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Showing: 4441 - 4460 of 8708 results

  2. pUC_sgRNA

    Plasmid
    #68710
    Purpose
    Contains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.
    Depositor
    Roberto Docampo
    Article
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    68710-plasmid-map-sequence-id-123992

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  12. pFA NuiHA-loxP-nat-CUP1p

    Plasmid
    #131170
    Purpose
    PCR template for genomic N-terminal Nui-HA tag, nat-CUP1p flanking loxP sites allow expression by the native promoter after CRE-recombinase expression, nat marker confers resistance to Nourseothricin
    Depositor
    Nils Johnsson
    Article
    Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:
    Type
    Empty backbone
    Use
    Tags
    Nui-HA
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Sept. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    131170-plasmid-map-sequence-id-255831

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  14. pAG25-L-IFP-F[1] (clon 3)

    Plasmid
    #52899
    Purpose
    Plasmid template for PCR of cassettes for HR, used to introduce IFP F[1] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.
    Depositor
    Stephen Michnick
    Article
    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:
    Insert
    L-IFP-F[1]
    Use
    Vector for homologous recombination
    Tags
    Expression
    Mutation
    Promoter
    TEF promoter
    Available since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only

  15. pAG32-L-IFP-F[2] (clon 3)

    Plasmid
    #52900
    Purpose
    Plasmid template for PCR of cassettes for HR, used to introduce IFP F[2] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.
    Depositor
    Stephen Michnick
    Article
    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:
    Insert
    L-IFP-F[2]
    Use
    Vector for homologous recombination
    Tags
    Expression
    Mutation
    Promoter
    TEF promoter
    Available since
    June 5, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. pMOTag-M9-4H

    Plasmid
    #106379
    Purpose
    Can be used as a PCR template to amplify the M9-HA point mutant of glmS fragment for gene specific tagging that only allows tagging but not regulation. Acts as a control for glmS based gene regulation
    Depositor
    Roberto Docampo
    Article
    Cruz-Bustos et al J Eukaryot Microbiol. 2017 Dec 19. doi:
    Insert
    GlmS with M9 mutation
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 2, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    106379-plasmid-map-sequence-id-204790

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Showing: 4441 - 4460 of 8708 results