We narrowed to 8,688 results for: APP

1. pSB700 lenti gRNA Puro_TtoC and hairpin extension

   Plasmid

   #167927

   Purpose

   Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_Blasto

   Plasmid

   #167896

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. NAX_CAGGS_CAS9_m4_VPR_iRFP713

   Plasmid

   #167884

   Purpose

   PiggyBac compatible plasmid expressing dCas9-VPR

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-VPR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. NAX_CAGGS_CAS9_m4_KRAB_Puro

   Plasmid

   #167879

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-KRAB
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. NAX_CAGGS_CAS9_m4_KRAB_iRFP713

   Plasmid

   #167876

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-KRAB
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. NAX_CAGGS_CAS9_m4_KRAB_Hygro

   Plasmid

   #167875

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-KRAB
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. NAX_CAGGS_CAS9_m4_KRAB_EYFP

   Plasmid

   #167874

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-KRAB
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. NAX_CAGGS_CAS9_m4_KRAB_Blasto

   Plasmid

   #167872

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-KRAB
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMC3-TPK2

   Plasmid

   #176180

   Purpose

   yeast MoClo level-0 part plasmid type 3 containing TPK2 gene

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Insert

   TPK2 (TPK2 Budding Yeast)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMC6-spacer

    Plasmid

    #176178

    Purpose

    yeast MoClo level-0 part plasmid type 6 containing non-coding DNA spacer for construction of MoClo plasmids without yeast marker

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    41 bp non-coding DNA spacer from pYTK048
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET29b-AviTag-mEGFP-dspB(E184Q W330Y)-6xHis

    Plasmid

    #176575

    Purpose

    Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.

    Depositor

    Mark Nitz

    Article

    Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

    Insert

    Dispersin B
    

    Use

    Tags

    Avitag, Hexahistidine tag, and mEGFP

    Expression

    Bacterial

    Mutation

    E184Q W330Y

    Promoter

    T7

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. IBMc397

    Plasmid

    #161955

    Purpose

    Demonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase C-lobe fused to acVHH

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB3K3-TT-PBAD(SapI-)-B30-His-acVHH-15aa-T7RNAP(181+)-T
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a-anticalin (E60amb)-His

    Plasmid

    #168043

    Purpose

    Expresses anticalin targeting CTLA-4 with the codon encoding E60 changed to amber codon (TAG).

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Use

    Tags

    6x Histag

    Expression

    Bacterial

    Mutation

    Glu 60 was mutated to amber codon (TAG)

    Promoter

    T7 promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pACEBac1-Q22YU3-2xStrep

    Plasmid

    #170315

    Purpose

    Plasmid for insect cell expression of Tetrahymena thermophila Q22YU3 C-terminally tagged with 2x Strep-tag II

    Depositor

    Andrew Carter

    Article

    Mali et al Science. 2021 Feb 26;371(6532):910-916.

    Insert

    Q22YU3 (TTHERM_00122270 Tetrahymena thermophila)
    

    Use

    Tags

    Strep-Tag II

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a-anticalin (E143amb)-His

    Plasmid

    #168046

    Purpose

    Expresses anticalin targeting CTLA-4 with the codon encoding E143 changed to amber codon (TAG).

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Use

    Tags

    6x Histag

    Expression

    Bacterial

    Mutation

    Glu 143 was mutated to amber codon (TAG)

    Promoter

    T7 promoter

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a-anticalin (S87amb)-His

    Plasmid

    #168044

    Purpose

    Expresses anticalin targeting CTLA-4 with the codon encoding S87 changed to amber codon (TAG).

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Use

    Tags

    6x Histag

    Expression

    Bacterial

    Mutation

    Ser 87 was mutated to amber codon (TAG)

    Promoter

    T7 promoter

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-anticalin (N116amb)-His

    Plasmid

    #168045

    Purpose

    Expresses anticalin targeting CTLA-4 with the codon encoding N116 changed to amber codon (TAG).

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Use

    Tags

    6x Histag

    Expression

    Bacterial

    Mutation

    Asn 116 was mutated to amber codon (TAG)

    Promoter

    T7 promoter

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a-anticalin (N21amb)-His

    Plasmid

    #168041

    Purpose

    Expresses anticalin targeting CTLA-4 with the codon encoding N21 changed to amber codon (TAG).

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Use

    Tags

    6x Histag

    Expression

    Bacterial

    Mutation

    Asn 21 was mutated to amber codon (TAG)

    Promoter

    T7 promoter

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a-Fgβ-anticalin-His-ybbr

    Plasmid

    #168040

    Purpose

    Expresses anticalin targeting CTLA-4 with Fgβ peptide at the N terminus and 6x-His tag and ybbr tag at the C terminus

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Use

    Tags

    6x Histag, Fgβ peptide, and ybbr tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Ash1

    Plasmid

    #169711

    Purpose

    Bacterial Expression of yeast Ash1 truncation (encodes amino acids 420-500)

    Depositor

    Tanja Mittag

    Article

    Martin et al J Am Chem Soc. 2016 Nov 30;138(47):15323

    Insert

    Ash1 (ASH1 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Truncation including encoding amino acids 420-500

    Promoter

    T7

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge