits only
pPB-cT3G-cERP2-RUNX2

Plasmid

#192926

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of RUNX2

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

RUNX2 (RUNX2 Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pET246-pUC57 12xMS2V6

Plasmid

#104390

Purpose

MS2V6 cloning

Depositor

Robert Singer, Evelina Tutucci

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

12xMS2V6

Expression

Bacterial

Mutation

All Loops are U-variants, interspaced by 50 nucle…

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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Bxb1_tdTomato

Plasmid

#198041

Purpose

Bxb1-specific donor plasmid for integrating a transcription unit for constitutive expression of tdTomato reporter.

Depositor

Richard Davis

Article

Blanch-Asensio et al Nat Protoc. 2024 Aug 23. doi: 10.1038/s4

Insert

tdTomato

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-Kir2.1-T2A-tdTomato

Plasmid

#60598

Purpose

Expresses Kir2.1-T2A-tdTomato

Depositor

Massimo Scanziani

Article

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

Kir2.1-T2A-tdTomato (Kcnj2 Mouse)

Tags

myc

Expression

Mammalian

Available Since

Nov. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pB-CuR-hSOD1

Plasmid

#232477

Purpose

The inducible PiggyBac Cumate Switch vector (PBQM812A-1 System Biosciences) expressing human SOD1gene including flag -tag (DYKDDDDK) on the 3' end

Depositor

Lukas Trantirek

Article

Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1

Insert

Homo sapiens superoxide dismutase 1 (SOD1 Human)

Use

Transposon system

Tags

DYKDDDDK (FLAG-tag)

Expression

Mammalian

Promoter

CMV-CuO

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pTXB1-nbMmIgG2a-Tn5

Plasmid

#184288

Purpose

Anti-mouse IgG2a Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)

Depositor

New York Genome Center, Ivan Raimondi

Article

Stuart et al Nat Biotechnol. 2022 Dec 19. doi: 10.103

Insert

nbMmIgG2a-Tn5

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCB168

Plasmid

#37389

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

Insert

dynein cytoplasmic 1 intermediate chain 1 (DYNC1I1 Human)

Use

Retroviral

Tags

GFP, S-tag, and TEV

Expression

Mammalian

Available Since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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pAS168

Plasmid

#194491

Purpose

Zif268 Op (Part 2)

Depositor

Ahmad Khalil

Article

Sanford et al ACS Synth Biol. 2022 Nov 11. doi: 10.102

Insert

Zif268 Op (Part 2)

Use

Synthetic Biology

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pACYC-GroEL/ES-TF

Plasmid

#83923

Purpose

Co-expresses cytoplasmic copies of the Gro EL/ES and Trigger Factor protein folding chaperones.

Depositor

Karl Griswold

Article

Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

Inserts

Gro EL/ES
Trigger Factor

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pDY2261 hACTB atgRNA Paired Guide 1

Plasmid

#220991

Purpose

hACTB atgRNA

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

Insert

ACTB atgRNA (ACTB Human)

Use

CRISPR

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pDY2262 hACTB atgRNA Paired Guide 2

Plasmid

#220992

Purpose

hACTB atgRNA

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

Insert

ACTB atgRNA (ACTB Human)

Use

CRISPR

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO NL-FKBP-Cas9

Plasmid

#162730

Purpose

Bacterial expression, protein expression

Depositor

Amit Choudhary

Article

Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

Insert

FKBP F36V

Tags

6xHis and SUMO

Expression

Bacterial

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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Tailless VinculinTS

Plasmid

#26020

Depositor

Martin Schwartz

Article

Grashoff et al Nature. 2010 Jul 8. 466(7303):263-6.

Insert

tailless vinculinTS

Expression

Mammalian

Available Since

Sept. 17, 2010

Availability

Academic Institutions and Nonprofits only

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L444P GBA pcDNA3.1

Plasmid

#188582

Purpose

Ectopic expression L444P GBA

Depositor

Anthony Schapira

Article

Smith et al Hum Mol Genet. 2022 Sep 20:ddac233. doi:

Insert

GBA (GBA1 Human)

Expression

Mammalian

Mutation

L444P

Promoter

CMV

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pJRH-1363 LV EF1a-CD19 IRES-EGFP

Plasmid

#201919

Purpose

2nd generation lentiviral transfer plasmid for engineering cells to express human CD19

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

CD19 (CD19 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-XL4 ASXL1 (1-1304)

Plasmid

#74244

Purpose

Cancer-associated ASXL1 truncation mutant in mammalian expression vector

Depositor

Anjana Rao

Article

Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

Insert

ASXL1 (ASXL1 Human)

Expression

Mammalian

Mutation

Contains ASXL1 amino acids 1-1304

Promoter

CMV

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pET30a-GABAT

Plasmid

#239572

Purpose

Expresses E. coli GABA transaminase (GABA-T/gabT) for recombinant protein purification. Contains a His6-tag on its C-terminus for Ni2+ affinity chromatography.

Depositor

William Plaxton

Article

Benidickson et al Biol Methods Protoc. 2025 Jun 20;10(1):b

Insert

4-aminobutyrate aminotransferase (gabT E. coli)

Tags

His6

Expression

Bacterial

Available Since

July 18, 2025

Availability

Academic Institutions and Nonprofits only

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TBXTA-c026

Plasmid

#139763

Purpose

Protein expression/GST pull-down

Depositor

Opher Gileadi

Insert

TBXT, Full-length, WT (TBXT Human)

Tags

His6-GST-TEV

Expression

Bacterial

Mutation

Contains only amino acids S2- M435

Promoter

T7

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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