We narrowed to 17,703 results for: SHE

1. pTre-Tight-luciferase-Hottip (522)

   Plasmid

   #65490

   Purpose

   positive control for lncRNA expression assay

   Depositor

   Roderic Guigo, Rory Johnson

   Insert

   Hottip (HOTTIP Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   tight TRE promoter

   Available Since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpot-Tag-Lamin

   Plasmid

   #117062

   Purpose

   For mammalian expression of lamin B1 with N-terminal Spot-Tag

   Depositor

   Felix Hartlepp

   Insert

   Lamin B1 (LMNB1 Human)
   

   Tags

   Spot-Tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCLEN

   Plasmid

   #17704

   Depositor

   Nicholas Gaiano

   Article

   Gaiano et al Neuron. 2000 May . 26(2):395-404.

   Insert

   NIC (Notch1 Mouse)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   May 12, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pENTRY4_HuD

   Plasmid

   #65754

   Purpose

   entry vector with HuD

   Depositor

   Thomas Tuschl

   Insert

   ELAVL4 (ELAVL4 Human)
   

   Expression

   Mammalian

   Available Since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. p-dCas9-p300-S1396R-S1397R-Hygro

   Plasmid

   #104419

   Purpose

   transient expression of dCas9-dp300 fusion protein

   Depositor

   Stephan Beck

   Insert

   dp300
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   S1396R-S1397R

   Promoter

   CMV promoter

   Available Since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIC84

   Plasmid

   #63062

   Purpose

   Retroviral expression of EGFP-TEV-S-tag hCENP-H for protein localization and affinity purification (LAP)

   Depositor

   Iain Cheeseman

   Insert

   hCENP-H, IMAGE clone 3451767 (CENPH Human)
   

   Use

   Retroviral

   Tags

   EGFP and TEV-S

   Expression

   Mammalian

   Available Since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAM1518

   Plasmid

   #40238

   Depositor

   Susan Golden

   Article

   Andersson et al Methods Enzymol. 2000;305:527-42.

   Insert

   psbAI promoter, luxCDE
   

   Use

   Cyanobacteria cloning vector

   Promoter

   n/a

   Available Since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pBACgus4x/Parkin

   Plasmid

   #40385

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   Parkin (PRKN Human)
   

   Use

   Baculovirus

   Mutation

   WT

   Promoter

   polH

   Available Since

   Oct. 1, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pTF267 (pFA6a-TEV-6xGly-12xHis-KanMX)

   Plasmid

   #44093

   Depositor

   Tim Formosa

   Type

   Empty backbone

   Use

   Pcr-based yeast c-terminal tagging

   Tags

   12xHis tag, 6xGly linker, ADH1 transcription term…

   Available Since

   April 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKG402

    Plasmid

    #69760

    Purpose

    Retroviral expression of CENP-T aa 256-561

    Depositor

    Iain Cheeseman

    Insert

    CENP-T aa 256-561 (CENPT Human)
    

    Use

    Retroviral

    Tags

    mCherry-TEV-S

    Expression

    Mammalian

    Mutation

    e.coli optimized sequence

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pCVL TLR 3.1 (Sce target) Ef1a iRFP Puro

    Plasmid

    #50417

    Purpose

    Fluorescent reporter for DNA cleavage/repair in mammalian cells expressing iRFP with selectable puromycin marker.

    Depositor

    Andrew Scharenberg

    Inserts

    eGFP with I-Sce I TS
    +3 mCherry
    

    Use

    Lentiviral

    Tags

    T2A dislinker and iRFP

    Expression

    Mammalian

    Mutation

    embedded I-Sce I TS from 163-185

    Promoter

    SFFV

    Available Since

    Jan. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBluescript alpha-SSeCKS

    Plasmid

    #61295

    Purpose

    contains rat alpha SSeCKS cDNA

    Depositor

    Irwin Gelman

    Insert

    alpha SSeCKS (Akap12 Rat)
    

    Expression

    Bacterial

    Mutation

    cDNA with 1kb of 3' UTR and 150bp of 5'…

    Promoter

    T7

    Available Since

    Dec. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pPyCAG-Nanog(K140A)-IP

    Plasmid

    #62998

    Purpose

    Expresses Mouse Nanog K140A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

    Depositor

    Shinya Yamanaka

    Insert

    Mouse Nanog K140A Mutant (Nanog Mouse)
    

    Expression

    Mammalian

    Mutation

    Changed Lysine 140 (141 AA in Mouse Protein) to A…

    Available Since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV H2afy-V5

    Plasmid

    #175122

    Purpose

    Lentiviral expression of mouse Macroh2a1-V5

    Depositor

    Larry Gerace

    Insert

    Macroh2a1 (Macroh2a1 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. p2Bac.Flag.p34

    Plasmid

    #39221

    Depositor

    Aziz Sancar

    Insert

    p34 (GTF2H3 Human)
    

    Tags

    Flag

    Expression

    Insect

    Promoter

    Pph

    Available Since

    Aug. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

16. Hs_MSI2_RBD

    Plasmid

    #61113

    Purpose

    Express His tagged human MSI2 RNA binding domain

    Depositor

    Sean Ryder

    Insert

    MSI2 RNA binding domain (MSI2 Human)
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Available Since

    Jan. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. H2B-sfGFP-T2A-iCre

    Plasmid

    #178546

    Purpose

    pCMV-Histone H2B-sfGFP-T2A-iCre-bpA-FRT-Neo*-FRT

    Depositor

    Mario Capecchi

    Insert

    Histone H2B-sfGFP-T2A-iCre
    

    Use

    Cre/Lox and Mouse Targeting; Flp/frt

    Expression

    Mammalian

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. 13SA-csy4

    Plasmid

    #97037

    Purpose

    Spectinomycin resistant plasmid with a CDF origin that expresses Csy4 when IPTG induced, for coexpression with Series 371 vectors

    Depositor

    Chris Jeans

    Insert

    Cys4
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDON-AI-baIaa

    Plasmid

    #51474

    Purpose

    Express mouse TH2B and TH2A in mammalian cells

    Depositor

    Shunsuke Ishii

    Inserts

    histone cluster 1, H2aa (H2ac1 Mouse)
    histone cluster 1, H2ba (H2bc1 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTF280 (pFA6a-PSc-6xGly-2xStrep-HphMX)

    Plasmid

    #44076

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    2xStrep tag, 6xGly linker, ADH1 transcription ter…

    Available Since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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21. Lazarus Gata 3 flag

    Plasmid

    #80135

    Purpose

    retroviral expression of Gata3-Flag

    Depositor

    Ellen Rothenberg

    Insert

    GATA binding protein 3 (Gata3 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG and IRES-EGFP

    Expression

    Mammalian

    Available Since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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22. gamma1-EGFP

    Plasmid

    #119224

    Purpose

    GABAA receptor expression (gamma 1 subunit with EGFP tag at the C-terminus)

    Depositor

    Angel de Blas

    Insert

    GABRG1 (Gabrg1 Rat)
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. pUASp YFP Cdc42 CA

    Plasmid

    #52250

    Purpose

    Drosophila expression of YFP-Cdc42 constitutively active mutant

    Depositor

    Matthew Scott

    Insert

    Cdc42 (Cdc42 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    Q61L; CA mutation

    Promoter

    GAL4

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

24. PTPRO

    Plasmid

    #38969

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PTPRO (PTPRO Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

25. pPyCAG-Nanog(L122A)-IP

    Plasmid

    #62992

    Purpose

    Expresses Mouse Nanog L122A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

    Depositor

    Shinya Yamanaka

    Insert

    Mouse Nanog L122A Mutant (Nanog Mouse)
    

    Expression

    Mammalian

    Mutation

    Changed Leucine 122 (123 AA in Mouse Protein) to …

    Available Since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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26. mouse pGL3-ank3_1-promoter firefly luciferase

    Plasmid

    #86436

    Purpose

    Mouse pGL3-ank3_1-promoter firefly luciferase, ca 2000 base pairs around TSS exon 1e

    Depositor

    Hans-Georg Koenig, Jochen Prehn

    Insert

    ank3_1 promoter
    

    Tags

    n/a

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    ankyrin-3_1

    Available Since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. HaloTag-G protein gamma-10

    Plasmid

    #67041

    Purpose

    G protein gamma-10 tagged with HaloTag on amino terminus

    Depositor

    Catherine Berlot

    Insert

    HaloTag-G protein gamma-10 (GNG10 Human)
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pKG194

    Plasmid

    #69757

    Purpose

    Mammalian Expression of CENP-T aa 1-375

    Depositor

    Iain Cheeseman

    Insert

    CENP-T aa 1-375 (CENPT Human)
    

    Use

    Retroviral

    Tags

    LacI-NLS and eGFP

    Expression

    Mammalian

    Mutation

    e.coli optimized sequence

    Available Since

    Nov. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

29. GYG1

    Plasmid

    #39091

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    GYG1 (GYS1 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

30. COVID-SARS2 NSP10

    Plasmid

    #159616

    Purpose

    Bacterial expression plasmid for COVID-SARS2 NSP10 fused to GST

    Depositor

    Opher Gileadi

    Insert

    Covid-SARS2 Nsp10
    

    Tags

    GST-3C protease site

    Expression

    Bacterial

    Promoter

    T7 - LacO

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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