We narrowed to 43,328 results for: cha;

1. pGPA1

   Plasmid

   #115476

   Purpose

   Generation of mariner-based transposon mutant libraries in Enterococcus faecium

   Depositor

   Willem van Schaik

   Article

   Zhang et al BMC Genomics. 2017 Nov 21;18(1):893. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Mfn1-Myc

   Plasmid

   #23212

   Depositor

   David Chan

   Article

   Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

   Insert

   Mfn1 (Mfn1 Mouse)
   

   Use

   Tags

   6xHis, 9xMyc, and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 28, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGL2-NOS2Promoter-Luciferase

   Plasmid

   #19296

   Depositor

   Charles Lowenstein

   Article

   Lowenstein et al Proc Natl Acad Sci U S A. 1993 Oct 15. 9

   Insert

   NOS2 Promoter (Nos2 Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMVTNT-T7-KPNA1

   Plasmid

   #26677

   Depositor

   Bryce Paschal

   Article

   Kelley et al BMC Cell Biol. 2010 . 11():63.

   Insert

   Karyopherin Alpha 1 (KPNA1 Human)
   

   Use

   Tags

   T7

   Expression

   Mammalian

   Mutation

   KPNA1 sequence contains a reported SNP at S73N

   Promoter

   Available since

   Nov. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET21-pelB-LaM-4

   Plasmid

   #172770

   Purpose

   Bacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaM-4
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV-mCherry-MHC-IIA

   Plasmid

   #35687

   Depositor

   Venkaiah Betapudi

   Article

   Dulyaninova et al Mol Biol Cell. 2007 Aug;18(8):3144-55. E

   Insert

   MHC IIA (MYH9 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET21-pelB-GST-4

   Plasmid

   #228986

   Purpose

   For bacterial expression of anti-GST nanobody GST-4, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   GST-4
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET21-pelB-GST-3

   Plasmid

   #228985

   Purpose

   For bacterial expression of anti-GST nanobody GST-3, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   GST-3
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET21-pelB-LaTdT-39

   Plasmid

   #228980

   Purpose

   For bacterial expression of anti-tdTomato nanobody LaTdT-39, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaTdT-39
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. UPB-stomach (UPB1)

    Plasmid

    #180784

    Purpose

    Unique Projection Barcode (UPB1)-Stomach for Projection-seq

    Depositor

    Rui Chang

    Article

    Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

    Insert

    humanized ChR2-H134R (partial)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. UPB-lung (UPB2)

    Plasmid

    #180788

    Purpose

    Unique Projection Barcode (UPB2)-Lung for Projection-seq

    Depositor

    Rui Chang

    Article

    Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

    Insert

    humanized ChR2-H134R (partial)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-hSyn1-mNeonGreen

    Plasmid

    #99135

    Purpose

    An AAV genome that ubiquitously expresses the fluorescent protein mNeonGreen from the hSyn1 promoter

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    mNeonGreen
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn1

    Available since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. UPB-colon (UPB4)

    Plasmid

    #180786

    Purpose

    Unique Projection Barcode (UPB4)-Colon for Projection-seq

    Depositor

    Rui Chang

    Article

    Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

    Insert

    hM3Dq ( partial )
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pREDTKI

    Plasmid

    #51628

    Purpose

    E coli Genetic Modification

    Depositor

    Sheng Yang

    Article

    Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

    Insert

    I-SceI (SCEI Budding Yeast)
    

    Use

    Red recombinase and i-scei endonuclease

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    May 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. NAX_CAGGS_CAS9_m4_KRAB_mcherry

    Plasmid

    #167878

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

16. pMC-6-BeYDV-Rep-RepA-9

    Plasmid

    #197715

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    BeYDV Rep/RepA proteins: native ORFs
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pETDuet1_His-TEV-EGFP

    Plasmid

    #223723

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    EGFP, not monomeric (has A207)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 3122 pSFFV-neo Bcl-XL MI-3

    Plasmid

    #8811

    Depositor

    Stanley Korsmeyer

    Article

    Chao et al J Exp Med 1995 Sep 1;182(3):821-8.

    Insert

    Bcl-XL MI-3 (BCL2L1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    BH1 WGR-->WAR

    Promoter

    Available since

    July 6, 2005

    Availability

    Academic Institutions and Nonprofits only

19. pJL1-sfGFP-DQNAT

    Plasmid

    #198210

    Purpose

    pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 30 amino acids containing an optimal DQNAT sequon followed by a 6x-His tag

    Depositor

    Michael Jewett

    Article

    Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

    Insert

    sfGFP_DQNAT
    

    Use

    Tags

    6x His Tag and DQNAT glycosylation tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET14b – E. coli Exonuclease I

    Plasmid

    #104552

    Purpose

    bacterial expression of Exonuclease I

    Depositor

    Charles Bell

    Article

    Korada et al Nucleic Acids Res. 2013 Jun;41(11):5887-

    Insert

    Exonuclease I (sbcB E.coli)
    

    Use

    Tags

    6X-His tag followed by thrombin site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCMV-calpainsensor

    Plasmid

    #36182

    Purpose

    detection of activity of ubiquitous calpains

    Depositor

    Isabelle Richard

    Article

    Stockholm et al J Mol Biol. 2005 Feb 11;346(1):215-22. E

    Insert

    alpha-Fodrin cleavage site (Sptan1 Mouse)
    

    Use

    Tags

    eCFP and eYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pλPR cI-Elysis

    Plasmid

    #176891

    Purpose

    Bacterial expression plasmids for production of E. coli bacterial ghost (BG) capsules

    Depositor

    James Inglese

    Article

    Dranchak et al Dis Model Mech. 2023 Mar 1;16(3):dmm0498

    Insert

    Lysis E gene from PhiX174 and lambda PR-cI857 regulatory system
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-hSyn1-mTurquoise2

