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We narrowed to 6,447 results for: nls

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Showing: 4461 - 4480 of 6447 results

  6. PX380 Treponema denticola Cas9

    Plasmid
    #68317
    Purpose
    Treponema denticola Cas9
    Depositor
    Feng Zhang
    Insert
    TdCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 26, 2015
    Availability
    Academic Institutions and Nonprofits only
    68317-plasmid-map-sequence-id-119529

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  8. pYPQ230-RRV

    Plasmid
    #188543
    Purpose
    Gateway entry clone (attL1 & attR5) for LbCas12a-RRV mediated genome editing
    Depositor
    Yiping Qi
    Article
    Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:
    Insert
    LbCas12a-RRV
    Use
    CRISPR; Gateway compatible lbcas12a-rrv entry clo…
    Tags
    NLS
    Expression
    Plant
    Mutation
    G146R, D156R, R182V
    Available Since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    188543-plasmid-map-sequence-id-369473

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  10. pSpdCas9-huTET1CD-T2A-mCherry(PX458)

    Plasmid
    #129027
    Purpose
    targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-mCherry and cloning backbone for sgRNA
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-huTET1CD, SgRNA cloning site
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129027-plasmid-map-sequence-id-256129

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  11. 3xFLAG-dCas9/MSCV-EGFP

    Plasmid
    #82613
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al Genes Cells. 2018 Feb 26. doi: 10.1111/g
    Insert
    3xFLAG-dCas9
    Use
    Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available Since
    Sept. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    82613-plasmid-map-sequence-id-298740

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  13. pY130 (pcDNA3.1-U6-huBsCpf1)

    Plasmid
    #92306
    Purpose
    Expression of humanized BsCpf1 and crRNA
    Depositor
    Feng Zhang
    Insert
    huBsCpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available Since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92306-plasmid-map-sequence-id-181649

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  14. pY127 (pcDNA3.1-U6-huTsCpf1)

    Plasmid
    #92303
    Purpose
    Expression of humanized TsCpf1 and crRNA
    Depositor
    Feng Zhang
    Insert
    huTsCpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available Since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92303-plasmid-map-sequence-id-181636

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  15. pY111 (pcDNA3.1-huTsCpf1)

    Plasmid
    #92267
    Purpose
    Expression of humanized TsCpf1
    Depositor
    Feng Zhang
    Insert
    huTsCpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available Since
    July 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    92267-plasmid-map-sequence-id-183046

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  18. pY128 (pcDNA3.1-U6-huMb2Cpf1)

    Plasmid
    #92304
    Purpose
    Expression of humanized Mb2Cpf1 and crRNA
    Depositor
    Feng Zhang
    Insert
    huMb2Cpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available Since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92304-plasmid-map-sequence-id-181652

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  19. pEJS-HZ79 All-in-one AAV-U1a-NmeABE8e-2xBPSV40-U6-Rosa26_V2

    Plasmid
    #199261
    Purpose
    Optimized single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one U6 driven sgRNA targeting mouse Rosa26 gene. This optimized construct showed improved in vivo editing efficiency.
    Depositor
    Erik Sontheimer
    Article
    Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.
    Insert
    NmeABE8e
    Use
    AAV and CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Promoter
    U1a promoter
    Available Since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    199261-plasmid-map-sequence-id-389819

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Showing: 4461 - 4480 of 6447 results