We narrowed to 5,822 results for: pCas

1. XLone-Cas9-2A-GFP

   Plasmid

   #170159

   Purpose

   Express Cas9 and GFP upon doxycycline treatment

   Depositor

   Xiaojun Lian

   Article

   Ream et al Stem Cell Reports. 2024 Mar 5:S2213-6711

   Insert

   Cas9
   

   Use

   Synthetic Biology; Piggybac

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   TRE3G promoter

   Available Since

   July 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBluescriptSKII+ U6-sgRNA(F+E) ACTB

   Plasmid

   #74705

   Purpose

   Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of ACTB mRNA

   Depositor

   Eugene Yeo

   Article

   Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

   Insert

   U6 promoter driving sgRNA targeting the 3'UTR of ACTB mRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. HuEx-1xNLS-iNme2Cas9-npNLS_EGFP-T1

   Plasmid

   #222992

   Purpose

   Mammalian expression of iNme2Cas9 construct with EGFP-targeting sgRNA

   Depositor

   Jennifer Doudna

   Article

   Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

   Insert

   HuEx-1xNLS-iNme2Cas9-npNLS_EGFP-T1
   

   Use

   CRISPR

   Tags

   Puro

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTRANS_211

   Plasmid

   #91110

   Purpose

   Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: BeYDV

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   35S

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJB042 (FtsZ-mEos2)

   Plasmid

   #49764

   Purpose

   Inducible expression of FtsZ-mEos2 in bacteria

   Depositor

   Jie Xiao

   Article

   Buss et al Mol Microbiol. 2013 Sep;89(6):1099-120.

   Insert

   FtsZ-mEos2
   

   Tags

   mEos2

   Expression

   Bacterial

   Promoter

   T5-lac

   Available Since

   March 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. p2T-CMV-eA3Amax-BlastR

   Plasmid

   #152997

   Purpose

   C-to-T base editor

   Depositor

   David Liu

   Article

   Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

   Insert

   eA3A-nSpCas9-UGI-UGI
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. TagRFP-T-VAPB(1- 218)- eMagB

   Plasmid

   #162255

   Purpose

   Blue-light dependent heterodimerization system

   Depositor

   Pietro De Camilli

   Article

   Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

   Insert

   TagRFP-T-VAPB(1- 218)- eMagB
   

   Expression

   Mammalian

   Promoter

   beta-actin promoter

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. LMNA_mScarlet-I_Donor

   Plasmid

   #178092

   Purpose

   HDR donor plasmid to tag LMNA with mScarlet-I using eSpCas9(1.1)_No_FLAG_LMNA_G2.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   LMNA-mScarlet-I HDR donor
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. CP1028-CBEmax

   Plasmid

   #119802

   Purpose

   C-to-T base editor

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

   Insert

   rApobec1-nSpCas9 (CP1028) -UGI-UGI
   

   Expression

   Mammalian

   Available Since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pXEE401BG

    Plasmid

    #91717

    Purpose

    CRISPR/Cas9-mediated genome editing in Arabidopsis, Bar and glyphosate-resistance

    Depositor

    Qi-Jun Chen

    Article

    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTRANS_213

    Plasmid

    #91112

    Purpose

    Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: WDV

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    35S

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. SETMAR-Cas9

    Plasmid

    #186701

    Purpose

    Plasmid encoding SETMAR-Cas9 fusion under CMV promoter

    Depositor

    Jennifer Doudna

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

    Insert

    SETMAR-Cas9
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMZ-178

    Plasmid

    #195426

    Purpose

    Transient, constitutive expression of synZiFTR: ZF3-p65

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    pUb - 3XFLAG - NLS - ZF3-5 (ZF3) - p65 - RbGpA
    

    Expression

    Mammalian

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMZ-185

    Plasmid

    #195433

    Purpose

    Transient, constitutive expression of synZiFTR: ZF10-p65

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    pUb - 3XFLAG - NLS - ZF10-1 (ZF10) - p65 - RbGpA
    

    Expression

    Mammalian

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pL2-1 ConjEpi TpEF2nat TpEF2NoSQEVenusThrFLAG

    Plasmid

    #139961

    Purpose

    Over-expression of heterologus squalene epoxidase from Nannochloropsis oceanica

    Depositor

    Raffaela Abbriano

    Article

    Jaramillo-Madrid et al Pharmaceuticals (Basel). 2020 Dec 21;13(

    Insert

    ConjEpi TpEF2_nat_tTpEF2 TpEF2_NoSQEVenusThrFLAG_tTpEF2
    

    Use

    Synthetic Biology

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCB301TOR-CRISPR

    Plasmid

    #164797

    Purpose

    For gene editing in oomycetes

    Depositor

    Miaoying Tian

    Article

    Gumtow et al Mol Plant Microbe Interact. 2018 Mar;31(

    Inserts

    PsNLS-hSpCas9
    sgRNA expression cassette
    

    Use

    Oomycete expression

    Promoter

    HAM34 and RPL41

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. NAX_CAGGS_CAS9_Puro

    Plasmid

    #167863

    Purpose

    PiggyBac compatible plasmid expressing spCas9

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9
    

    Use

    CRISPR

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. p35S-A3A-PBE

    Plasmid

    #119773

    Purpose

    Conversion of C to T in potato

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

    Insert

    APOBEC3A-nCas9-NLS-UGI-NLS
    

    Tags

    NLS (flanking UGI)

    Expression

    Plant

    Mutation

    nCas9 has a mutation D10A in wild type SpCas9

    Promoter

    CaMv 2x35S

    Available Since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSCL.139

    Plasmid

    #184994

    Purpose

    Express Cas9-P2A-Eco1RT in human cells

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    SpCas9-P2A-Eco1RT
    

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    Human codon optimized RT

    Promoter

    TetOn-3g

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. ABE8r-R153del

    Plasmid

    #208296

    Purpose

    Expresses ABE8r in mammalian cells

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA8r-R153del-SpCas9 D10A
    

    Expression

    Mammalian

    Mutation

    W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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