    Plasmid

    #99125

    Purpose

    An AAV genome that expresses the fluorescent protein mTurquoise2 from the hSyn1 promoter

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    mTurquoise2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn1

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-hSyn1-tTA

    Plasmid

    #99119

    Purpose

    An AAV genome that expresses tTA from the hSyn1 promoter

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    tTA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    *(see below)

    Promoter

    hSyn1

    Available since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJL1-sfGFP-AQNAT

    Plasmid

    #198211

    Purpose

    pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 30 amino acids containing a non-permissible AQNAT sequon followed by a 6x-His tag

    Depositor

    Michael Jewett

    Article

    Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

    Insert

    sfGFP_AQNAT
    

    Use

    Tags

    6x His Tag and AQNAT mutated (non-permissible) gl…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJL1-sfGFP-MOOR

    Plasmid

    #198212

    Purpose

    pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 32 amino acids containing the minimum optimal O-linked recognition site (MOOR) followed by a 6x-His tag

    Depositor

    Michael Jewett

    Article

    Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

    Insert

    sfGFP_MOOR
    

    Use

    Tags

    6x His Tag and MOOR glycosylation tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pmGFP-Sec16S

    Plasmid

    #15775

    Depositor

    Benjamin Glick

    Article

    Bhattacharyya et al Mol Biol Cell. 2007 Mar;18(3):839-49. do

    Insert

    Sec16S (SEC16B Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    Nov. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-EF1α-FRT-FLEX-GtACR1-EYFP

    Plasmid

    #114370

    Purpose

    expresses Flpo recombinase-dependent GtACR1

    Depositor

    Mingshan Xue

    Article

    Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

    Insert

    GtACR1-EYFP (NEWENTRY )
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. EKARdual

    Plasmid

    #99870

    Purpose

    Measure ERK activity by conformational change after ERK substrate phosphorylation and recognition of this phosphorylated substrate

    Depositor

    Marc Tramier

    Article

    Demeautis et al Sci Rep. 2017 Jan 20;7:41026. doi: 10.10

    Insert

    EKAR
    

    Use

    Tags

    ShadowG and mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pIS1-Eef25UTR-TOPmut-renilla

    Plasmid

    #38236

    Depositor

    David Sabatini

    Article

    Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

    Insert

    Eef2_TOPm_5UTR/Renilla (Eef2 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    The first nucleotides of the 5' UTR were cha…

    Promoter

    Endogenous Eef2

    Available since

    Nov. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

31. phage ubc nls ha pcp gfp

    Plasmid

    #64539

    Purpose

    Used to label reporter mRNAs. Lentiviral expression of the PP7 coat protein fused to EGFP.

    Depositor

    Jeffrey Chao

    Article

    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

    Insert

    PCP
    

    Use

    Lentiviral

    Tags

    EGFP, FactorXa site, HA, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    human ubiquitin C promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Lenti-mApple-6xHp

    Plasmid

    #217376

    Purpose

    Lipid droplet localization of 6xHp of Spastin fused to mApple

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    mApple-6x Hp NC
    

    Use

    Lentiviral

    Tags

    mApple

    Expression

    Mutation

    Promoter

    CMV

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. phage ubc nls ha 2xmcp HALO

    Plasmid

    #64540

    Purpose

    Used to label reporter mRNAs. Lentiviral expression of the MCP protein fused to HALO.

    Depositor

    Jeffrey Chao

    Article

    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

    Insert

    MCP
    

    Use

    Lentiviral

    Tags

    HA, HALO, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    human ubiquitin C promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMax_mPlum

    Plasmid

    #177830

    Purpose

    Host Cell Reactivation (HCR) control mPlum plasmid (pMax backbone)

    Depositor

    Zachary Nagel, Leona Samson

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    mPlum
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. FAB(ER-mito)

    Plasmid

    #214427

    Purpose

    Detection of ER-mito contact site

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    FRB-V5
    

    Use

    Tags

    CFAST10-Halo and RspAN-FRB-V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. iSpyMac

    Plasmid

    #155014

    Purpose

    Expresses the codon-optimized, engineered iSpyMac Cas9 nuclease in mammalian cells

    Depositor

    Joseph Jacobson

    Article

    Chatterjee et al Nat Commun. 2020 May 18;11(1):2474. doi:

    Insert

    iSpyMac
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    PID grafted onto SpyCas9 N-terminal domain, R221K…

    Promoter

    EF1ɑ

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. TRE-UBC-H2-Kb SCT-T2A-GFP

    Plasmid

    #205454

    Purpose

    lentiviral plasmid for expression of mouse H2-Kb SCT

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    H2-Kb SCT (H2-K1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. piggyFlex

    Plasmid

    #218234

    Purpose

    A piggyBac transposon-based gRNA expression vector, to allow for genomic integration and stable expression of gRNAs. Contains both antibiotic (puromycin) and fluorophore (GFP) markers.

    Depositor

    Jay Shendure

    Article

    Chardon et al bioRxiv [Preprint]. 2023 Mar 28:2023.03.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. mCer-TG2-YFP

    Plasmid

    #85471

    Purpose

    TG2 biosensor that allows for quantitative assessment of TG2 conformational changes in live cells using FRET

    Depositor

    Ray Truant, Jeffrey Keillor

    Article

    Caron et al PLoS One. 2012;7(8):e44159. doi: 10.1371

    Insert

    hTG2 (TGM2 Human)
    

    Use

    Tags

    EYFP and mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMpGE_En03

    Plasmid

    #71535

    Purpose

    Used to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorpha

    Depositor

    Sumihare Noji, Takayuki Kohchi

    Article

    Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge